##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2647607
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331795466623255	33.0	31.0	34.0	28.0	34.0
2	31.370590876969278	33.0	31.0	34.0	28.0	34.0
3	31.468328192212816	33.0	31.0	34.0	28.0	34.0
4	34.97581363095051	37.0	35.0	37.0	32.0	37.0
5	34.74117419994735	37.0	35.0	37.0	32.0	37.0
6	34.49352113059076	37.0	35.0	37.0	31.0	37.0
7	34.430909496764436	37.0	35.0	37.0	30.0	37.0
8	34.41937190829304	37.0	35.0	37.0	30.0	37.0
9	35.977185435753874	39.0	37.0	39.0	30.0	39.0
10-11	35.95127165776492	39.0	37.0	39.0	30.0	39.0
12-13	35.90535000096313	39.0	37.0	39.0	30.0	39.0
14-15	37.14674383320485	40.0	37.0	41.0	31.0	41.0
16-17	37.141015830521674	40.0	37.0	41.0	31.0	41.0
18-19	37.16865040770779	40.0	37.0	41.0	31.0	41.0
20-21	37.172950139503335	40.0	37.0	41.0	31.0	41.0
22-23	37.13303220606382	40.0	37.0	41.0	30.5	41.0
24-25	37.08972120862349	40.0	37.0	41.0	30.0	41.0
26-27	36.964139126388474	40.0	37.0	41.0	30.0	41.0
28-29	36.82864904043538	40.0	37.0	41.0	30.0	41.0
30-31	36.68507845008719	40.0	36.5	41.0	30.0	41.0
32-33	36.547939516703195	40.0	36.0	41.0	30.0	41.0
34-35	36.39483163475546	40.0	36.0	41.0	30.0	41.0
36-37	36.209239324416345	40.0	35.5	41.0	29.0	41.0
38-39	36.057018847585766	40.0	35.0	41.0	28.0	41.0
40-41	35.91341992977054	40.0	35.0	41.0	27.0	41.0
42-43	35.74924016290937	40.0	35.0	41.0	27.0	41.0
44-45	35.556485913506044	40.0	35.0	41.0	26.0	41.0
46-47	35.249056412073244	40.0	35.0	41.0	24.5	41.0
48-49	34.96563160620137	39.0	35.0	41.0	23.5	41.0
50-51	34.74981143349447	39.0	35.0	41.0	23.0	41.0
52-53	34.54544783270327	39.0	34.5	41.0	22.0	41.0
54-55	34.31847664702503	39.0	34.0	41.0	21.5	41.0
56-57	34.06559357185564	38.0	34.0	41.0	20.0	41.0
58-59	33.80009514251926	38.0	34.0	40.0	19.5	41.0
60-61	33.4812458948779	37.0	33.0	40.0	17.5	41.0
62-63	33.17010058516993	37.0	33.0	40.0	15.0	41.0
64-65	32.83452850064228	36.0	33.0	39.5	12.5	41.0
66-67	32.459925698942484	36.0	32.5	39.0	9.0	41.0
68-69	32.064289375273596	35.0	32.0	39.0	7.0	41.0
70-71	31.65071704373043	35.0	32.0	38.0	3.5	40.0
72-73	31.262870773494708	35.0	31.5	37.0	2.0	39.5
74-75	30.949444158441942	35.0	31.0	37.0	2.0	39.0
76-77	30.662158885363276	35.0	31.0	36.0	2.0	39.0
78-79	30.373091625758654	35.0	31.0	36.0	2.0	37.5
80-81	30.346513096543408	35.0	31.0	35.5	2.0	37.0
82-83	30.160774805324202	35.0	31.0	35.0	2.0	37.0
84-85	29.97771024929304	35.0	31.0	35.0	2.0	36.0
86-87	29.823134438003827	35.0	31.0	35.0	2.0	36.0
88-89	29.663036092592293	35.0	31.0	35.0	2.0	36.0
90-91	29.524468888320662	35.0	31.0	35.0	2.0	35.0
92-93	29.42444781268519	35.0	31.0	35.0	2.0	35.0
94-95	29.32832233031564	35.0	31.0	35.0	2.0	35.0
96-97	29.273051665145168	35.0	31.0	35.0	2.0	35.0
98-99	29.18770308433238	35.0	30.5	35.0	2.0	35.0
100-101	29.096377596826116	35.0	30.0	35.0	2.0	35.0
102-103	27.793593611136394	33.0	28.0	34.5	2.0	35.0
104-105	28.791328735722484	34.0	29.5	35.0	2.0	35.0
106-107	28.952194566640742	34.0	30.0	35.0	2.0	35.0
108-109	28.94218080704576	35.0	30.0	35.0	2.0	35.0
110-111	28.84205775252898	35.0	30.0	35.0	2.0	35.0
112-113	28.709111661964936	34.0	30.0	35.0	2.0	35.0
114-115	28.595445245461278	34.0	30.0	35.0	2.0	35.0
116-117	28.457925968619964	34.0	29.5	35.0	2.0	35.0
118-119	28.278311698073015	34.0	29.0	35.0	2.0	35.0
120-121	28.3661589503276	35.0	30.0	35.0	2.0	35.0
122-123	28.231733599435266	35.0	30.0	35.0	2.0	35.0
124-125	27.53355747284246	34.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44180.0
3	17843.0
4	7682.0
5	7118.0
6	8672.0
