##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921086_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5825598
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.942691033607193	31.0	31.0	34.0	25.0	34.0
2	30.05281208899069	31.0	31.0	34.0	25.0	34.0
3	30.135120892310113	31.0	31.0	34.0	25.0	34.0
4	33.49716938930561	37.0	35.0	37.0	27.0	37.0
5	33.60352224784477	37.0	35.0	37.0	28.0	37.0
6	33.534846036406904	37.0	35.0	37.0	28.0	37.0
7	33.481776978088774	37.0	35.0	37.0	27.0	37.0
8	33.439146504788006	37.0	35.0	37.0	27.0	37.0
9	34.864883227438625	39.0	35.0	39.0	27.0	39.0
10-11	34.831994414993964	39.0	35.0	39.0	27.0	39.0
12-13	34.753325323855165	39.0	35.0	39.0	27.0	39.0
14-15	35.957063721183644	40.0	36.0	41.0	27.0	41.0
16-17	35.91936956515022	40.0	36.0	41.0	27.0	41.0
18-19	35.94325131943536	40.0	36.0	41.0	27.0	41.0
20-21	35.987675857482785	40.0	36.0	41.0	27.0	41.0
22-23	35.910430311188655	40.0	36.0	41.0	27.0	41.0
24-25	35.896606751787544	40.0	36.0	41.0	27.0	41.0
26-27	35.83673418591533	40.0	36.0	41.0	27.0	41.0
28-29	35.708695828308095	40.0	36.0	41.0	26.5	41.0
30-31	35.602661992811726	40.0	35.5	41.0	26.0	41.0
32-33	35.483307293088195	40.0	35.0	41.0	25.0	41.0
34-35	35.364641964653245	40.0	35.0	41.0	25.0	41.0
36-37	35.22713204721644	40.0	35.0	41.0	24.5	41.0
38-39	35.076839321903094	40.0	35.0	41.0	24.0	41.0
40-41	34.93606338782731	40.0	35.0	41.0	24.0	41.0
42-43	34.727809316743105	39.0	34.5	41.0	23.0	41.0
44-45	34.517951461807016	39.0	34.0	41.0	22.0	41.0
46-47	34.32187485301938	39.0	34.0	41.0	21.0	41.0
48-49	34.06767125023045	39.0	33.0	41.0	19.5	41.0
50-51	33.874755175348525	38.0	33.0	40.0	18.0	41.0
52-53	33.67332898699841	38.0	33.0	40.0	16.5	41.0
54-55	33.42240410340707	38.0	33.0	40.0	14.5	41.0
56-57	33.156256834062354	37.5	32.5	40.0	12.5	41.0
58-59	32.854813359933175	37.0	32.0	40.0	8.5	41.0
60-61	32.534732571660456	36.5	32.0	40.0	7.5	41.0
62-63	32.23698871772477	36.0	31.5	39.5	7.0	41.0
64-65	31.901729745169508	35.5	31.0	39.0	4.0	41.0
66-67	31.540831945492975	35.0	31.0	39.0	2.0	40.5
68-69	31.170950947868356	35.0	31.0	38.0	2.0	40.0
70-71	30.810129878512043	35.0	30.0	37.0	2.0	40.0
72-73	30.45372681053516	35.0	30.0	37.0	2.0	39.0
74-75	30.408949347345974	35.0	30.0	36.5	2.0	39.0
76-77	30.479485796994574	35.0	31.0	36.0	2.0	39.0
78-79	30.320831784136153	35.0	31.0	36.0	2.0	37.5
80-81	30.116478170996352	35.0	31.0	35.0	2.0	37.0
82-83	29.885545226429976	35.0	31.0	35.0	2.0	37.0
84-85	29.645295040955453	35.0	30.5	35.0	2.0	36.0
86-87	29.50144603180652	35.0	30.5	35.0	2.0	36.0
88-89	29.33135070425388	35.0	30.0	35.0	2.0	36.0
90-91	29.1912692911526	35.0	30.0	35.0	2.0	35.0
92-93	29.080323685225103	35.0	30.0	35.0	2.0	35.0
94-95	28.96428667065596	35.0	30.0	35.0	2.0	35.0
96-97	28.85904683090045	34.5	30.0	35.0	2.0	35.0
98-99	28.7518368071398	34.0	30.0	35.0	2.0	35.0
100-101	28.616591464086603	34.0	29.0	35.0	2.0	35.0
102-103	27.289609925024006	32.5	26.0	34.5	2.0	35.0
