FastQCFastQC Report
Mon 15 Aug 2016
SRR2921089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647607
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40650.1535348713007633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83550.028.4130251
TACATGG120500.028.103512
GTACATG123250.027.9718861
CATGGGG79150.026.6197574
AACGCAG91450.025.4445936
ACATGGG132750.025.2869423
GGTATCA64150.024.7323721
TCAACGC93900.024.593364
CAACGCA95800.024.1051455
TATCAAC98950.023.5177762
ATCAACG99300.023.2559573
ACGCAGA100550.022.843717
CGCAGAG104250.021.7471398
GTATAGA7800.021.4113621
TACAGCG2659.22264E-720.2134365
CCTACGA8200.019.5968022
GCAGAGT108600.019.5610249
ACCGTCG3152.3110442E-718.8904678
ATACCGT2851.853623E-618.7931736
ATGGGGA36850.018.412425
Produced by FastQC (version 0.11.5)