##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921089_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2647607
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.17955497171597	31.0	30.0	34.0	22.0	34.0
2	29.279115442737535	31.0	30.0	34.0	22.0	34.0
3	29.37811654071016	31.0	30.0	34.0	22.0	34.0
4	32.68296012210271	37.0	35.0	37.0	23.0	37.0
5	32.781206198654104	37.0	35.0	37.0	25.0	37.0
6	32.75860541235916	37.0	35.0	37.0	25.0	37.0
7	32.630663085571236	37.0	33.0	37.0	23.0	37.0
8	32.605078095049606	37.0	35.0	37.0	22.0	37.0
9	33.96597644590001	39.0	35.0	39.0	21.0	39.0
10-11	33.926273990059705	39.0	35.0	39.0	21.5	39.0
12-13	33.85887123730977	39.0	35.0	39.0	20.0	39.0
14-15	35.018078400608545	40.0	35.0	41.0	20.0	41.0
16-17	35.00643335661222	40.0	35.0	41.0	21.5	41.0
18-19	35.02483450149512	40.0	35.0	41.0	21.0	41.0
20-21	35.03963220372208	40.0	35.0	41.0	22.0	41.0
22-23	34.97236221236762	40.0	35.0	41.0	21.5	41.0
24-25	34.953446829533235	40.0	35.0	41.0	22.0	41.0
26-27	34.858853107730866	40.0	34.5	41.0	22.0	41.0
28-29	34.70427106439891	40.0	34.0	41.0	20.0	41.0
30-31	34.59080841680809	40.0	34.0	41.0	18.5	41.0
32-33	34.44471384914755	39.0	34.0	41.0	17.5	41.0
34-35	34.31200476505765	39.0	34.0	41.0	16.0	41.0
36-37	34.186647036361514	39.0	34.0	41.0	15.5	41.0
38-39	34.03791064912579	39.0	33.5	41.0	14.0	41.0
40-41	33.888611489545085	39.0	33.0	41.0	9.0	41.0
42-43	33.70368393043228	39.0	33.0	41.0	8.5	41.0
44-45	33.521685431410326	39.0	33.0	41.0	7.5	41.0
46-47	33.32925052698531	38.5	33.0	41.0	4.0	41.0
48-49	33.08103053058856	38.0	32.5	40.5	2.0	41.0
50-51	32.9101845553362	38.0	32.0	40.0	2.0	41.0
52-53	32.72102581689805	38.0	32.0	40.0	2.0	41.0
54-55	32.47202303816239	38.0	32.0	40.0	2.0	41.0
56-57	32.226296425413594	37.0	31.0	40.0	2.0	41.0
58-59	31.92886104319863	37.0	31.0	40.0	2.0	41.0
60-61	31.62414512425749	36.5	31.0	40.0	2.0	41.0
62-63	31.329950781970282	36.0	30.5	39.5	2.0	41.0
64-65	31.003779450651095	35.5	30.0	39.0	2.0	41.0
66-67	30.652020484913358	35.0	30.0	39.0	2.0	40.5
68-69	30.27294156572331	35.0	29.0	38.0	2.0	40.0
70-71	29.912588424188336	35.0	29.0	37.0	2.0	40.0
72-73	29.55223943734852	35.0	29.0	37.0	2.0	39.0
74-75	29.4961976985255	35.0	29.0	36.5	2.0	39.0
76-77	29.540265039335523	35.0	30.0	36.0	2.0	39.0
78-79	29.366280758435824	35.0	30.0	36.0	2.0	37.5
80-81	29.15474672034029	35.0	30.0	35.0	2.0	37.0
82-83	28.91462989031227	35.0	29.0	35.0	2.0	37.0
84-85	28.666091870885673	35.0	29.0	35.0	2.0	36.0
86-87	28.520152915444022	34.0	29.0	35.0	2.0	36.0
88-89	28.343942851034917	34.0	29.0	35.0	2.0	36.0
90-91	28.19674898880385	34.0	29.0	35.0	2.0	35.0
92-93	28.080580123862795	34.0	29.0	35.0	2.0	35.0
94-95	27.963284014583735	34.0	29.0	35.0	2.0	35.0
96-97	27.858974726989317	34.0	29.0	35.0	2.0	35.0
98-99	27.749976677052146	34.0	28.0	35.0	2.0	35.0
100-101	27.617400920907066	34.0	27.5	35.0	2.0	35.0
102-103	26.363906161299617	32.5	24.5	34.5	2.0	35.0
104-105	27.279619860500446	34.0	27.0	35.0	2.0	35.0
106-107	27.415456108100635	34.0	27.0	35.0	2.0	35.0
108-109	27.34801407459642	34.0	27.0	35.0	2.0	35.0
110-111	27.243747467052323	34.0	27.0	35.0	2.0	35.0
112-113	27.120420628892433	34.0	26.5	35.0	2.0	35.0
114-115	26.969760806645397	34.0	25.0	35.0	2.0	35.0
116-117	26.814154819805204	34.0	25.0	35.0	2.0	35.0
118-119	26.591475056532182	34.0	25.0	35.0	2.0	35.0
120-121	26.363074089168066	34.0	24.5	35.0	2.0	35.0
122-123	26.03836502169695	34.0	23.5	35.0	2.0	35.0
