##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3868887
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.157671702481878	31.0	31.0	34.0	26.0	34.0
2	30.2573499820491	31.0	31.0	34.0	26.0	34.0
3	30.35170967774453	31.0	31.0	34.0	26.0	34.0
4	33.72277660215974	37.0	35.0	37.0	28.0	37.0
5	33.80239562437466	37.0	35.0	37.0	28.0	37.0
6	33.7957976027731	37.0	35.0	37.0	29.0	37.0
7	33.67275627331581	37.0	35.0	37.0	28.0	37.0
8	33.67120311345356	37.0	35.0	37.0	28.0	37.0
9	35.135578268375376	39.0	35.0	39.0	28.0	39.0
10-11	35.10927987816651	39.0	35.0	39.0	27.5	39.0
12-13	35.06103938936444	39.0	35.0	39.0	27.0	39.0
14-15	36.321327554927294	40.0	37.0	41.0	27.0	41.0
16-17	36.31917034537323	40.0	37.0	41.0	27.0	41.0
18-19	36.35549565029943	40.0	37.0	41.0	27.0	41.0
20-21	36.399183150089414	40.0	37.0	41.0	27.0	41.0
22-23	36.3494046220528	40.0	37.0	41.0	27.0	41.0
24-25	36.35015884413269	40.0	36.5	41.0	28.0	41.0
26-27	36.30198814284314	40.0	36.0	41.0	28.0	41.0
28-29	36.17826056951263	40.0	36.0	41.0	27.5	41.0
30-31	36.08144667962647	40.0	36.0	41.0	27.0	41.0
32-33	35.95778346589084	40.0	36.0	41.0	27.0	41.0
34-35	35.84716379671983	40.0	36.0	41.0	27.0	41.0
36-37	35.72833207586575	40.0	35.5	41.0	26.5	41.0
38-39	35.597430733955264	40.0	35.0	41.0	26.0	41.0
40-41	35.46783493547369	40.0	35.0	41.0	25.5	41.0
42-43	35.29408535322949	40.0	35.0	41.0	25.0	41.0
44-45	35.12388537065053	40.0	35.0	41.0	24.0	41.0
46-47	34.95154523768722	39.0	35.0	41.0	24.0	41.0
48-49	34.736254121663414	39.0	34.5	41.0	23.0	41.0
50-51	34.56976114836127	39.0	34.0	41.0	23.0	41.0
52-53	34.40119173808901	39.0	34.0	41.0	22.5	41.0
54-55	34.18017287659215	38.5	34.0	40.5	21.5	41.0
56-57	33.945431076172554	38.0	33.0	40.0	20.0	41.0
58-59	33.68505890712238	38.0	33.0	40.0	19.5	41.0
60-61	33.39513805908521	37.5	33.0	40.0	18.0	41.0
62-63	33.11252887458331	37.0	32.5	40.0	17.0	41.0
64-65	32.80672981661134	36.5	32.0	40.0	13.5	41.0
66-67	32.46777755463005	36.0	32.0	39.0	12.5	41.0
68-69	32.10124824012694	35.5	31.5	39.0	9.0	40.5
70-71	31.738356664332663	35.0	31.0	38.5	8.0	40.0
72-73	31.374260737002658	35.0	31.0	37.5	7.0	40.0
74-75	31.307189380304983	35.0	31.0	37.0	7.0	39.0
76-77	31.321542086910267	35.0	32.0	37.0	6.5	39.0
78-79	31.114149624943813	35.0	32.0	36.5	2.0	39.0
80-81	30.87412865767338	35.0	32.0	36.0	2.0	38.0
82-83	30.604061840007216	35.0	32.0	36.0	2.0	37.0
84-85	30.330677401536928	35.0	31.5	35.0	2.0	37.0
86-87	30.15959305609081	35.0	31.0	35.0	2.0	36.0
88-89	29.96535476481996	35.0	31.0	35.0	2.0	36.0
90-91	29.806860345107005	35.0	31.0	35.0	2.0	36.0
92-93	29.681292319987634	35.0	31.0	35.0	2.0	35.5
94-95	29.55347132133867	35.0	31.0	35.0	2.0	35.0
96-97	29.448308130994782	35.0	31.0	35.0	2.0	35.0
98-99	29.340771389807973	35.0	31.0	35.0	2.0	35.0
100-101	29.20276270669058	35.0	30.5	35.0	2.0	35.0
102-103	27.938882681246568	33.0	28.0	34.5	2.0	35.0
104-105	28.86404759301577	34.0	29.5	35.0	2.0	35.0
106-107	28.98565582297958	35.0	30.0	35.0	2.0	35.0
108-109	28.913773909654118	35.0	30.0	35.0	2.0	35.0
