FastQCFastQC Report
Mon 15 Aug 2016
SRR2921090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000800
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98710.16449473403546194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.13471537128382882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88600.051.715231
GTATCAA147100.047.7369581
TCAACGC179700.038.2432634
CAACGCA182500.037.7218365
ATCAACG186300.036.9524153
TATCAAC188250.036.8549082
AACGCAG191550.035.9236456
GTACATG195700.032.315141
ACGCAGA219400.031.2076477
TACATGG204400.029.8312952
CGCAGAG233850.029.076258
ACATGGG204800.028.6086463
CATGGGG155100.028.3992164
GCAGAGT258350.025.8125179
GAGTACT146550.023.30095712-13
AGAGTAC228250.023.27366610-11
CAGAGTA241600.022.52933310-11
AGTACTT160800.020.99556212-13
GTACTTT164900.020.76266914-15
ATGGGGG83850.019.4058785
Produced by FastQC (version 0.11.5)