FastQCFastQC Report
Mon 15 Aug 2016
SRR2921087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5564621
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT114910.20650103573990036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108020.1941192401063792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92950.16703743165976623No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT75420.1355348369637393No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC65160.1170969235820373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63430.11398799666679905No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG62540.11238860651965336No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT57000.10243285212056671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99300.054.4256631
GTATCAA167950.046.812321
ATCAACG213550.036.4443553
TATCAAC215100.036.321242
TCAACGC214200.036.2503054
CAACGCA215950.035.901355
AACGCAG221850.034.9309776
TACATGG222950.031.6477342
CATGGGG153700.030.9396724
ACGCAGA252500.030.6055417
ACATGGG225600.030.5883563
CGCAGAG258550.029.8433598
GTACATG254150.028.2123411
GTATAAT64700.027.3827441
GCAGAGT290850.026.0175519
TATAATG74850.024.283232
GAGTACT163150.023.97459212-13
AGAGTAC264900.023.60246810-11
ATAATGG73350.023.3167723
CAGAGTA276650.022.8903110-11
Produced by FastQC (version 0.11.5)