##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5825598
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240453941380782	31.0	31.0	34.0	28.0	34.0
2	31.354019793332803	33.0	31.0	34.0	28.0	34.0
3	31.443075028520678	34.0	31.0	34.0	28.0	34.0
4	34.929171734815895	37.0	35.0	37.0	32.0	37.0
5	34.68071243501525	37.0	35.0	37.0	32.0	37.0
6	34.44995415062969	37.0	35.0	37.0	30.0	37.0
7	34.388259540050655	37.0	35.0	37.0	30.0	37.0
8	34.37933444772537	37.0	35.0	37.0	30.0	37.0
9	35.94014777538718	39.0	37.0	39.0	30.0	39.0
10-11	35.898798801427766	39.0	37.0	39.0	30.0	39.0
12-13	35.8512678011768	39.0	37.0	39.0	30.0	39.0
14-15	37.09651970492986	40.0	37.0	41.0	31.0	41.0
16-17	37.078763158734944	40.0	37.0	41.0	31.0	41.0
18-19	37.096856751873375	40.0	37.0	41.0	31.0	41.0
20-21	37.09687039854106	40.0	37.0	41.0	30.0	41.0
22-23	37.052855260524325	40.0	37.0	41.0	30.0	41.0
24-25	37.00652714107633	40.0	37.0	41.0	30.0	41.0
26-27	36.88791837335841	40.0	37.0	41.0	30.0	41.0
28-29	36.75899117996127	40.0	37.0	41.0	30.0	41.0
30-31	36.619482326106265	40.0	36.5	41.0	30.0	41.0
32-33	36.49146945944433	40.0	36.0	41.0	30.0	41.0
34-35	36.341226943568714	40.0	36.0	41.0	29.5	41.0
36-37	36.15759935718187	40.0	35.5	41.0	28.5	41.0
38-39	36.00859740064453	40.0	35.0	41.0	28.0	41.0
40-41	35.86472187061311	40.0	35.0	41.0	27.0	41.0
42-43	35.693657629654496	40.0	35.0	41.0	27.0	41.0
44-45	35.503274513620745	40.0	35.0	41.0	26.0	41.0
46-47	35.21247578360196	39.5	35.0	41.0	24.5	41.0
48-49	34.93420254881988	39.0	35.0	41.0	23.5	41.0
50-51	34.718946878929856	39.0	35.0	41.0	23.0	41.0
52-53	34.49306920937559	39.0	34.0	41.0	22.0	41.0
54-55	34.26463678063608	38.5	34.0	41.0	22.0	41.0
56-57	34.00599852581658	38.0	34.0	40.5	20.5	41.0
58-59	33.737756793379845	37.5	34.0	40.0	20.0	41.0
60-61	33.44348666008193	37.0	33.0	40.0	18.5	41.0
62-63	33.14577662241713	36.5	33.0	40.0	18.0	41.0
64-65	32.82323076532229	36.0	33.0	39.5	16.0	41.0
66-67	32.48565572152421	35.5	32.5	39.0	13.5	41.0
68-69	32.13139869932667	35.0	32.0	39.0	12.0	40.5
70-71	31.76192607179555	35.0	32.0	37.5	8.0	40.0
72-73	31.397054602806442	35.0	32.0	37.0	7.0	39.5
74-75	31.089159516327765	35.0	31.0	36.5	7.0	39.0
76-77	30.811747652344017	35.0	31.0	36.0	4.0	39.0
78-79	30.535295861472076	35.0	31.0	35.5	2.0	37.5
80-81	30.515847643452226	35.0	31.0	35.0	2.0	37.0
82-83	30.338872850478182	35.0	31.0	35.0	2.0	37.0
84-85	30.161462222419054	35.0	31.0	35.0	2.0	36.0
86-87	30.010879569788372	35.0	31.0	35.0	2.0	36.0
88-89	29.850607697269876	35.0	31.0	35.0	2.0	36.0
90-91	29.712355624263807	35.0	31.0	35.0	2.0	35.0
92-93	29.617283839358638	35.0	31.0	35.0	2.0	35.0
94-95	29.518813261745834	35.0	31.0	35.0	2.0	35.0
96-97	29.458047139538294	35.0	31.0	35.0	2.0	35.0
98-99	29.368948904472983	35.0	31.0	35.0	2.0	35.0
100-101	29.275595483931433	35.0	31.0	35.0	2.0	35.0
102-103	27.933134074819442	32.5	28.0	34.5	2.0	35.0
104-105	28.962132042066756	34.0	29.5	35.0	2.0	35.0
