##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921085_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4514106
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02555589080097	31.0	31.0	34.0	26.0	34.0
2	30.134614251415453	31.0	31.0	34.0	26.0	34.0
3	30.218841781739286	31.0	31.0	34.0	26.0	34.0
4	33.59200071952232	37.0	35.0	37.0	28.0	37.0
5	33.68036129413	37.0	35.0	37.0	28.0	37.0
6	33.63316922553436	37.0	35.0	37.0	28.0	37.0
7	33.577395834302514	37.0	35.0	37.0	28.0	37.0
8	33.54176175747756	37.0	35.0	37.0	28.0	37.0
9	34.98962120074274	39.0	35.0	39.0	27.0	39.0
10-11	34.95157036631395	39.0	35.0	39.0	27.0	39.0
12-13	34.881610555888585	39.0	35.0	39.0	27.0	39.0
14-15	36.09996087818939	40.0	36.0	41.0	27.0	41.0
16-17	36.07022287469545	40.0	36.0	41.0	27.0	41.0
18-19	36.09910279023133	40.0	36.0	41.0	27.0	41.0
20-21	36.146885119667104	40.0	36.0	41.0	27.0	41.0
22-23	36.069426149939765	40.0	36.0	41.0	27.0	41.0
24-25	36.05091661560451	40.0	36.0	41.0	27.0	41.0
26-27	35.9952967431425	40.0	36.0	41.0	27.0	41.0
28-29	35.8725235960343	40.0	36.0	41.0	27.0	41.0
30-31	35.77606241413029	40.0	36.0	41.0	27.0	41.0
32-33	35.670192392469296	40.0	36.0	41.0	26.0	41.0
34-35	35.5646758184234	40.0	35.0	41.0	25.5	41.0
36-37	35.44526280508256	40.0	35.0	41.0	25.0	41.0
38-39	35.31071124603631	40.0	35.0	41.0	25.0	41.0
40-41	35.191011464950094	40.0	35.0	41.0	24.0	41.0
42-43	35.003158986519146	40.0	35.0	41.0	24.0	41.0
44-45	34.818328811950806	39.0	35.0	41.0	23.0	41.0
46-47	34.64643320294206	39.0	34.0	41.0	22.5	41.0
48-49	34.42949368047627	39.0	34.0	41.0	21.5	41.0
50-51	34.264408611583335	39.0	34.0	41.0	20.5	41.0
52-53	34.09189338930012	39.0	33.5	40.5	19.5	41.0
54-55	33.87145250909039	38.0	33.0	40.0	18.5	41.0
56-57	33.632916462307264	38.0	33.0	40.0	17.5	41.0
58-59	33.36365495183321	38.0	33.0	40.0	14.5	41.0
60-61	33.07192664505441	37.0	32.5	40.0	13.0	41.0
62-63	32.78761387083068	37.0	32.0	40.0	10.0	41.0
64-65	32.47571434964088	36.0	32.0	39.5	8.0	41.0
66-67	32.134515228485995	36.0	31.5	39.0	7.5	41.0
68-69	31.776034058571064	35.0	31.0	39.0	7.0	40.5
70-71	31.413806631922245	35.0	31.0	38.5	4.0	40.0
72-73	31.050385613452587	35.0	31.0	37.5	2.0	40.0
74-75	30.992046154875407	35.0	31.0	37.0	2.0	39.0
76-77	31.016978998720898	35.0	31.0	37.0	2.0	39.0
78-79	30.816716864867594	35.0	31.0	36.0	2.0	39.0
80-81	30.579190541826	35.0	31.0	36.0	2.0	37.5
82-83	30.311791858675893	35.0	31.0	35.5	2.0	37.0
84-85	30.036952278036892	35.0	31.0	35.0	2.0	37.0
86-87	29.871570694175105	35.0	31.0	35.0	2.0	36.0
88-89	29.679715983629983	35.0	31.0	35.0	2.0	36.0
90-91	29.522379182057314	35.0	31.0	35.0	2.0	36.0
92-93	29.398328373325747	35.0	31.0	35.0	2.0	35.5
94-95	29.275161239013883	35.0	30.0	35.0	2.0	35.0
96-97	29.16760040637061	35.0	30.0	35.0	2.0	35.0
98-99	29.055542670021484	35.0	30.0	35.0	2.0	35.0
100-101	28.91321792177676	35.0	30.0	35.0	2.0	35.0
102-103	27.617444295725445	33.0	27.5	34.5	2.0	35.0
104-105	28.55845044400818	34.0	29.0	35.0	2.0	35.0
106-107	28.684280896372393	34.0	30.0	35.0	2.0	35.0
108-109	28.608987582480342	34.0	29.5	35.0	2.0	35.0
110-111	28.49608405296641	34.0	29.0	35.0	2.0	35.0
112-113	28.373644415970737	34.0	29.0	35.0	2.0	35.0
114-115	28.219715487407697	34.0	29.0	35.0	2.0	35.0
116-117	28.06988305104045	34.0	29.0	35.0	2.0	35.0
118-119	27.856629197453493	34.0	29.0	35.0	2.0	35.0
120-121	27.6397349774241	34.0	29.0	35.0	2.0	35.0
122-123	27.3341017025298	34.0	28.0	35.0	2.0	35.0
124-125	26.086986105332926	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162381.0
3	23443.0
4	15282.0
5	15003.0
6	19856.0
7	20922.0
8	20087.0
9	21116.0
10	22881.0
11	24826.0
12	25616.0
13	24996.0
14	25227.0
15	22762.0
16	18777.0
17	17736.0
18	16782.0
19	16568.0
20	17014.0
21	18232.0
22	22251.0
23	28343.0
24	38351.0
25	48698.0
26	54269.0
27	59441.0
28	69783.0
29	82155.0
30	96779.0