7	10192.0
8	10892.0
9	12080.0
10	13840.0
11	16067.0
12	17638.0
13	21246.0
14	27455.0
15	25050.0
16	14244.0
17	12129.0
18	11568.0
19	12395.0
20	12252.0
21	14090.0
22	13917.0
23	12678.0
24	15872.0
25	20654.0
26	25276.0
27	31025.0
28	36934.0
29	44748.0
30	53796.0
31	65247.0
32	81568.0
33	105968.0
34	145230.0
35	220668.0
36	428001.0
37	759678.0
38	269236.0
39	478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.851826237237375	18.471591087509793	12.462360494058832	25.214222181194003
2	16.852265412754484	20.71255564184932	37.12512578910946	25.310053156286738
3	19.21040396101083	24.269991732156623	29.802421582961518	26.71718272387103
4	13.567468504273084	16.47228217391337	35.040464558667	34.919784763146545
5	13.870682113291302	37.27024570542204	34.05668309594657	14.802389085340092
6	33.09933903003811	35.720954705829435	16.32579929888649	14.853906965245969
7	28.300506305780893	32.18664114236847	20.58716210397173	18.92569044787891
8	26.99479246543105	33.86899496018234	19.800379100659608	19.335833473726996
9	26.94817361084879	14.448003626336266	18.954330827635705	39.649491935179235
10-11	25.657836090936698	25.221777940281054	27.318554936263627	21.801831032518617
12-13	26.371809078150115	22.914765776670837	27.4540485911395	23.259376554039545
14-15	23.858207991127518	24.220919084677668	25.09275537286111	26.82811755133371
16-17	23.334170070752478	27.02556091155648	25.8951532126656	23.74511580502545
18-19	23.3514969288488	25.507215004761903	28.2488677030645	22.8924203633248
20-21	24.250768234567875	24.735094133763106	27.91273671333876	23.101400918330263
22-23	23.7899367072587	24.84482832226444	27.814766442493067	23.550468527983796
24-25	23.445100354191265	25.045175914994093	28.168802833530105	23.340920897284533
26-27	23.233620119876484	25.040853245847288	28.216497741100266	23.509028893175966
28-29	23.14203167928946	24.891069976454457	28.42061618871948	23.546282155536602
30-31	23.418269687580224	24.901172256563186	28.183025939555407	23.49753211630118
32-33	23.12425963520263	25.190830177623326	28.11749422867282	23.567415958501226
34-35	23.31483952206365	25.31249982290748	28.17616616133119	23.196494493697678
36-37	23.60365598417498	24.98719279713844	27.856554038840205	23.552597179846373
38-39	23.55433289769221	24.921851875847757	27.98699234413772	23.53682288232231
40-41	23.48092833369508	24.931414313086222	28.12728158507128	23.460375768147415
42-43	23.73360156404953	24.703047818736483	27.94608940394224	23.617261213271753
44-45	23.40151007953912	24.979522039876862	27.76165316834092	23.8573147122431
46-47	23.6184436712041	24.93830448794236	27.427357595952955	24.015894244900583
48-49	23.57333686929703	25.029220811110786	27.290348707975486	24.107093611616698
50-51	23.268253174962044	25.440966425997008	27.088274880940105	24.202505518100846
52-53	23.4773560454426	25.380241907989436	26.969357956167045	24.173044090400914
54-55	23.23660653572648	25.504795414776556	26.87126574168377	24.387332307813196
56-57	23.29904302695564	25.59726292034492	26.59669590635543	24.506998146344007
58-59	22.970307763906643	25.490532724091352	26.902200191596933	24.636959320405076
60-61	23.242344361864568	25.582856780200775	26.678097897575263	24.496700960359394
62-63	22.890566801802958	25.694581906041243	26.727539698489426	24.687311593666376
64-65	22.67781679066013	25.646859385310027	26.771801840416508	24.90352198361333
66-67	22.77544740008995	25.688773153897927	26.69257229811297	24.843207147899157
68-69	23.069199097427713	25.30328461210962	26.645734333521844	24.981781956940818