104-105	28.253775921373222	34.0	29.0	35.0	2.0	35.0
106-107	28.392123006084525	34.0	29.0	35.0	2.0	35.0
108-109	28.320152025594624	34.0	29.0	35.0	2.0	35.0
110-111	28.212155129825298	34.0	29.0	35.0	2.0	35.0
112-113	28.092008837547667	34.0	29.0	35.0	2.0	35.0
114-115	27.939130884074046	34.0	29.0	35.0	2.0	35.0
116-117	27.782305095545553	34.0	29.0	35.0	2.0	35.0
118-119	27.560915720583534	34.0	27.5	35.0	2.0	35.0
120-121	27.338763076339973	34.0	27.0	35.0	2.0	35.0
122-123	27.017052154989067	34.0	27.0	35.0	2.0	35.0
124-125	25.663241782217035	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	213587.0
3	32460.0
4	21852.0
5	21333.0
6	28789.0
7	31177.0
8	29094.0
9	30965.0
10	32873.0
11	35050.0
12	35729.0
13	35398.0
14	35641.0
15	31393.0
16	25219.0
17	23114.0
18	22215.0
19	22147.0
20	22927.0
21	25283.0
22	29623.0
23	37958.0
24	50507.0
25	63070.0
26	70181.0
27	78175.0
28	90777.0
29	107290.0
30	128280.0
31	155587.0
32	195037.0
33	253901.0
34	349299.0
35	537103.0
36	1005337.0
37	1488636.0
38	428196.0
39	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4698857937333	17.295913984935794	11.615827518189912	25.618372703140995
2	16.763601427989116	20.06888812430507	37.47016505562977	25.697345392076045
3	19.172997236086076	23.890341556130437	28.656525501310494	28.280135706472993
4	13.239596467154847	16.02225577684849	35.010980655045934	35.72716710095073
5	14.428770363014246	37.37448015951082	33.403392783355486	14.793356694119456
6	33.646282303046846	35.604238543355635	16.481914143613707	14.267565009983821
7	29.233250747849166	32.28397010424949	20.131255755908082	18.351523391993258
8	26.915404520800447	34.468431144697306	19.399273903095658	19.216890431406593
9	26.731555660199824	15.770603343899921	18.461362634491994	39.036478361408264
10-11	25.547345610049373	25.765132632726374	26.901731746182843	21.785790011041414
12-13	26.035415784728215	23.481834587747606	26.65638349341089	23.82636613411329
14-15	23.60873173846709	24.817334198283795	23.9723322382277	27.601601825021415
16-17	23.861530335536475	27.313623971927914	24.85607076716781	23.9687749253678
18-19	23.89900658909278	26.48023929348665	26.185812914642465	23.434941202778102
20-21	25.053123971281444	25.650691333232995	25.687519028071858	23.6086656674137
22-23	24.696127500190677	25.45996156222021	25.514115611551674	24.32979532603744
24-25	24.188668703874836	25.68032496373725	25.75598582372497	24.375020508662946
26-27	24.02566491538026	25.81129835313786	25.783918663335054	24.37911806814682
28-29	24.119440752444167	25.93704169753515	25.773375745138814	24.17014180488187
30-31	24.29618582168363	25.91053411828212	25.524144356403905	24.26913570363035
32-33	23.968598254420048	26.033875893313375	25.514734408831607	24.48279144343497
34-35	24.30135581027985	25.93624665333973	25.60713638069585	24.15526115568457
36-37	24.44939472204426	25.78489589212289	25.459144833245112	24.306564552587737