31	116749.0
32	146638.0
33	188887.0
34	257815.0
35	384167.0
36	710950.0
37	1215354.0
38	443398.0
39	571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.731920940544555	18.014328920874114	12.015218063140654	25.238532075440677
2	16.08804260692522	20.417724863971394	38.8625472641757	24.63168526492768
3	18.889128584449413	24.704795702152673	29.212316292013636	27.19375942138428
4	12.756503438079536	16.610207898935627	36.586279255604566	34.04700940738027
5	13.722682768094426	37.992813340887345	34.4037412314887	13.88076265952953
6	32.65266874596988	36.66139410027104	17.145511963535224	13.540425190223859
7	28.123620034870218	32.68540354810313	21.127131675316203	18.06384474171045
8	25.964893914788494	35.529463481323134	19.961989330042535	18.543653273845827
9	26.382796275146564	15.990217413243975	19.15354362582537	38.473442685784086
10-11	24.8876631609013	26.37163153028909	27.915496618307994	20.82520869050161
12-13	25.51260794875334	24.085275842705663	27.459380115422498	22.942736093118498
14-15	23.00410266030944	25.49137789379918	24.851053761237633	26.653465684653753
16-17	23.034559639794193	28.0186906437233	25.73493897887579	23.21181073760673
18-19	23.081137870873725	27.37675829911862	27.054900531203906	22.48720329880375
20-21	24.237397464669616	26.5868986717111	26.48937743622925	22.68632642739003
22-23	23.714674606154347	26.487276634013718	26.332774540786367	23.465274219045572
24-25	23.35215125645723	26.524779481967208	26.677138806517515	23.445930455058043
26-27	23.320135226084577	26.649550891066276	26.599578444101713	23.43073543874744
28-29	23.338257016248154	26.879106787819644	26.556811218502315	23.225824977429887
30-31	23.63961072724011	26.81781203804569	26.362950219409935	23.17962701530426
32-33	23.256095521975013	26.869491500618015	26.371145963009763	23.503267014397217
34-35	23.440744937260387	26.83269732655419	26.50510764072897	23.22145009545645
36-37	23.59098711585113	26.67948876715482	26.34922802980052	23.380296087193535
38-39	23.415918349428935	26.86171026498656	26.421610600703893	23.300760784880616
40-41	23.53657911933207	26.756558511002748	26.467467599089723	23.239394770575455
42-43	23.375708015331067	26.71452950445412	26.457674058087687	23.452088422127126
44-45	23.48784587736222	26.81525893218602	26.335066775509336	23.361828414942426
46-47	23.3583600315324	26.733095894492383	26.315209593794254	23.593334480180967
48-49	23.16506836226302	26.75584405680384	26.463757603381378	23.615329977551763
50-51	23.17534810592423	26.922696630409142	26.355378416102525	23.5465768475641
52-53	23.324451559225285	26.795593738376972	26.197556745335127	23.68239795706262
54-55	23.29064502971714	26.703650902634056	26.250762343929445	23.75494172371935
56-57	23.165650084243364	26.79244998507613	26.253239233857716	23.788660696822788
58-59	23.15490527973047	26.543843794880466	26.402576136838867	23.898674788550192
60-61	23.29771995362903	26.483656473028223	26.38240705768179	23.836216515660954
62-63	23.087229467273982	26.503426463303605	26.53052241979752	23.878821649624893
64-65	22.96921326230336	26.48404403030617	26.43157526573883	24.115167441651643
66-67	23.0450510808894	26.559080617151942	26.351790707692185	24.044077594266472
68-69	23.135709218651282	26.33747064029353	26.372513272909824	24.15430686814536
70-71	22.96956499519376	26.105664976492054	26.574604456682156	24.350165571632026
72-73	23.001935879022746	26.07042494240355	26.59669009686932	24.330949081704382
74-75	22.854189621189125	26.24714983270962	26.573382148257256	24.325278397843995
76-77	22.752330989925653	26.124072881575422	26.782014301705313	24.34158182679361
78-79	22.852388223267244	26.10448716513317	26.708080524342492	24.335044087257096
80-81	22.76329329791218	26.057584575169024	26.72367133540861	24.455450791510188
82-83	22.733847391510277	26.009937167053682	26.85606208434374	24.4001533570923