106-107	29.132651274598764	34.0	30.0	35.0	2.0	35.0
108-109	29.12892410358559	35.0	30.0	35.0	2.0	35.0
110-111	29.02980792701453	34.5	30.0	35.0	2.0	35.0
112-113	28.904474098624725	34.0	30.0	35.0	2.0	35.0
114-115	28.801705936454937	34.0	30.0	35.0	2.0	35.0
116-117	28.678603209490255	34.0	30.0	35.0	2.0	35.0
118-119	28.512558796539	34.0	29.5	35.0	2.0	35.0
120-121	28.637873656918998	35.0	30.0	35.0	2.0	35.0
122-123	28.523290827825743	35.0	30.5	35.0	2.0	35.0
124-125	27.799462046643107	34.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107412.0
3	40363.0
4	18345.0
5	16172.0
6	18649.0
7	22265.0
8	24193.0
9	26306.0
10	29900.0
11	34065.0
12	36500.0
13	42871.0
14	54459.0
15	49637.0
16	28514.0
17	24226.0
18	22593.0
19	21923.0
20	21712.0
21	22519.0
22	23840.0
23	27363.0
24	35231.0
25	45799.0
26	57120.0
27	67974.0
28	81539.0
29	98561.0
30	118711.0
31	145860.0
32	183492.0
33	238414.0
34	329701.0
35	511511.0
36	998608.0
37	1652871.0
38	545573.0
39	806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33896408517276	17.620328477908846	12.404165859989224	25.636541576929172
2	16.965672768450116	20.211182698304814	36.82141643757868	26.001728095666387
3	19.211263805020533	23.546509731704795	29.465987869399846	27.776238593874826
4	13.49679265778874	15.94969533338918	35.030995381592014	35.52251662723007
5	14.27477223668202	36.86635627851876	33.7720032549158	15.086868229883413
6	33.82815574692079	35.2942247603721	16.281005350603046	14.596614142104059
7	29.130181981236227	31.78739609085483	20.424276685228097	18.658145242680845
8	27.388799874194852	33.508272529516056	19.465427705733386	19.637499890555706
9	27.098267781421143	14.432934470978044	18.797627427080293	39.671170320520524
10-11	25.92182663614926	24.842072755282167	27.16075662252423	22.075343986044345
12-13	26.650156354975017	22.56015467000993	27.170967648069418	23.61872132694563
14-15	23.94635314815511	23.843906497237846	24.792286947446883	27.41745340716016
16-17	23.693935330310953	26.549035678496434	25.623419572518667	24.13360941867394
18-19	23.657967313493224	25.34984252332834	27.68099000886512	23.311200154313315
20-21	24.667461613346862	24.59309203353542	27.368812335924208	23.370634017193513
22-23	24.27551843424295	24.471306744892708	27.23942001812282	24.013754802741516
24-25	23.849512401872133	24.72065933616876	27.476541256328595	23.95328700563051
26-27	23.65983745917872	24.898367990159883	27.439874326992424	24.00192022366897
28-29	23.72281396592202	24.939727759362928	27.476793341672956	23.8606649330421
30-31	23.952500633562973	24.841076995242613	27.306074787215557	23.900347583978856
32-33	23.633713585733982	25.00335021935459	27.21966304476691	24.143273150144513
34-35	23.94839159410361	24.94118121747969	27.314965287346254	23.79546190107044
36-37	24.20039642903102	24.703680502478818	27.087045008662496	24.008878059827662
38-39	24.00159772863628	24.837752682904174	27.091638562054527	24.069011026405015