110-111	28.811663406039	35.0	30.0	35.0	2.0	35.0
112-113	28.690787815720647	35.0	30.0	35.0	2.0	35.0
114-115	28.5467282967944	35.0	30.0	35.0	2.0	35.0
116-117	28.401523745718084	35.0	29.0	35.0	2.0	35.0
118-119	28.200555224280265	34.0	29.0	35.0	2.0	35.0
120-121	27.993339557345564	34.0	29.0	35.0	2.0	35.0
122-123	27.699665433495475	34.0	29.0	35.0	2.0	35.0
124-125	26.460748918228937	33.0	24.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121119.0
3	17708.0
4	11602.0
5	12194.0
6	17927.0
7	18939.0
8	16919.0
9	17882.0
10	19152.0
11	20406.0
12	21368.0
13	21027.0
14	22178.0
15	19791.0
16	16083.0
17	14758.0
18	13589.0
19	13650.0
20	14011.0
21	15096.0
22	17669.0
23	22795.0
24	31287.0
25	40533.0
26	45747.0
27	48988.0
28	56463.0
29	67620.0
30	79144.0
31	97274.0
32	122222.0
33	158007.0
34	218242.0
35	330128.0
36	614776.0
37	1058794.0
38	413100.0
39	699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.757728594438596	18.185765171415046	12.043325645304522	25.013180588841834
2	16.154114954325706	20.700283147779757	38.39638568209077	24.749216215803767
3	18.831385349078623	24.86200107558841	29.363744703218565	26.9428688721144
4	12.691179993367133	16.781983020479625	36.59865821536539	33.92817877078786
5	13.638034694074491	38.27457376209844	34.18417492589782	13.903216617929248
6	32.519066782262136	36.85136061903961	17.08199945652877	13.547573142169487
7	27.830371400247948	33.10978779605422	21.12887003022613	17.9309707734717
8	25.89297659035963	35.61762796353181	19.930682188391362	18.558713257717198
9	26.19477316661017	16.180747304161955	19.145586899533935	38.47889262969394
10-11	24.71597402961971	26.611865088456415	27.858005060935753	20.814155820988123
12-13	25.274651218531346	24.383316127682015	27.456830544405264	22.885202109381378
14-15	22.821735217539622	25.7886990829455	24.84318511601048	26.5463805835044
16-17	22.95286234530857	28.206488181919436	25.776650819188816	23.063998653583173
18-19	22.912739159173842	27.4617144823322	27.101166384028417	22.524379974465543
20-21	24.060355882860467	26.731637541836196	26.631414355095874	22.576592220207466
22-23	23.474519398712154	26.65358878147141	26.392444934692698	23.479446885123735
24-25	23.19376428222102	26.775315562144726	26.653018941554112	23.377901214080136
26-27	23.13843934404514	26.8811330185887	26.594364918874952	23.386062718491214
28-29	23.122404305565595	27.100980476743057	26.637446866966823	23.13916835072452
30-31	23.391517790795422	27.032781514216975	26.43828261449653	23.13741808049108
32-33	22.937984467043727	27.071051134779744	26.436657456001406	23.554306942175124
34-35	23.29475142863167	27.072154581833473	26.52088041920585	23.11221357032901
36-37	23.447627062366035	26.897005518182866	26.377912677902575	23.277454741548524
38-39	23.158223931991824	27.05379721295599	26.446265385857014	23.341713469195177
40-41	23.348235068589332	27.024097038533974	26.602604856609226	23.025063036267472
42-43	23.25759761769357	26.964417893321063	26.471018475058482	23.306966013926882