70-71	22.721270808925674	25.072037617104808	26.812765666457995	25.393925907511523
72-73	22.696036287543	25.01495703124041	26.82627382239	25.46273285882659
74-75	22.531175037304273	25.171391874279887	26.766201386396695	25.531231702019152
76-77	22.367034376680913	24.862883096318768	26.930836420667102	25.839246106333214
78-79	22.35039557244443	24.992317468819877	26.797745349447982	25.85954160928771
80-81	22.292903521191672	25.022269047874484	26.691918772184053	25.99290865874979
82-83	22.03677152083657	24.861432967743653	26.830600587152126	26.27119492426765
84-85	21.995658442210686	24.790396971297856	26.728591063825206	26.485353522666248
86-87	21.863393383110502	24.679285537153167	26.86135076019002	26.595970319546304
88-89	21.82666260932211	24.70410945914728	26.712891683444766	26.756336248085844
90-91	21.69873083176683	24.628062988461284	26.74187635620727	26.93132982356461
92-93	21.561268144342556	24.50240350562392	26.828298091361546	27.108030258671977
94-95	21.185340256386674	24.434247015958395	27.00478092600718	27.37563180164776
96-97	21.23826691139151	24.292756344835365	26.938886222403053	27.530090521370077
98-99	21.144058376115375	24.324476967965936	26.83784897214494	27.693615683773753
100-101	21.084318459161196	24.37913460049039	26.803450868384164	27.733096071964248
102-103	20.80053750218639	24.269047270952406	26.870708208619455	28.059707018241753
104-105	20.616300696142627	24.35246949595058	26.818518447864665	28.212711360042125
106-107	20.394511667617405	24.498105452443646	26.922087440075256	28.1852954398637
108-109	20.235535826629555	24.59297128365968	26.876219036911024	28.295273852799742
110-111	20.199225109753453	24.705402111973548	26.76350244648994	28.33187033178306
112-113	20.029858888043524	24.90642653633121	26.65606519996464	28.407649375660625
114-115	19.812790242835522	24.981107714077787	26.695475238497995	28.510626804588696
116-117	19.57175275587131	24.955572700890812	26.849576125131147	28.623098418106736
118-119	19.56779910704168	24.99482507233567	26.79092159040259	28.646454230220066
120-121	19.478682661351474	25.11485816171417	26.74554471956516	28.660914457369195
122-123	19.374098192229532	25.238568288095003	26.596001450446842	28.79133206922862
124-125	19.113437340066515	25.358109371370514	26.611043649212395	28.917409639350577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	177.5
2	243.5
3	485.0
4	398.5
5	250.5
6	454.0
7	980.0
8	1578.5
9	1706.0
10	1285.5
11	933.0
12	907.0
13	987.0
14	1119.5
15	1301.0
16	1519.0
17	1696.0
18	1905.5
19	2157.5
20	2461.5
21	2902.0
22	3438.0
23	4128.5
24	4903.5
25	5806.5
26	7561.5
27	9947.0
28	11920.5
29	14519.0
30	17384.0
31	20223.5
32	24194.0
33	28486.0
34	32373.0
35	36456.0
36	41594.0
37	46398.0
38	50528.0
39	54614.5
40	59667.0
41	65236.5
42	70657.5
43	77307.5
44	85108.0
45	92934.5
46	99781.5
47	106656.5
48	113084.0
49	117761.0
50	123481.5
51	125705.0
52	121655.5
53	118845.0
54	116066.5
55	110309.0
56	101825.0
57	91025.0
58	82254.5
59	72862.0
60	60527.5
61	49133.5
62	39086.5
63	29366.5
64	20882.0
65	14966.0
66	10899.5
67	7917.0
68	5768.5
69	4201.5
70	3097.5
71	2318.5
72	1835.5
73	1574.5
74	1188.5
75	872.0
76	613.0
77	374.0
78	228.5
79	131.5
80	87.5
81	57.0
82	41.5
83	32.0
84	17.5
85	11.0
86	11.5
87	10.0
88	8.0
89	10.5
90	11.0
91	8.0
92	9.0
93	7.5
94	5.0
95	7.0
96	9.0
97	7.5
98	5.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16769860481559384
2	0.012048615976615865
3	0.0
4	0.003965845384152557
5	0.14163733514830562