40-41	23.991344570568106	24.852646507866467	27.177559069313833	23.978449852251597
42-43	24.076827852865225	24.746837399044008	27.011788500631834	24.164546247458933
44-45	23.940636639680978	24.847179552692833	26.95943286786629	24.252750939759895
46-47	24.14074632353667	24.90069082664054	26.61228295930906	24.346279890513735
48-49	24.199273837050793	25.073618940944836	26.28412516044053	24.44298206156384
50-51	23.99568191960923	25.37658521251464	26.15263621022521	24.475096657650923
52-53	24.131700782299802	25.246967723416063	26.113090881684343	24.50824061259979
54-55	23.942250161169728	25.259189249743113	26.119470762395046	24.67908982669211
56-57	23.853032399085702	25.32438057036867	25.980824386235806	24.841762644309824
58-59	23.71710744019443	25.027869794859253	26.26293927170198	24.992083493244337
60-61	23.70435037894883	25.137754703489385	26.322157583311657	24.835737334250123
62-63	23.55451498126537	25.11721912005185	26.316475743698252	25.011790154984524
64-65	23.495813610727268	25.055725233305953	26.323351954770104	25.125109201196683
66-67	23.471126284246537	25.162428788652303	26.244251521016785	25.122193406084374
68-69	23.667857370955147	24.922947022300725	26.228323699421964	25.18087190732216
70-71	23.384499210744757	24.815872636773552	26.340967708398882	25.45866044408281
72-73	23.430602150545017	24.83472205385257	26.268599027512153	25.466076768090257
74-75	23.23862088288753	25.06614523056448	26.208190213956705	25.48704367259128
76-77	23.21410331570576	24.84496979050343	26.309248423432507	25.6316784703583
78-79	23.12805689910781	24.92311157574981	26.201928586321692	25.746902938820686
80-81	23.03406111722827	24.937664080578344	26.199911188429464	25.828363613763923
82-83	22.944006441497528	24.846462906236468	26.265583870160565	25.943946782105442
84-85	22.781939281192994	24.809848136027597	26.197891504936294	26.21032107784312
86-87	22.691026195987465	24.81687134655772	26.267162557575475	26.22493989987934
88-89	22.65899323065935	24.79387441415866	26.130470445782393	26.416661909399604
90-91	22.49068281659431	24.756602935401002	26.17418830088826	26.578525947116425
92-93	22.50917270103909	24.685761542134852	26.167162524304665	26.637903232521392
94-95	22.262648507370507	24.574775079611513	26.334103890545684	26.828472522472296
96-97	22.28639991717678	24.444988701515086	26.354807365188854	26.91380401611928
98-99	22.217864086604596	24.546705249473074	26.132580151350638	27.102850512571692
100-101	22.12593531677681	24.60253678316705	26.151919388457767	27.11960851159837
102-103	22.00461877824714	24.46518009148965	26.189272732142456	27.340928398120763
104-105	21.706591773667398	24.666834396494945	26.10216051298202	27.52441331685564
106-107	21.69826813096404	24.718718396139234	26.110243279926305	27.47277019297042
108-109	21.642329698374496	24.767562260576277	26.078768876754715	27.51133916429451
110-111	21.619117409461595	24.918706145573303	25.92978209521206	27.53239434975304