124-125	24.754191237596817	32.5	10.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133730.0
3	18489.0
4	12490.0
5	13803.0
6	19839.0
7	18895.0
8	15913.0
9	16564.0
10	17219.0
11	17447.0
12	17722.0
13	17421.0
14	17240.0
15	15314.0
16	12671.0
17	11241.0
18	11019.0
19	10994.0
20	11221.0
21	12146.0
22	14496.0
23	18446.0
24	24426.0
25	30066.0
26	33292.0
27	37033.0
28	43080.0
29	50100.0
30	59237.0
31	71347.0
32	88030.0
33	113553.0
34	154445.0
35	231864.0
36	426533.0
37	642335.0
38	187748.0
39	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.075994588264166	18.047616306111536	11.773101006443481	25.103288099180816
2	16.654364228516183	21.030932978357914	37.197281595817095	25.117421197308808
3	19.41840344056238	24.818170753556643	28.51084852136404	27.25257728451694
4	13.421531015290194	16.860340943842207	34.782705663862195	34.935422377005395
5	14.142326384583514	38.39813070262718	33.0002736958619	14.459269216927408
6	33.17792882907345	36.0227842567472	16.463750840492807	14.335536073686544
7	28.4726121696885	33.19830279737441	20.145867766876265	18.183217266060826
8	26.411340866938037	35.29205795216281	19.39128675657331	18.90531442432584
9	26.568527461922464	16.437711744069652	18.510123441663858	38.483637352344026
10-11	25.19629623188362	26.69383005252966	26.782356174824628	21.32751754076209
12-13	25.50997108777702	24.534013445998255	26.63211540090003	23.323900065324686
14-15	23.360887770975232	25.83405749664509	23.912134053356766	26.892920679022907
16-17	23.417163493766047	28.279125019707358	24.823749097809372	23.479962388717222
18-19	23.492973156072527	27.35870134760251	26.299835698801804	22.848489797523158
20-21	24.625240911156425	26.561867950820478	25.710257881735966	23.102633256287127
22-23	24.19050138907263	26.39464781811653	25.515657777861772	23.899193014949066
24-25	23.78654971036569	26.538921494708482	25.801392157503905	23.873136637421922
26-27	23.642706590674948	26.607347756637463	25.80790351697999	23.942042135707602
28-29	23.71965173748487	26.74660047856703	25.918908992686156	23.614838791261946
30-31	23.929947373206055	26.779218663260913	25.6106857171263	23.680148246406727
32-33	23.55973110822021	26.904621752318175	25.597288398569567	23.93835874089205
34-35	23.78132663549865	26.860242557082874	25.65930865069353	23.699122156724947
36-37	23.82548692752949	26.706838278421063	25.62699072615301	23.840684067896436
38-39	23.680633465280067	26.753668099029603	25.736983994024598	23.828714441665735
40-41	23.762648167214863	26.742730357737265	25.824064799747315	23.67055667530056
42-43	23.66961549554576	26.650329224780446	25.762656508875402	23.917398770798393
44-45	23.559154211658008	26.78329749649932	25.701464210446538	23.95608408139614
46-47	23.501309888691114	26.77071193579495	25.700104938496743	24.027873237017193
48-49	23.404953742330942	26.685199341214442	25.780058980772193	24.129787935682423
50-51	23.218335759603296	26.845210905949614	25.673646626111452	24.262806708335642
52-53	23.47728772336292	26.638924028681632	25.455070553962532	24.42871769399292
54-55	23.36214216101743	26.569679131002168	25.63241563948112	24.435763068499284
56-57	23.43383067517042	26.509600936247	25.616132302544187	24.440436086038392
58-59	23.32609109348121	26.23758867995769	25.72190743854317	24.714412788017935
60-61	23.434566539911735	26.091373551798625	25.72876209153761	24.745297816752025
62-63	23.242904848502377	26.086409189035624	25.81955244506216	24.85113351739984