44-45	23.24030625165062	27.082471648131868	26.42020594567316	23.257016154544356
46-47	23.200403376871705	27.049411726974693	26.306038018451204	23.444146877702394
48-49	23.10020218333168	27.04748450259719	26.40859086468615	23.44372244938498
50-51	23.028143203478564	27.24989531039724	26.29458813597155	23.427373350152646
52-53	23.199773082434042	26.99251963605237	26.192254950628875	23.615452330884718
54-55	23.099688425165642	26.949360552411523	26.293071780177797	23.657879242245038
56-57	23.075320013003225	27.066115301571497	26.1587741574195	23.69979052800577
58-59	23.03071222870214	26.717858240687733	26.38026956142439	23.871159969185737
60-61	23.00043562704944	26.78892938185453	26.469206548271305	23.741428442824724
62-63	22.85183476347524	26.70541172215783	26.571911115319253	23.870842399047685
64-65	22.84988263997711	26.600669653134783	26.514867758508924	24.03457994837918
66-67	22.89288869455029	26.778518273367936	26.347709949381866	23.98088308269991
68-69	23.046425951391324	26.4579084549378	26.507432332456933	23.988233261213946
70-71	22.78630225289987	26.296127487220183	26.719707014890858	24.197863244989087
72-73	22.852659367772578	26.355572481277	26.703542612407517	24.088225538542908
74-75	22.77114380753846	26.386200548550782	26.666736516086836	24.17591912782392
76-77	22.683600932295274	26.139193889010915	26.991702323675348	24.18550285501846
78-79	22.78804259191935	26.19976495319434	26.934043724603363	24.07814873028295
80-81	22.716338131793023	26.137627129518055	26.974468807252585	24.171565931436334
82-83	22.665374859166683	26.124448146821077	27.00467094480886	24.20550604920338
84-85	22.706931621379827	26.115966670926888	26.8889898463578	24.28811186133549
86-87	22.5699389543009	25.965627793390116	27.019211604179876	24.44522164812911
88-89	22.661005201099705	25.88515682182031	26.96406818587401	24.48976979120598
90-91	22.562190534828254	25.994725187166605	26.947386393621592	24.495697884383553
92-93	22.64129984281518	25.85641803532553	27.003097788873177	24.49918433298612
94-95	22.41001937818294	25.79966857499277	27.15848264727115	24.631829399553144
96-97	22.427110091191363	25.684685368653952	27.16209744142442	24.726107098730264
98-99	22.384109745937305	25.75598931531135	27.02384552052938	24.83605541822196
100-101	22.287176694518156	25.946499010139203	27.09541016326074	24.670914132081904
102-103	22.167705229600926	25.95035717883486	27.142229218536084	24.739708373028133
104-105	22.007819175330606	26.045708978265715	26.987819896957387	24.958651949446296
106-107	21.861553026162902	26.108926058143894	27.061796563240154	24.967724352453047
108-109	21.897281937521935	26.081695678365648	27.108211414431732	24.912810969680688
110-111	21.912087676134632	26.30058973580565	26.996067436295196	24.791255151764517
112-113	21.888103060900892	26.350379954816184	27.00057630834669	24.76094067593623
114-115	21.76129504348205	26.396879738700235	26.989825622945663	24.851999594872055
