FastQCFastQC Report
Mon 15 Aug 2016
SRR2921085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514106
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105850.034.9462781
GGTATCA76700.032.4370081
GTACATG167150.031.4819981
TACATGG171500.030.19552
TCAACGC119650.029.9986254
ATCAACG122300.029.5432993
AACGCAG122750.029.4789056
CAACGCA121800.029.4687655
TATCAAC125400.029.0496562
CATGGGG113950.028.8873394
ACATGGG175400.028.4727293
ACGCAGA137450.025.7610157
CGCAGAG138900.025.1067758
TATACCG4350.021.8938755
GCAGAGT162450.020.7710729
ATGGGGA65650.020.6724535
AGAGTAC135400.019.62231310-11
ATGGGGG59000.019.2696255
CAGAGTA151200.018.33924910-11
GTATAGA15100.018.1752741
Produced by FastQC (version 0.11.5)