38-39	24.224394576310786	25.93963714948816	25.490411655468954	24.345556618732104
40-41	24.265785429642996	25.9523547516779	25.67091624889431	24.110943569784798
42-43	24.21818551009659	25.852952565110837	25.547794101225996	24.38106782356658
44-45	24.082986035030824	25.946290350800417	25.59310126898123	24.37762234518753
46-47	24.13878424652387	25.95975463659219	25.513338756042252	24.388122360841685
48-49	24.133702503787124	25.94865315137277	25.407359464184093	24.510284880656013
50-51	23.90626395674012	26.19285895876707	25.395220525082625	24.505656559410195
52-53	24.125182081043523	25.94404264497379	25.381947146633934	24.548828127348756
54-55	23.968079669911255	25.949184248781894	25.439427249673642	24.643308831633213
56-57	23.92556713206968	26.01422789266951	25.337471738111105	24.7227332371497
58-59	23.90620881608203	25.698730458559698	25.53234796674205	24.86271275861622
60-61	23.83732271708098	25.738347586746453	25.621432405527923	24.802897290644648
62-63	23.79936076778649	25.7242302373313	25.61603648710742	24.860372507774787
64-65	23.822712730370558	25.69234748135807	25.570462955908468	24.914476832362908
66-67	23.79301252358748	25.775039230152807	25.508617750151807	24.923330496107905
68-69	24.036208586332748	25.512279258099348	25.520035664005896	24.93147649156201
70-71	23.807132650953285	25.36113989793074	25.721453054626338	25.110274396489636
72-73	23.83850464297563	25.416524642547945	25.68024507415699	25.06472564031944
74-75	23.65022079759965	25.620519169437483	25.710322620659536	25.018937412303334
76-77	23.653024735091005	25.34057150603492	25.898854749196058	25.107549009678014
78-79	23.635295461592438	25.43246671928937	25.852938726043856	25.07929909307433
80-81	23.655913147187768	25.34379581782378	25.908290305167537	25.092000729820917
82-83	23.630450688184027	25.225465218656357	25.996767035409956	25.147317057749664
84-85	23.554814524379058	25.219248132082544	25.965616161046906	25.26032118249149
86-87	23.499692869548454	25.176218834591335	26.04641462241594	25.27767367344427
88-89	23.589142462136277	25.102609265308985	25.923663739275632	25.384584533279103
90-91	23.543772053191894	25.08436435781552	25.946038766543438	25.42582482244915
92-93	23.63367162292262	25.01447542784315	25.969587430982997	25.382265518251234
94-95	23.456175213242016	24.910340936385275	26.152570398300316	25.48091345207239
96-97	23.51419071225375	24.850996254388257	26.110771397430906	25.524041635927087
98-99	23.4400321434596	24.980142240649243	25.93452573795797	25.645299877933187
100-101	23.42234985297065	25.146404397677045	25.966085189658884	25.465160559693416
102-103	23.33015658476255	25.118428138967246	25.978949982828038	25.572465293442164
104-105	23.11014864655139	25.28106181972965	25.88377977055733	25.725009763161633
106-107	23.107291514100645	25.38725820119774	25.884245823109175	25.621204461592452
108-109	23.159949372991214	25.416945615483005	25.859721468980478	25.563383542545303
