FastQCFastQC Report
Mon 15 Aug 2016
SRR2921087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5564621
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT113760.20443440802167837No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT72850.13091637328040848No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC69600.12507590364195514No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG67610.12149973915564061No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT57360.10307979644974923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.040.8942831
GTATCAA122450.040.3837241
CAACGCA144450.032.8848575
TCAACGC145550.032.5957264
TACATGG221000.032.5367052
AACGCAG148100.032.029016
CATGGGG157150.032.0078774
ATCAACG149050.031.9501253
TATCAAC154050.031.4529652
ACATGGG229400.030.8274483
GTATAAT64600.030.1020241
GTACATG256900.028.4434721
ACGCAGA168250.027.872777
CGCAGAG172250.027.1564088
ATAATGG73950.026.0808263
TATAATG74750.025.5618782
TAATGGA72600.025.3359014
ATGGGGA83900.024.9032365
GCAGAGT198250.022.604089
ATGGGGG72300.021.6534985
Produced by FastQC (version 0.11.5)