112-113	21.48897365898648	25.046487827666002	25.914309254661617	27.5502292586859
114-115	21.270386686077654	25.172140885305794	25.91622488360275	27.641247545013805
116-117	21.13196747913884	25.20065452366133	25.99435416260551	27.673023834594318
118-119	21.103848867198387	25.249556601798588	25.893613786969556	27.75298074403347
120-121	21.04348063428456	25.38113275693781	25.895097639680586	27.680288969097045
122-123	20.957229770216138	25.501621939620566	25.702530788871297	27.838617501291996
124-125	20.7595083378465	25.586524522598314	25.745393778607838	27.908573360947347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	124.0
2	264.0
3	426.0
4	328.0
5	285.0
6	530.5
7	1082.5
8	1746.5
9	1965.0
10	1699.5
11	1470.5
12	1597.5
13	1827.0
14	2057.0
15	2403.0
16	2800.5
17	3175.5
18	3623.0
19	4151.5
20	4732.5
21	5522.5
22	6578.5
23	7938.0
24	9629.5
25	11872.5
26	15608.5
27	20684.0
28	25394.0
29	31063.0
30	37637.5
31	45021.0
32	55080.5
33	63368.0
34	69273.5
35	76686.5
36	87267.0
37	98114.5
38	106908.5
39	115694.0
40	125729.5
41	136216.0
42	146289.5
43	160779.5
44	176435.0
45	189925.0
46	206051.0
47	220614.5
48	232298.5
49	243812.0
50	251857.0
51	254090.5
52	252016.5
53	253336.5
54	253037.5
55	243958.0
56	228796.0
57	209631.0
58	194856.0
59	179690.0
60	155942.5
61	130565.5
62	107956.5
63	83583.0
64	61430.5
65	46162.5
66	34498.0
67	26191.0
68	20080.0
69	15576.5
70	12819.0
71	10258.0
72	8313.5
73	7417.0
74	5971.5
75	4427.0
76	3213.0
77	2156.0
78	1369.0
79	840.0
80	529.5
81	340.5
82	218.5
83	144.0
84	109.0
85	69.0
86	52.5
87	47.0
88	33.0
89	23.5
90	17.0
91	16.0
92	17.5
93	17.0
94	13.0
95	19.5
96	26.5
97	16.0
98	7.5
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1641033246715616
2	0.01182711199777259
3	0.0
4	0.0036734426234010654
5	0.13622635822107876
6	0.0752712425402508
7	0.007501375824421802
8	0.012307749350367122
9	0.01198160257539226
10-11	0.00496086410356499
12-13	0.009466839284138726
14-15	0.010556856137344183
16-17	0.014230298760745249
18-19	0.018616114603170354
20-21	0.022452630614058847
22-23	0.024898731426370304
24-25	0.024666995559940803
26-27	0.02191191359239
28-29	0.020461418724738646
30-31	0.023928873911313483
32-33	0.023216500692289442
34-35	0.023225083502157202
36-37	0.023216500692289442
38-39	0.018530286504492757
40-41	0.025885754561162648
42-43	0.02193766202199328
44-45	0.030383146931868624
46-47	0.025551024976320027
48-49	0.017337275932874187
50-51	0.01351792554172121
52-53	0.01457361115545563
54-55	0.017045460397370364
56-57	0.013801158267357276
58-59	0.012762638273358374
60-61	0.01601552321323922
62-63	0.014127305042332134
64-65	0.025508110926981232
66-67	0.019268408153120076
68-69	0.017749250806526645
70-71	0.02465841275007304
72-73	0.030417478171339662
74-75	0.015706542057999883
76-77	0.007604369542834916
78-79	0.013071619428597716
80-81	0.015560634290247972
82-83	0.014856843881091692
84-85	0.013389183393704818
86-87	0.009037698790750753