110-111	23.238749466013093	25.611519702903557	25.730244546031884	25.41948628505147
112-113	23.294620511330834	25.709328990158625	25.689204415347174	25.306846083163364
114-115	23.20775718024615	25.845733472692174	25.635715906227773	25.3107934408339
116-117	23.098737334801054	25.81724489966839	25.71356539389408	25.370452371636482
118-119	23.192457011320496	25.846340164568048	25.589773761874497	25.371429062236956
120-121	23.253029760044704	26.001613741218698	25.52892726934829	25.216429229388304
122-123	23.348393807592956	26.13210616109608	25.346780755979857	25.172719275331108
124-125	23.41518411593104	26.198645717279156	25.175445779072568	25.21072438771724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2495.0
1	2021.0
2	1347.5
3	1006.0
4	771.5
5	653.0
6	611.0
7	669.0
8	748.5
9	803.0
10	836.5
11	859.5
12	962.5
13	1086.0
14	1269.0
15	1501.0
16	1738.5
17	2095.0
18	2489.5
19	2979.0
20	3685.0
21	4488.0
22	5487.5
23	6940.5
24	8950.5
25	11491.0
26	15449.0
27	20766.0
28	25468.5
29	31211.0
30	37833.5
31	45087.5
32	55074.0
33	63139.5
34	69393.0
35	77373.0
36	87837.5
37	98638.0
38	107362.0
39	116534.5
40	127154.0
41	137765.0
42	147965.5
43	162333.5
44	177341.0
45	190949.5
46	207426.5
47	220867.5
48	231485.5
49	242653.5
50	249343.0
51	251644.5
52	251364.0
53	252620.0
54	251517.5
55	243330.0
56	228935.5
57	209075.5
58	193949.5
59	179146.0
60	155644.5
61	129987.5
62	107593.0
63	84006.5
64	62562.5
65	47389.0
66	35563.5
67	27163.5
68	20972.5
69	16320.5
70	13371.5
71	10769.5
72	8697.0
73	7741.5
74	6490.0
75	4940.0
76	3603.5
77	2482.5
78	1654.0
79	1126.0
80	754.0
81	510.0
82	351.5
83	235.5
84	169.0
85	130.0
86	98.0
87	76.5
88	65.0
89	56.0
90	43.5
91	32.0
92	30.0
93	25.0
94	20.5
95	23.0
96	23.0
97	21.5
98	20.5
99	22.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2780315428562012
2	0.0684049946460432
3	0.07733111690851308
4	0.08423169604219172
5	0.09102928145745724
6	0.12331781217996848
7	0.11289828100050844
8	0.11226315307029423
9	0.11918089782370839
10-11	0.09770670753457412
12-13	0.08730434197484961
14-15	0.09632487514586485
16-17	0.09825600736611073
18-19	0.09093487054891189
20-21	0.09011950361147474
22-23	0.07281655891807158
24-25	0.08388838364748133
26-27	0.09596439713141895
28-29	0.08936421634311191
30-31	0.08447201471848899
32-33	0.09290033400862881
34-35	0.0867979561926518
36-37	0.08666921404463541
38-39	0.07669598897829888
40-41	0.08829136510964196
42-43	0.07765726368348794
44-45	0.07473052551858195
46-47	0.06496328788907164
48-49	0.06689442010931752
50-51	0.06931477249202571
52-53	0.0806955783766748
54-55	0.08551053471248789
56-57	0.09408476177037962
58-59	0.0647401348325099
60-61	0.08087581738389775
62-63	0.08619715950190864
64-65	0.07749419029600052
66-67	0.07180378735367597
68-69	0.07901334764259395
70-71	0.07811215260647919
72-73	0.06048306113810119
74-75	0.08769915122876655
76-77	0.09174165467648128
