FastQCFastQC Report
Mon 15 Aug 2016
SRR2921088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868887
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99300.030.1483651
TACATGG149000.029.041512
GTACATG154050.028.5695881
CATGGGG88850.028.5383474
ACATGGG151300.027.7742563
AACGCAG105400.027.1596976
GGTATCA73600.027.0631281
TCAACGC108350.026.4236494
CAACGCA111150.025.7044565
TATCAAC117850.025.3034172
ATCAACG114050.025.2074243
ACGCAGA120000.023.854037
CGCAGAG121550.023.4519258
ATGGGGG46800.019.967815
GCAGAGT137400.019.9233999
GTATAGC15250.019.5528241
ATGGGGA51300.018.912415
GTATTAG13300.018.8324571
GTATAGG9600.018.0150761
AGAGTAC120750.017.39440710-11
Produced by FastQC (version 0.11.5)