88-89	0.020178185999102583
90-91	0.017345858742741947
92-93	0.014444869007439236
94-95	0.010170629693295007
96-97	0.019646051787301493
98-99	0.021319699711514595
100-101	0.026924274555161543
102-103	0.019122500385368165
104-105	0.012951460090449084
106-107	0.018066814771633743
108-109	0.009329514326254576
110-111	0.02329374598109928
112-113	0.016393166847420645
114-115	0.01232491497010264
116-117	0.01027362341170812
118-119	0.007149480619843662
120-121	0.0034331239471037996
122-123	0.00548441550549832
124-125	9.097778459825069E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	5825598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.58772632875728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.78556059387772	29.29420629942215
2	14.369564545382541	12.239341646443304
3	6.137532764973302	7.841506971853917
4	3.1860909934481727	5.427534851499567
5	1.893577884359933	4.032158836065401
6	1.1744964001385911	3.0011478757927748
7	0.8041497506165779	2.3972836654618064
8	0.5968054965830032	2.0333271327980023
9	0.45875277946647913	1.7583514042027637
>10	2.313472559150738	18.211608309703696
>50	0.18942272930188428	5.536332542712796
>100	0.08665150279303974	6.366777029318819
>500	0.0029516130969532966	0.8466310649450226
>1k	8.490884597598566E-4	0.5165304410859619
>5k	4.0432783788187885E-5	0.11547331857547855
>10k+	8.086556757637577E-5	0.3817886101186267
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11916	0.20454552476844434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10256	0.17605059600748282	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6706	0.1151126459463904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7165619735519E-5
2	0.0	0.0	0.0	0.0	1.7165619735519E-5
3	0.0	0.0	0.0	0.0	1.7165619735519E-5
4	0.0	0.0	0.0	3.4331239471038E-5	1.7165619735519E-5
5	0.0	0.0	0.0	5.149685920655699E-5	1.7165619735519E-5
6	0.0	0.0	0.0	5.149685920655699E-5	5.149685920655699E-5
7	0.0	0.0	0.0	5.149685920655699E-5	5.149685920655699E-5
8	0.0	0.0	0.0	8.582809867759499E-5	5.149685920655699E-5
9	0.0	0.0	0.0	1.2015933814863298E-4	5.149685920655699E-5
10-11	0.0	0.0	0.0	1.37324957884152E-4	5.149685920655699E-5
12-13	0.0	0.0	0.0	1.37324957884152E-4	1.1157652828087348E-4
14-15	0.0	0.0	0.0	1.459077677519115E-4	1.2015933814863298E-4
16-17	0.0	0.0	0.0	1.5449057761967097E-4	1.2015933814863298E-4
18-19	0.0	0.0	0.0	1.7165619735518998E-4	1.37324957884152E-4
20-21	0.0	0.0	0.0	2.1457024669398748E-4	1.5449057761967097E-4
22-23	0.0	0.0	0.0	3.3472958484262043E-4	2.489014861650255E-4
24-25	0.0	0.0	0.0	5.83631071007646E-4	2.5748429603278497E-4
26-27	0.0	0.0	0.0	0.0012788386702961654	2.5748429603278497E-4
28-29	0.0	0.0	0.0	0.004866453195019636	2.5748429603278497E-4
30-31	0.0	0.0	0.0	0.01814406006044358	2.5748429603278497E-4
32-33	0.0	0.0	0.0	0.05312759308143129	2.5748429603278497E-4
34-35	0.0	0.0	0.0	0.10786875441800138	3.4331239471037996E-4
36-37	0.0	0.0	0.0	0.2007690884266302	3.4331239471037996E-4