78-79	0.07352034932722787
80-81	0.08569077371971083
82-83	0.09000792708319386
84-85	0.09406759615064411
86-87	0.08207741076538408
88-89	0.06237127930900828
90-91	0.08603408611442122
92-93	0.06287766509120608
94-95	0.08953587254046709
96-97	0.07758001839467811
98-99	0.07202694041023772
100-101	0.08860034626488131
102-103	0.06192497319588478
104-105	0.06813892754014267
106-107	0.08635165007952832
108-109	0.09242827946590204
110-111	0.08576801900852067
112-113	0.07479060518765628
114-115	0.08810254329255125
116-117	0.08197441704697098
118-119	0.07377783362326065
120-121	0.10645259078982106
122-123	0.08502989735989336
124-125	0.10655558450823417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	5825598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0047310113628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.869954228358	31.89483226834846
2	13.491898361767909	12.143985132080227
3	5.6655390835677775	7.649281874709923
4	2.967972068856548	5.342911384325076
5	1.7961758563645027	4.041820563239434
6	1.1333016843695893	3.0602362475866656
7	0.7394823664548852	2.329614349296369
8	0.5381933885283513	1.937699894624986
9	0.39894913789034575	1.6159138774173094
>10	2.1503846118496943	17.773915403877545
>50	0.16819889220104053	5.202450549812951
>100	0.0770798277760097	5.893330274675196
>500	0.002258125366293663	0.6721966077106423
>1k	6.123666491164578E-4	0.44181157229521567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4331239471038E-5
2	0.0	0.0	0.0	0.0	3.4331239471038E-5
3	0.0	0.0	0.0	1.7165619735519E-5	3.4331239471038E-5
4	0.0	0.0	0.0	3.4331239471038E-5	3.4331239471038E-5
5	0.0	0.0	0.0	5.149685920655699E-5	6.8662478942076E-5
6	0.0	0.0	0.0	6.8662478942076E-5	8.582809867759499E-5
7	0.0	0.0	0.0	8.582809867759499E-5	8.582809867759499E-5
8	0.0	0.0	0.0	1.2015933814863298E-4	8.582809867759499E-5
9	0.0	0.0	0.0	1.37324957884152E-4	8.582809867759499E-5
10-11	0.0	0.0	0.0	1.5449057761967097E-4	8.582809867759499E-5
12-13	0.0	0.0	0.0	1.5449057761967097E-4	1.7165619735518998E-4
14-15	0.0	0.0	0.0	1.6307338748743048E-4	2.1457024669398748E-4
16-17	0.0	0.0	0.0	2.4031867629726596E-4	2.5748429603278497E-4
18-19	0.0	0.0	0.0	2.74649915768304E-4	2.74649915768304E-4
20-21	0.0	0.0	0.0	3.0039834537158247E-4	3.4331239471037996E-4
22-23	0.0	0.0	0.0	4.2914049338797495E-4	4.1197487365245594E-4
24-25	0.0	0.0	0.0	6.866247894207599E-4	4.1197487365245594E-4
26-27	0.0	0.0	0.0	0.0013818323887092791	4.3772330325573443E-4
28-29	0.0	0.0	0.0	0.005038109392374826	4.463061131234939E-4
30-31	0.0	0.0	0.0	0.018195556919650135	4.548889229912534E-4
32-33	0.0	0.0	0.0	0.05314475870116682	4.634717328590129E-4
34-35	0.0	0.0	0.0	0.10809190747456313	4.634717328590129E-4
36-37	0.0	0.0	0.0	0.20179902561076135	4.634717328590129E-4
38-39	0.0	0.0	0.0	0.35742253413297653	4.7205454272677245E-4
40-41	0.0	0.0	0.0	0.5362625433474812	4.806373525945319E-4
42-43	0.0	0.0	0.0	0.7128281079470297	4.978029723300509E-4