116-117	21.698789082927014	26.336932201138396	27.05784828724064	24.906430428693955
118-119	21.7720409025038	26.219169702193923	26.98401263831237	25.0247767569899
120-121	21.761113856710747	26.350546086830523	27.03921578774416	24.84912426871457
122-123	21.794590712770777	26.332657751259813	26.967676443916016	24.905075092053394
124-125	21.801947726412685	26.413887011168214	26.86591996274027	24.91824529967883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2592.0
1	1843.0
2	943.0
3	697.0
4	559.5
5	473.0
6	431.0
7	486.0
8	578.0
9	591.5
10	573.0
11	595.5
12	655.5
13	783.5
14	927.0
15	1029.5
16	1227.5
17	1495.0
18	1834.5
19	2302.0
20	2824.0
21	3523.0
22	4557.5
23	6006.0
24	7840.5
25	10315.0
26	14129.0
27	18804.0
28	23240.5
29	28797.0
30	34876.5
31	41157.0
32	48772.0
33	55249.5
34	61805.5
35	69080.5
36	77781.0
37	87119.0
38	94702.5
39	101131.5
40	107379.5
41	113576.5
42	120166.5
43	128389.0
44	136034.5
45	142491.5
46	148565.0
47	152654.5
48	156239.0
49	159987.5
50	162993.0
51	161512.5
52	154914.0
53	149744.5
54	143130.5
55	132413.0
56	121157.5
57	109809.0
58	100727.0
59	91003.0
60	77125.0
61	64259.0
62	53077.5
63	40330.5
64	29516.0
65	22998.0
66	17639.0
67	13397.5
68	10251.5
69	7684.5
70	6068.5
71	4870.5
72	3871.0
73	3430.5
74	2810.0
75	2171.0
76	1673.5
77	1160.5
78	779.5
79	500.5
80	317.0
81	211.0
82	147.5
83	111.0
84	86.5
85	80.0
86	69.5
87	50.0
88	39.0
89	28.5
90	18.0
91	20.5
92	27.0
93	20.0
94	13.0
95	13.0
96	13.0
97	13.5
98	12.5
99	13.0
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28279450911851395
2	0.06999429034758575
3	0.08002301437079966
4	0.08501152915554266
5	0.09346357234005542
6	0.1250230363409425
7	0.1124354368581972
8	0.11290068694174836
9	0.11881970189359368
10-11	0.09799976065467925
12-13	0.0877125643628258
14-15	0.09668155208461762
16-17	0.09856839964568621
18-19	0.0910339330148438
20-21	0.09082715519993217
22-23	0.0728504089160526
24-25	0.08492106386151883
26-27	0.0967461701517775
28-29	0.08972864805821415
30-31	0.08474013327347116
32-33	0.09360573208780716
34-35	0.08755748100164207
36-37	0.0877125643628258
38-39	0.07751583336499619
40-41	0.089263397974663
42-43	0.07892450722908165
44-45	0.07537051353528805
46-47	0.06532886589864216
48-49	0.06704770648509507
50-51	0.06968412362521831
52-53	0.0818323202512764
54-55	0.08692422394347522
56-57	0.09544088519514786
58-59	0.06450175463899566
60-61	0.08258188983033105
62-63	0.08688545310317929
64-65	0.0776709167261799
66-67	0.07191990874895028
68-69	0.07948022260665664
70-71	0.07818786126345897
72-73	0.05995264271093986
74-75	0.08863014091649614
76-77	0.09273984998786472
78-79	0.07362582572197121
80-81	0.08635558495246824
82-83	0.09051698847756473
84-85	0.09493686427130076
86-87	0.08210371613334791
88-89	0.06195580279289625
90-91	0.08651066831365196
92-93	0.0625373653973352
94-95	0.08936678688211881
96-97	0.07768384033961188
98-99	0.07221715185788574
100-101	0.08903077293288743
102-103	0.06220135144810381
104-105	0.06778435245071775
106-107	0.08675621696885952
108-109	0.09249430133265718