38-39	0.0	0.0	0.0	0.354658869355558	3.604780144458989E-4
40-41	0.0	0.0	0.0	0.5325461866747414	3.604780144458989E-4
42-43	0.0	0.0	0.0	0.7079187407026712	3.8622644404917746E-4
44-45	0.0	0.0	0.0	0.923973813503781	4.3772330325573443E-4
46-47	0.0	0.0	0.0	1.1888479088327069	4.7205454272677245E-4
48-49	0.0	0.0	0.0	1.470132336628789	5.321342118010889E-4
50-51	0.0	0.0	0.0	1.766728497228954	5.49299831536608E-4
52-53	0.0	0.0	0.0	2.067882129868899	6.694591696852409E-4
54-55	0.0	0.0	0.0	2.408130461456489	6.866247894207599E-4
56-57	0.0	0.0	0.0	2.814406349356753	7.123732190240384E-4
58-59	0.0	0.0	0.0	3.2568158668002836	7.381216486273169E-4
60-61	0.0	0.0	0.0	3.7275486568074214	7.381216486273169E-4
62-63	0.0	0.0	0.0	4.203971849756883	7.896185078338739E-4
64-65	0.0	0.0	0.0	4.711619305005255	8.239497473049119E-4
66-67	0.0	0.0	0.0	5.304356737282593	8.239497473049119E-4
68-69	0.0	0.0	0.0	5.965276011149413	8.239497473049119E-4
70-71	0.0	0.0	0.0	6.676936856954428	8.325325571726714E-4
72-73	0.0	0.0	0.0	7.388563371519971	8.754466065114689E-4
74-75	0.0	0.0	0.0	8.117098021525	9.183606558502664E-4
76-77	0.0	0.0	0.0	8.929263570881478	9.355262755857854E-4
78-79	0.0	0.0	0.0	9.82779793593722	9.612747051890639E-4
80-81	0.0	0.0	0.0	10.778618778707354	9.784403249245828E-4
82-83	0.0	0.0	0.0	11.750175690117992	9.784403249245828E-4
84-85	0.0	0.0	0.0	12.70588873451275	9.784403249245828E-4
86-87	0.0	0.0	0.0	13.720273867163508	0.001012771564395621
88-89	0.0	0.0	0.0	14.809672758058486	0.0010814340433376968
90-91	0.0	0.0	0.0	15.967133331204796	0.001098599663073216
92-93	0.0	0.0	0.0	17.133648768761592	0.0012015933814863299
94-95	0.0	0.0	0.0	18.287753463249608	0.0012015933814863299
96-97	0.0	0.0	0.0	19.460517186390135	0.0012702558604284057
98-99	0.0	0.0	0.0	20.69285419282278	0.0013217527196349628
100-101	0.0	0.0	0.0	22.000033301302288	0.0013217527196349628
102-103	0.0	0.0	0.0	23.30641420846409	0.0013303355295027222
104-105	0.0	0.0	0.0	24.584523339921496	0.0013389183393704817
106-107	0.0	0.0	0.0	25.854350059856515	0.0013389183393704817
108-109	0.0	0.0	0.0	27.15004536873296	0.0013389183393704817
110-111	0.0	0.0	0.0	28.492474077339356	0.0013389183393704817
112-113	0.0	0.0	0.0	29.85823086316632	0.0013389183393704817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10225	0.0	56.108803	1
GTATCAA	17185	0.0	50.631985	1
TCAACGC	22675	0.0	37.917397	4
ATCAACG	23060	0.0	37.439373	3
CAACGCA	23375	0.0	36.730923	5
TATCAAC	23985	0.0	36.243908	2
AACGCAG	23925	0.0	36.003143	6
ACGCAGA	27360	0.0	31.331741	7
CGCAGAG	28555	0.0	29.979126	8
GTACATG	21150	0.0	29.438072	1
TACATGG	21825	0.0	28.173765	2
ACATGGG	21900	0.0	27.342701	3
GCAGAGT	31135	0.0	26.978094	9
CATGGGG	15875	0.0	25.89732	4
AGAGTAC	28035	0.0	25.059315	10-11
GAGTACT	18635	0.0	24.150257	12-13
AGTACTT	19465	0.0	23.899817	12-13
CAGAGTA	29355	0.0	23.49679	10-11
GTACTTT	19895	0.0	22.4427	14-15
TACTTTT	22435	0.0	20.68411	14-15
>>END_MODULE