64-65	23.08644878006739	26.065442336609625	25.826827252885494	25.021281630437493
66-67	23.18700288361991	26.113769370128054	25.794507401442303	24.904720344809732
68-69	23.397409303411262	25.824313839634616	25.85772998882033	24.92054686813379
70-71	23.114905455861603	25.59098001007387	26.099549888818853	25.19456464524567
72-73	23.07930117788096	25.601053388250218	26.263824020063964	25.055821413804857
74-75	22.95839604928713	25.807469283438056	26.254185185318203	24.979949481956613
76-77	22.860905630460717	25.43112500522195	26.578592639065924	25.12937672525141
78-79	22.967201753412812	25.51412571177587	26.53312586807409	24.985546666737225
80-81	23.02787306878588	25.478294284549396	26.470900113958574	25.02293253270615
82-83	22.87425944289464	25.324094744431008	26.646181275298787	25.155464537375572
84-85	22.999071845930207	25.275166491180645	26.52728602835123	25.198475634537914
86-87	22.896624721798258	25.058106952326497	26.669734958204767	25.375533367670478
88-89	22.98637819059195	25.051249024907936	26.61211442289667	25.350258361603444
90-91	22.975926007484006	25.026212172390373	26.635857873792574	25.362003946333044
92-93	22.918993405196247	24.944715747668724	26.731594246280338	25.40469660085469
94-95	22.632183727711833	24.927721585911744	26.831769876492988	25.60832480988343
96-97	22.657790078931423	24.947050816473954	26.761290376233106	25.63386872836152
98-99	22.503646129716227	25.088509716177366	26.722119463028143	25.68572469107827
100-101	22.471154017002316	25.250295916820438	26.734473298303435	25.544076767873815
102-103	22.184007658702456	25.425558009266485	26.764130181312147	25.62630415071891
104-105	21.980196560308002	25.527175774142364	26.672958632529287	25.819669033020347
106-107	21.875465463989627	25.706522355502965	26.76689185357684	25.651120326930567
108-109	21.82487286183598	25.75622704325108	26.773313452213003	25.645586642699936
110-111	21.909633434699177	25.963063718337036	26.67567416350497	25.451628683458814
112-113	21.903721872763914	26.12073302708009	26.594004285731028	25.381540814424973
114-115	21.739464926880398	26.29804897827252	26.542628449588445	25.419857645258638
116-117	21.637935466506182	26.176396539705532	26.629101009933	25.556566983855284
118-119	21.73694053299087	26.10082809102481	26.61907718360223	25.54315419238209
120-121	21.757611059467344	26.1840712468482	26.567684562508592	25.490633131175866
122-123	21.904466677125644	26.300545694082423	26.474309660127282	25.320677968664647
124-125	21.829829349148447	26.394193730734074	26.295090893107137	25.480886027010346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3144.0
1	2216.5
2	1115.5
3	859.5
4	704.0
5	560.0
6	472.5
7	500.5
8	612.0
9	637.5
10	572.0
11	569.5
12	614.0
13	662.5
14	771.5
15	915.5
16	1108.5
17	1295.0
18	1533.0
19	1812.5
20	2171.5
21	2692.0
22	3329.0
23	4151.0
24	5081.5
25	6289.0
26	8159.5
27	10479.5
28	12397.0
29	14947.5
30	17841.0
31	20313.0
32	24016.5
33	28354.0
34	32465.5
35	36829.5
36	42250.0
37	47038.0
38	50989.0
39	55194.0
40	60078.0
41	65540.5
42	71184.0
43	77939.0
44	84895.5
45	92247.5
46	99089.0
47	105703.5
48	112089.5
49	116747.5
50	121520.0
51	123532.0
52	120601.0
53	117472.0
54	114241.5
55	108548.0
56	100442.0
57	90117.5
58	81268.5
59	71854.0
60	60168.0
61	49658.5
62	39997.0
63	30377.5
64	22014.0
65	16228.5
66	11984.5
67	8857.0
68	6660.5
69	5030.5
70	3869.5
71	2982.5
72	2323.0
73	1957.5
74	1583.5
75	1202.0
76	881.0
77	584.0
78	419.0
79	312.5
80	245.5