6	0.0793546776390907
7	0.007365141427711892
8	0.01091551729542942
9	0.011595376504141287
10-11	0.005250023889497195
12-13	0.010292313020776876
14-15	0.01212415588869496
16-17	0.01588226651463
18-19	0.020924555645909684
20-21	0.023757302348875797
22-23	0.026438969227683715
24-25	0.027647607820949258
26-27	0.023889497195014212
28-29	0.023020788206104606
30-31	0.026571164073822134
32-33	0.02589130486511027
34-35	0.02589130486511027
36-37	0.024890401030062244
38-39	0.020792360799771266
40-41	0.027779802667087676
42-43	0.02387061221699444
44-45	0.032444392237971874
46-47	0.027836457601147
48-49	0.019678147096604592
50-51	0.015485681976214747
52-53	0.01675097550353961
54-55	0.018696128239576344
56-57	0.015334602152056556
58-59	0.014465893163146946
60-61	0.01786518920670628
62-63	0.015221292283937912
64-65	0.028724051568076378
66-67	0.02128337022828539
68-69	0.019111597756011373
70-71	0.027420988084711967
72-73	0.0324255072599521
74-75	0.01726086991007351
76-77	0.008724859845135626
78-79	0.013880458844533951
80-81	0.01642993087720345
82-83	0.0159578064267091
84-85	0.01512686739383904
86-87	0.01040562288889552
88-89	0.020981210579969007
90-91	0.019602607184525497
92-93	0.01631662100908481
94-95	0.011954191086516994
96-97	0.02086790071185036
98-99	0.021358910140364486
100-101	0.027836457601147
102-103	0.020697935909672394
104-105	0.014220388448889886
106-107	0.020282466393237365
108-109	0.010632242625132808
110-111	0.024607126359765627
112-113	0.018469508503339054
114-115	0.013691609064336209
116-117	0.011500951614042416
118-119	0.008573780020977433
120-121	0.003871420494053687
122-123	0.005552183537813581
124-125	8.309390328700596E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2647607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.82909898457975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.15344034706978	27.671388044480743
2	14.479649222072762	12.113413611441496
3	6.692061880997803	8.397687564935701
4	3.6133507191375878	6.045728195872344
5	2.2789595691667723	4.766341270026607
6	1.5098054257201048	3.7892280359941104
7	1.0645596430654056	3.117069947934022
8	0.7398176076559346	2.4756723152940063
9	0.5552544255755968	2.090321309612485
>10	2.720879408503536	19.740114253139556
>50	0.1356033254868296	3.8535924884458757
>100	0.05362617543477156	3.7344858899445943
>500	0.0018134849172227229	0.5301985881452712
>1k	9.06742458608441E-4	0.8780382942652329
>5k	2.7202273758253234E-4	0.7967201904680554
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7914	0.29891143209698423	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6568	0.24807307126775233	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	6524	0.2464111932020122	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4795	0.18110693920963344	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	4154	0.15689639738828307	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	2850	0.10764437471271228	RNA PCR Primer, Index 39 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.7769956039548164E-5	0.0	0.0	0.0	0.0
6	3.7769956039548164E-5	0.0	0.0	0.0	0.0
7	7.553991207909633E-5	0.0	0.0	0.0	0.0
8	7.553991207909633E-5	0.0	0.0	0.0	0.0
9	7.553991207909633E-5	0.0	0.0	3.7769956039548164E-5	0.0
10-11	1.133098681186445E-4	0.0	0.0	9.442489009887041E-5	0.0
12-13	1.133098681186445E-4	0.0	0.0	1.133098681186445E-4	0.0
14-15	1.5107982415819265E-4	0.0	0.0	1.5107982415819265E-4	0.0
16-17	1.5107982415819265E-4	0.0	0.0	1.6996480217796673E-4	0.0