110-111	0.08639435579276418
112-113	0.07498280513232876
114-115	0.08895323125229557
116-117	0.08220710504080372
118-119	0.07403938135179446
120-121	0.10641303299889605
122-123	0.08611003629726069
124-125	0.1066068872003757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3868887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.35976350256839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7087512997843	32.066704486120045
2	14.673644591901741	13.898806751864164
3	6.537618099798545	9.28860141229709
4	3.480237683233494	6.592929344426593
5	2.111472437302844	4.999941763642719
6	1.3380395608408318	3.802154229510136
7	0.9185875911662438	3.04528637518191
8	0.6674560399188803	2.5288448159135215
9	0.471340144911255	2.009030201303702
>10	1.9743117530602237	15.190210117878634
>50	0.07974841002158423	2.5677609468834373
>100	0.03649184617349582	3.0138721148116057
>500	0.0018075686428679003	0.583677426780802
>1k	4.929732436646828E-4	0.4121800133855359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8093058804767367E-4
2	0.0	0.0	0.0	0.0	1.8093058804767367E-4
3	0.0	0.0	0.0	0.0	1.8093058804767367E-4
4	0.0	0.0	0.0	0.0	1.8093058804767367E-4
5	0.0	0.0	0.0	0.0	2.3262504177558042E-4
6	0.0	0.0	0.0	0.0	3.1016672236744056E-4
7	0.0	0.0	0.0	0.0	3.1016672236744056E-4
8	0.0	0.0	0.0	0.0	3.1016672236744056E-4
9	0.0	0.0	0.0	0.0	3.360139492313939E-4
10-11	0.0	0.0	0.0	5.169445372790676E-5	3.6186117609534734E-4
12-13	0.0	0.0	0.0	5.169445372790676E-5	9.305001671023217E-4
14-15	0.0	0.0	0.0	5.169445372790676E-5	0.0010726599148540652
16-17	0.0	0.0	0.0	7.754168059186014E-5	0.0012148196626058088
18-19	1.292361343197669E-5	0.0	0.0	1.292361343197669E-4	0.0012406668894697622
20-21	2.584722686395338E-5	0.0	0.0	1.5508336118372028E-4	0.0015508336118372028
22-23	2.584722686395338E-5	0.0	0.0	3.2309033579941724E-4	0.00175761142674883
24-25	2.584722686395338E-5	0.0	0.0	8.141876462145315E-4	0.0018868475610685966
26-27	2.584722686395338E-5	0.0	0.0	0.001628375292429063	0.002132396216276154
28-29	2.584722686395338E-5	0.0	0.0	0.004342334113144167	0.0021711670565720837
30-31	2.584722686395338E-5	0.0	0.0	0.015624648639259818	0.0022228615102999907
32-33	2.584722686395338E-5	0.0	0.0	0.04313902163593819	0.0025330282326674314
34-35	2.584722686395338E-5	0.0	0.0	0.0866011336076758	0.002713958820715105
36-37	2.584722686395338E-5	0.0	0.0	0.15885705630585747	0.002713958820715105
38-39	2.584722686395338E-5	0.0	0.0	0.27461386181607267	0.0028690421818988255
40-41	2.584722686395338E-5	0.0	0.0	0.40529744084022096	0.002920736635626732
42-43	2.584722686395338E-5	0.0	0.0	0.5320005469273205	0.0029724310893546385
44-45	2.584722686395338E-5	0.0	0.0	0.6905215892839465	0.003114590837106382
46-47	2.584722686395338E-5	0.0	0.0	0.8833806725293347	0.0032567505848581256
48-49	2.584722686395338E-5	0.0	0.0	1.0842394724891165	0.0033472158788819626
50-51	2.584722686395338E-5	0.0	0.0	1.2992496291569124	0.003411833946041846
52-53	2.584722686395338E-5	0.0	0.0	1.51354898708595	0.003579840920657543
