FastQCFastQC Report
Mon 15 Aug 2016
SRR2921089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647607
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.29891143209698423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65680.24807307126775233No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT65240.2464111932020122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.18110693920963344No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT41540.15689639738828307RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT28500.10764437471271228RNA PCR Primer, Index 39 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76800.042.72371
GTATCAA112150.038.3001631
TCAACGC140950.030.3592454
ATCAACG142550.030.1857223
CAACGCA142200.030.0923755
TATCAAC144400.029.757722
AACGCAG146000.029.4351086
ACGCAGA165100.025.7628617
TACATGG121500.025.3598022
GTACATG124400.025.1771371
CATGGGG75950.024.953474
CGCAGAG170950.024.7429818
GAGTACT97600.024.29014212-13
AGTACTT100350.023.5948512-13
ACATGGG131500.023.114053
GCAGAGT180650.022.7219859
GTACTTT106200.022.23995814-15
GTACCGT5450.021.8431266
AGAGTAC163950.021.15418810-11
TACCGTA4251.8189894E-1220.9960447
Produced by FastQC (version 0.11.5)