FastQCFastQC Report
Mon 15 Aug 2016
SRR2921088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868887
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.2126192881828805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73390.18969279795455385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.12437685566934367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.045.5917171
GTATCAA136000.042.4979971
TCAACGC169200.033.8477364
ATCAACG171500.033.4633033
CAACGCA172550.033.294645
TATCAAC173950.032.9919852
AACGCAG176800.032.7262346
ACGCAGA198550.028.9419167
CGCAGAG201200.028.4734958
GCAGAGT221700.025.3307939
GTACATG153050.024.6691361
TACATGG150350.024.376892
GAGTACT127700.023.5951412-13
ACATGGG150550.023.4340613
CATGGGG85300.023.2649354
AGAGTAC204400.023.04994810-11
AGTACTT140600.022.57268112-13
CAGAGTA212250.021.94520810-11
GTACTTT137500.021.87160114-15
TACTTTT165350.019.21317714-15
Produced by FastQC (version 0.11.5)