44-45	0.0	0.0	0.0	0.9296041367770314	5.149685920655699E-4
46-47	0.0	0.0	0.0	1.1951906053249812	5.321342118010889E-4
48-49	0.0	0.0	0.0	1.477410559396649	5.321342118010889E-4
50-51	0.0	0.0	0.0	1.773938057517872	5.321342118010889E-4
52-53	0.0	0.0	0.0	2.0753663400735856	5.321342118010889E-4
54-55	0.0	0.0	0.0	2.4161296402532413	5.664654512721269E-4
56-57	0.0	0.0	0.0	2.8219849704699844	5.83631071007646E-4
58-59	0.0	0.0	0.0	3.264866542456242	6.093795006109244E-4
60-61	0.0	0.0	0.0	3.735376179406818	6.265451203464434E-4
62-63	0.0	0.0	0.0	4.211825120785884	6.694591696852409E-4
64-65	0.0	0.0	0.0	4.719644232231609	6.694591696852409E-4
66-67	0.0	0.0	0.0	5.312647731614849	6.694591696852409E-4
68-69	0.0	0.0	0.0	5.97383307258757	6.694591696852409E-4
70-71	0.0	0.0	0.0	6.6869873273095735	6.780419795530004E-4
72-73	0.0	0.0	0.0	7.401918223674205	7.209560288917978E-4
74-75	0.0	0.0	0.0	8.134152064732238	7.552872683628359E-4
76-77	0.0	0.0	0.0	8.949973891092382	7.552872683628359E-4
78-79	0.0	0.0	0.0	9.849460948043445	7.552872683628359E-4
80-81	0.0	0.0	0.0	10.802710726006154	7.724528880983549E-4
82-83	0.0	0.0	0.0	11.773735503204993	7.896185078338739E-4
84-85	0.0	0.0	0.0	12.729946350572078	7.896185078338739E-4
86-87	0.0	0.0	0.0	13.746434271640439	8.840294163792283E-4
88-89	0.0	0.0	0.0	14.837781460375398	9.784403249245828E-4
90-91	0.0	0.0	0.0	15.995654008395363	9.784403249245828E-4
92-93	0.0	1.7165619735519E-5	0.0	17.162813156692238	0.001012771564395621
94-95	0.0	1.7165619735519E-5	0.0	18.316334220109248	0.0010642684236021778
96-97	0.0	1.7165619735519E-5	0.0	19.489475586883955	0.001184427761750811
98-99	0.0	1.7165619735519E-5	0.0	20.72139203563308	0.0012960042900316843
100-101	0.0	1.7165619735519E-5	0.0	22.02599630115226	0.0013045870998994438
102-103	0.0	1.7165619735519E-5	0.0	23.330600566671436	0.0013131699097672033
104-105	0.0	1.7165619735519E-5	0.0	24.606804314338202	0.0013217527196349628
106-107	0.0	1.7165619735519E-5	0.0	25.875223453454907	0.0013217527196349628
108-109	0.0	1.7165619735519E-5	0.0	27.168721563005207	0.0013217527196349628
110-111	0.0	1.7165619735519E-5	0.0	28.50739100088952	0.0013217527196349628
112-113	0.0	1.7165619735519E-5	0.0	29.868118260133983	0.0013389183393704817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8425	0.0	41.54178	1
GTATCAA	12470	0.0	39.780823	1
GTACATG	22375	0.0	32.882816	1
AACGCAG	14960	0.0	32.22253	6
TCAACGC	14935	0.0	32.081093	4
TACATGG	22630	0.0	31.666252	2
CAACGCA	15570	0.0	30.81067	5
ATCAACG	15765	0.0	30.543095	3
ACATGGG	23470	0.0	29.899176	3
CATGGGG	16905	0.0	29.715683	4
TATCAAC	16825	0.0	29.219967	2
ACGCAGA	17115	0.0	27.989029	7
CGCAGAG	18070	0.0	26.279066	8
ATGGGGA	7855	0.0	22.655819	5
GCAGAGT	20045	0.0	22.383627	9
CAGAGTA	18105	0.0	21.06535	10-11
AGAGTAC	16935	0.0	20.834288	10-11
ATGGGGC	8585	0.0	20.590689	5
GTATAGA	1670	0.0	19.636457	1
ATGGGGG	7615	0.0	17.03889	5
>>END_MODULE