FastQCFastQC Report
Mon 15 Aug 2016
SRR2921086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5825598
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84250.041.541781
GTATCAA124700.039.7808231
GTACATG223750.032.8828161
AACGCAG149600.032.222536
TCAACGC149350.032.0810934
TACATGG226300.031.6662522
CAACGCA155700.030.810675
ATCAACG157650.030.5430953
ACATGGG234700.029.8991763
CATGGGG169050.029.7156834
TATCAAC168250.029.2199672
ACGCAGA171150.027.9890297
CGCAGAG180700.026.2790668
ATGGGGA78550.022.6558195
GCAGAGT200450.022.3836279
CAGAGTA181050.021.0653510-11
AGAGTAC169350.020.83428810-11
ATGGGGC85850.020.5906895
GTATAGA16700.019.6364571
ATGGGGG76150.017.038895
Produced by FastQC (version 0.11.5)