FastQCFastQC Report
Mon 15 Aug 2016
SRR2921086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5825598
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119160.20454552476844434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102560.17605059600748282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67060.1151126459463904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102250.056.1088031
GTATCAA171850.050.6319851
TCAACGC226750.037.9173974
ATCAACG230600.037.4393733
CAACGCA233750.036.7309235
TATCAAC239850.036.2439082
AACGCAG239250.036.0031436
ACGCAGA273600.031.3317417
CGCAGAG285550.029.9791268
GTACATG211500.029.4380721
TACATGG218250.028.1737652
ACATGGG219000.027.3427013
GCAGAGT311350.026.9780949
CATGGGG158750.025.897324
AGAGTAC280350.025.05931510-11
GAGTACT186350.024.15025712-13
AGTACTT194650.023.89981712-13
CAGAGTA293550.023.4967910-11
GTACTTT198950.022.442714-15
TACTTTT224350.020.6841114-15
Produced by FastQC (version 0.11.5)