54-55	2.584722686395338E-5	0.0	0.0	1.7576243503622617	0.0036961534415453335
56-57	2.584722686395338E-5	0.0	0.0	2.054208355012695	0.0037220006684092867
58-59	2.584722686395338E-5	0.0	0.0	2.3811628512282734	0.00374784789527324
60-61	2.584722686395338E-5	0.0	0.0	2.7302038027990996	0.0037736951221371937
62-63	2.584722686395338E-5	0.0	0.0	3.0788311987401027	0.004148479911664517
64-65	2.584722686395338E-5	0.0	0.0	3.454572335661393	0.004277716045984285
66-67	2.584722686395338E-5	0.0	0.0	3.8899688721846877	0.004342334113144167
68-69	2.584722686395338E-5	0.0	0.0	4.383482898311581	0.004406952180304052
70-71	2.584722686395338E-5	0.0	0.0	4.929970815896148	0.004484493860895911
72-73	2.584722686395338E-5	0.0	0.0	5.477492622555273	0.005040209238470909
74-75	2.584722686395338E-5	0.0	0.0	6.035999500631577	0.005195292599654629
76-77	2.584722686395338E-5	0.0	0.0	6.653399285117399	0.005221139826518582
78-79	2.584722686395338E-5	0.0	0.0	7.340638276589624	0.00527283428024649
80-81	2.584722686395338E-5	0.0	0.0	8.074621977845307	0.00538914680113428
82-83	2.584722686395338E-5	0.0	0.0	8.836649920248382	0.005557153775749977
84-85	2.584722686395338E-5	0.0	0.0	9.602593717521344	0.0055959246160459065
86-87	2.584722686395338E-5	0.0	0.0	10.40261708341443	0.00577685520409358
88-89	2.584722686395338E-5	0.0	0.0	11.271833475622316	0.005970709405573231
90-91	2.584722686395338E-5	0.0	0.0	12.192007675592489	0.005970709405573231
92-93	2.584722686395338E-5	0.0	0.0	13.143146853345678	0.006125792766756951
94-95	2.584722686395338E-5	0.0	0.0	14.081918133044464	0.006216258060780787
96-97	2.584722686395338E-5	0.0	0.0	15.045825840868446	0.006578119236876135
98-99	2.584722686395338E-5	0.0	0.0	16.051360507556826	0.006771973438355785
100-101	2.584722686395338E-5	0.0	0.0	17.14285012718128	0.006784897051787762
102-103	2.584722686395338E-5	0.0	0.0	18.243825679064805	0.007017522093563343
104-105	2.584722686395338E-5	0.0	0.0	19.32177135181255	0.007185529068179039
106-107	2.584722686395338E-5	0.0	0.0	20.40015642741698	0.007211376295042993
108-109	2.584722686395338E-5	0.0	0.0	21.493545818215935	0.007314765202498807
110-111	2.584722686395338E-5	1.292361343197669E-5	0.0	22.630061307037398	0.007392306883090667
112-113	2.584722686395338E-5	2.584722686395338E-5	0.0	23.793910238267493	0.007392306883090667
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9930	0.0	30.148365	1
TACATGG	14900	0.0	29.04151	2
GTACATG	15405	0.0	28.569588	1
CATGGGG	8885	0.0	28.538347	4
ACATGGG	15130	0.0	27.774256	3
AACGCAG	10540	0.0	27.159697	6
GGTATCA	7360	0.0	27.063128	1
TCAACGC	10835	0.0	26.423649	4
CAACGCA	11115	0.0	25.704456	5
TATCAAC	11785	0.0	25.303417	2
ATCAACG	11405	0.0	25.207424	3
ACGCAGA	12000	0.0	23.85403	7
CGCAGAG	12155	0.0	23.451925	8
ATGGGGG	4680	0.0	19.96781	5
GCAGAGT	13740	0.0	19.923399	9
GTATAGC	1525	0.0	19.552824	1
ATGGGGA	5130	0.0	18.91241	5
GTATTAG	1330	0.0	18.832457	1
GTATAGG	960	0.0	18.015076	1
AGAGTAC	12075	0.0	17.394407	10-11
>>END_MODULE