81	199.0
82	137.0
83	105.0
84	84.0
85	65.5
86	53.0
87	48.0
88	32.5
89	20.0
90	21.0
91	24.5
92	26.5
93	24.0
94	15.0
95	16.0
96	24.5
97	21.5
98	23.5
99	34.5
100	56.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28021530385740784
2	0.06760822131079121
3	0.07527552238681949
4	0.08256512390245228
5	0.08819284735234496
6	0.12558510383149765
7	0.11406526723943547
8	0.11376310759111909
9	0.12086385932655412
10-11	0.09914613460381394
12-13	0.08909932629729413
14-15	0.098220770680845
16-17	0.10031700324103993
18-19	0.09310294163748623
20-21	0.09219646269253708
22-23	0.07391580396939576
24-25	0.08643654439650597
26-27	0.09752202649411336
28-29	0.09134663868164723
30-31	0.08617215470422915
32-33	0.09548244886797776
34-35	0.08966587563788735
36-37	0.08960922070382803
38-39	0.0791280579028534
40-41	0.0910822489893704
42-43	0.08120540548502855
44-45	0.07748506481513305
46-47	0.06690947712405958
48-49	0.06910013457435336
50-51	0.07227281088167542
52-53	0.0838681873858167
54-55	0.08887270656105684
56-57	0.09738983164797495
58-59	0.0674760264646528
60-61	0.08360379769353987
62-63	0.08942037092363028
64-65	0.08056331623235624
66-67	0.07455789322206807
68-69	0.08231961918819523
70-71	0.08118652050700878
72-73	0.06303805663000589
74-75	0.09115778890144949
76-77	0.09523694415372069
78-79	0.07642750604602572
80-81	0.08866497180283932
82-83	0.09329179141768397
84-85	0.09714432693371788
86-87	0.08571891523175455
88-89	0.06432223513535053
90-91	0.08938260096759074
92-93	0.06556864368465562
94-95	0.09266858714303142
96-97	0.08105432566087037
98-99	0.07529440736483926
100-101	0.09208315282441842
102-103	0.06526648403633924
104-105	0.07195176625533926
106-107	0.09053458462679695
108-109	0.09637004283490715
110-111	0.09076120436303424
112-113	0.07871258838641837
114-115	0.09270635709907096
116-117	0.08622880963828847
118-119	0.07797607424364719
120-121	0.10902297810815578
122-123	0.08883493660501729
124-125	0.10875858841587895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2647607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.664394870453954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12768868544134	31.566740726138054
2	13.480655124817527	12.311719174641528
3	6.090442430394505	8.343491042319059
4	3.3316292468395208	6.08547333958532
5	2.027118028614866	4.6283559053842716
6	1.3360562034955261	3.6606118827323573
7	0.9131188022468878	2.918791228460649
8	0.6846586589276623	2.501161868219858
9	0.4875425001520737	2.003699991876539
>10	2.3658194022904473	18.681241927364248
>50	0.11301700178001034	3.513853154590569
>100	0.04058865148058713	2.959790486418785
>500	0.001165684463387809	0.3525365111752
>1k	4.995790557341383E-4	0.47253276109368164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4065	0.1535348713007633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.7769956039548164E-5	0.0	0.0	0.0	0.0
7	7.553991207909633E-5	0.0	0.0	0.0	0.0
8	7.553991207909633E-5	0.0	0.0	0.0	0.0
9	7.553991207909633E-5	0.0	0.0	3.7769956039548164E-5	0.0
10-11	7.553991207909633E-5	0.0	0.0	7.553991207909633E-5	0.0
12-13	1.133098681186445E-4	0.0	0.0	7.553991207909633E-5	0.0
14-15	1.5107982415819265E-4	0.0	0.0	1.133098681186445E-4	0.0
16-17	1.5107982415819265E-4	0.0	0.0	1.3219484613841858E-4	0.0
18-19	2.0773475821751492E-4	0.0	0.0	1.6996480217796673E-4	0.0
20-21	2.6438969227683717E-4	0.0	0.0	2.6438969227683717E-4	0.0
22-23	2.6438969227683717E-4	0.0	0.0	5.287793845536743E-4	0.0