18-19	2.0773475821751492E-4	0.0	0.0	2.0773475821751492E-4	0.0
20-21	2.26619736237289E-4	0.0	0.0	3.021596483163853E-4	0.0
22-23	2.26619736237289E-4	0.0	0.0	5.665493405932224E-4	0.0
24-25	3.399296043559335E-4	0.0	0.0	0.001114213703166671	0.0
26-27	3.399296043559335E-4	0.0	0.0	0.002492817098610179	0.0
28-29	3.399296043559335E-4	0.0	0.0	0.008781514779194949	0.0
30-31	3.399296043559335E-4	0.0	0.0	0.027269908260553775	0.0
32-33	3.399296043559335E-4	0.0	0.0	0.07357587436503982	0.0
34-35	3.399296043559335E-4	0.0	0.0	0.15296832196017007	0.0
36-37	4.1546951643502985E-4	0.0	0.0	0.2931892837569926	0.0
38-39	4.1546951643502985E-4	0.0	0.0	0.5135392072917166	0.0
40-41	4.53239472474578E-4	0.0	0.0	0.7598937455596695	0.0
42-43	5.665493405932224E-4	0.0	0.0	0.9878354302583428	0.0
44-45	6.79859208711867E-4	0.0	0.0	1.2657278818193183	0.0
46-47	8.120540548502855E-4	0.0	0.0	1.5970081662421953	0.0
48-49	8.309390328700597E-4	0.0	0.0	1.9430376184985159	0.0
50-51	8.309390328700597E-4	0.0	0.0	2.3082542084229267	0.0
52-53	8.498240108898337E-4	0.0	0.0	2.6650480981505185	0.0
54-55	8.687089889096078E-4	0.0	0.0	3.05947974907152	0.0
56-57	9.06478944949156E-4	0.0	0.0	3.5242768280942	0.0
58-59	9.442489009887042E-4	0.0	0.0	4.029695494837413	0.0
60-61	9.442489009887042E-4	0.0	0.0	4.564612497247515	0.0
62-63	9.442489009887042E-4	0.0	0.0	5.08657440473605	0.0
64-65	9.442489009887042E-4	0.0	0.0	5.641603908737211	0.0
66-67	0.0010197888130678006	0.0	0.0	6.287470157013484	0.0
68-69	0.0010575587691073487	0.0	0.0	7.005004896874801	0.0
70-71	0.001114213703166671	0.0	0.0	7.795360111980365	0.0
72-73	0.001133098681186445	0.0	0.0	8.565546170560811	0.0
74-75	0.001133098681186445	0.0	0.0	9.322399434659298	0.0
76-77	0.001133098681186445	0.0	0.0	10.169957248186758	0.0
78-79	0.001133098681186445	0.0	0.0	11.116623426361993	0.0
80-81	0.001189753615245767	0.0	0.0	12.106536204202513	0.0
82-83	0.0012086385932655412	0.0	0.0	13.13912903236772	0.0
84-85	0.0013030634833644116	0.0	0.0	14.151571588985828	0.0
86-87	0.0014352583295028304	0.0	0.0	15.204106953939917	0.0
88-89	0.0014730282855423785	0.0	0.0	16.333409754544387	0.0
90-91	0.001586338153661023	0.0	0.0	17.528337853767574	0.0
92-93	0.0016996480217796675	0.0	0.0	18.73716529681331	0.0
94-95	0.0016996480217796675	0.0	0.0	19.937362304904013	0.0
96-97	0.0016996480217796675	0.0	0.0	21.133329077918283	0.0
98-99	0.0017185329997994416	0.0	0.0	22.398169365770677	0.0
100-101	0.0017940729118785378	0.0	0.0	23.742345446284133	0.0
102-103	0.00185072784593786	0.0	0.0	25.095737396071243	0.0
104-105	0.002039577626135601	0.0	0.0	26.404617452665747	0.0
106-107	0.0020962325601949234	0.0	0.0	27.690684455812363	0.0
108-109	0.0021151175382146973	0.0	0.0	29.01992251871218	0.0
110-111	0.0021906574502937935	0.0	0.0	30.390084328980848	0.0
112-113	0.0021906574502937935	0.0	0.0	31.77422102298415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7680	0.0	42.7237	1
GTATCAA	11215	0.0	38.300163	1
TCAACGC	14095	0.0	30.359245	4
ATCAACG	14255	0.0	30.185722	3
CAACGCA	14220	0.0	30.092375	5
TATCAAC	14440	0.0	29.75772	2
AACGCAG	14600	0.0	29.435108	6
ACGCAGA	16510	0.0	25.762861	7
TACATGG	12150	0.0	25.359802	2
GTACATG	12440	0.0	25.177137	1
CATGGGG	7595	0.0	24.95347	4
CGCAGAG	17095	0.0	24.742981	8
GAGTACT	9760	0.0	24.290142	12-13
AGTACTT	10035	0.0	23.59485	12-13
ACATGGG	13150	0.0	23.11405	3
GCAGAGT	18065	0.0	22.721985	9
GTACTTT	10620	0.0	22.239958	14-15
GTACCGT	545	0.0	21.843126	6
AGAGTAC	16395	0.0	21.154188	10-11
TACCGTA	425	1.8189894E-12	20.996044	7
>>END_MODULE