24-25	4.1546951643502985E-4	0.0	0.0	0.0011708686372259931	0.0
26-27	4.1546951643502985E-4	0.0	0.0	0.002511702076629953	0.0
28-29	5.476643625734484E-4	0.0	0.0	0.008705974867115853	0.0
30-31	6.420892526723188E-4	0.0	0.0	0.026929978656197843	0.0
32-33	6.420892526723188E-4	0.0	0.0	0.07306597995850593	0.0
34-35	7.176291647514152E-4	0.0	0.0	0.15232623270749773	0.0
36-37	8.687089889096078E-4	0.0	0.0	0.29245276961422145	0.0
38-39	0.0010197888130678006	0.0	0.0	0.5120661790061742	0.0
40-41	0.0010197888130678006	0.0	0.0	0.7564566795600707	0.0
42-43	0.0010764437471271228	0.0	0.0	0.9835296552698343	0.0
44-45	0.001133098681186445	0.0	0.0	1.260307893127643	0.0
46-47	0.0012652935273248635	0.0	0.0	1.5896241398364634	0.0
48-49	0.0014352583295028302	0.0	0.0	1.9324431458294224	0.0
50-51	0.0014352583295028302	0.0	0.0	2.293335075787305	0.0
52-53	0.0014352583295028302	0.0	0.0	2.648127157844801	0.0
54-55	0.0015107982415819266	0.0	0.0	3.038385228623432	0.0
56-57	0.0015674531756412488	0.0	0.0	3.499103152393841	0.0
58-59	0.001586338153661023	0.0	0.0	3.9987807858190436	0.0
60-61	0.001586338153661023	0.0	0.0	4.526238221911333	0.0
62-63	0.001586338153661023	0.0	0.0	5.041325997400671	0.0
64-65	0.001586338153661023	0.0	0.0	5.59153983200679	0.0
66-67	0.0016618780657401194	0.0	0.0	6.229984283921292	0.0
68-69	0.0016618780657401194	0.0	0.0	6.940191652310936	0.0
70-71	0.0017185329997994416	0.0	0.0	7.721633157791167	0.0
72-73	0.0017563029558389897	0.0	0.0	8.485096164196575	0.0
74-75	0.0017940729118785378	0.0	0.0	9.235717385548536	0.0
76-77	0.001812957889898312	0.0	0.0	10.076646571791056	0.0
78-79	0.001812957889898312	0.0	0.0	11.015513254044123	0.0
80-81	0.002058462604155375	0.0	0.0	11.995228143753963	0.0
82-83	0.0021528874942542456	0.0	0.0	13.015621276118395	0.0
84-85	0.002190657450293794	0.0	0.0	14.015354242529197	0.0
86-87	0.002228427406333342	0.0	0.0	15.056483080759342	0.0
88-89	0.002228427406333342	0.0	0.0	16.173888345211353	0.0
90-91	0.002228427406333342	0.0	0.0	17.35453940105159	0.0
92-93	0.002228427406333342	0.0	0.0	18.55005293459339	0.0
94-95	0.002228427406333342	0.0	0.0	19.73625617397144	0.0
96-97	0.002228427406333342	0.0	0.0	20.9197966314487	0.0
98-99	0.0023606222524717602	0.0	0.0	22.169868866489626	0.0
100-101	0.002436162164550857	0.0	0.0	23.494876694313014	0.0
102-103	0.002511702076629953	3.7769956039548164E-5	0.0	24.831555438552623	0.0
104-105	0.0026061269667288236	3.7769956039548164E-5	0.0	26.124590998588538	0.0
106-107	0.0026438969227683714	3.7769956039548164E-5	0.0	27.392811697506467	0.0
108-109	0.0026438969227683714	3.7769956039548164E-5	0.0	28.705166590056606	0.0
110-111	0.0026438969227683714	5.665493405932224E-5	0.0	30.058464114953615	0.0
112-113	0.0026438969227683714	7.553991207909633E-5	0.0	31.418824621630023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8355	0.0	28.413025	1
TACATGG	12050	0.0	28.10351	2
GTACATG	12325	0.0	27.971886	1
CATGGGG	7915	0.0	26.619757	4
AACGCAG	9145	0.0	25.444593	6
ACATGGG	13275	0.0	25.286942	3
GGTATCA	6415	0.0	24.732372	1
TCAACGC	9390	0.0	24.59336	4
CAACGCA	9580	0.0	24.105145	5
TATCAAC	9895	0.0	23.517776	2
ATCAACG	9930	0.0	23.255957	3
ACGCAGA	10055	0.0	22.84371	7
CGCAGAG	10425	0.0	21.747139	8
GTATAGA	780	0.0	21.411362	1
TACAGCG	265	9.22264E-7	20.213436	5
CCTACGA	820	0.0	19.596802	2
GCAGAGT	10860	0.0	19.561024	9
ACCGTCG	315	2.3110442E-7	18.890467	8
ATACCGT	285	1.853623E-6	18.793173	6
ATGGGGA	3685	0.0	18.41242	5
>>END_MODULE