84-85	22.772890007994135	25.958304089871397	26.708111997747007	24.560693904387463
86-87	22.59155865817181	25.874113004798893	26.905220848182875	24.629107488846426
88-89	22.73097627449509	25.74753689926572	26.82935155739879	24.6921352688404
90-91	22.595156898570263	25.805479919821195	26.826595593713414	24.772767587895128
92-93	22.56519089580829	25.724353754948158	26.957156705765723	24.753298643477827
94-95	22.42794993573388	25.662764799085362	27.030153576789555	24.879131688391197
96-97	22.462004030347554	25.582419365888732	26.972543395854938	24.983033207908775
98-99	22.39920914572685	25.62192035566272	26.885021401418513	25.093849097191917
100-101	22.332400258812875	25.66747645472482	26.942171997032254	25.057951289430047
102-103	22.16171628867515	25.63385661298203	26.963894768297124	25.24053233004569
104-105	21.972934015022766	25.706600271262854	26.956391326150797	25.364074387563583
106-107	21.921899482355048	25.723542717654286	26.94769303702074	25.40686476296992
108-109	21.906194286018398	25.72073380580268	26.97635509708092	25.396716811098003
110-111	21.941056957889625	25.83709332700071	26.822540937533883	25.39930877757578
112-113	21.87234402001482	25.927763219637207	26.783696158605608	25.416196601742374
114-115	21.81917217609108	25.905407454974096	26.807832547793186	25.467587821141645
116-117	21.6841306594456	25.900754234294876	26.912364302902553	25.50275080335697
118-119	21.774032275085823	25.824558206273572	26.751932611990398	25.64947690665021
120-121	21.790787776339045	25.870848960955996	26.758877541277936	25.579485721427027
122-123	21.743992147143935	25.921553701852446	26.689533135777626	25.644921015225997
124-125	21.788947995708114	26.030467241549854	26.46259437291056	25.71799038983148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2660.0
1	1953.0
2	1065.0
3	821.5
4	641.0
5	473.5
6	452.0
7	500.5
8	564.5
9	641.0
10	637.5
11	618.5
12	687.0
13	726.5
14	817.5
15	1015.0
16	1205.5
17	1416.5
18	1678.0
19	2006.0
20	2411.0
21	3012.0
22	4091.0
23	5500.0
24	7123.5
25	9319.0
26	12898.0
27	17312.0
28	21776.5
29	28089.0
30	35006.5
31	42847.0
32	52419.5
33	61082.0
34	69767.5
35	78730.0
36	89460.0
37	99305.5
38	107264.5
39	115829.0
40	125346.5
41	134166.5
42	141618.5
43	151136.5
44	160717.0
45	169056.5
46	177512.5
47	182978.0
48	186716.5
49	192431.0
50	194998.0
51	190814.0
52	183799.5
53	179272.0
54	172934.5
55	162105.0
56	149748.0
57	135632.5
58	122678.5
59	109162.5
60	92140.5
61	75020.0
62	59889.5
63	45451.5
64	33520.5
65	25442.5
66	18899.0
67	13992.5
68	10724.5
69	8073.5
70	6204.0
71	4782.0
72	3605.0
73	2893.5
74	2228.0
75	1635.0
76	1127.5
77	773.0
78	535.5
79	405.5
80	324.0
81	244.0
82	176.5
83	131.5
84	98.5
85	74.0
86	55.0
87	42.5
88	33.0
89	28.5
90	25.5
91	19.5
92	22.5
93	19.0
94	15.0
95	16.0
96	16.5
97	17.5
98	16.0
99	18.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2910653848181678
2	0.07288264830289763
3	0.08318369130011569
4	0.0892757059758898
5	0.09660827636745792
6	0.12992605844878255
7	0.11933702930325518
8	0.11900473759366749
9	0.12598286349500876
10-11	0.10360855504943836
12-13	0.09201157438482835
14-15	0.10174772147574737
16-17	0.10376362451391262
18-19	0.09636459578042696
20-21	0.09567785958061242
22-23	0.0772578224791354
24-25	0.0895858449048383
26-27	0.10236799933364435
28-29	0.09460344971961226
30-31	0.08982952549186926
32-33	0.09933306838607689
34-35	0.09295306756199345
36-37	0.0926983105846429
38-39	0.0833830663258683
40-41	0.09479174835504528
42-43	0.0838925802805694
44-45	0.08019306591382658
46-47	0.07066737023897977
48-49	0.07274973161906256
50-51	0.07554098197959906
52-53	0.08713796264420906
54-55	0.09229956053313769
56-57	0.10083945746954104
58-59	0.07012462711331989
60-61	0.08764747659891017
62-63	0.09321890092966359
64-65	0.08425810116111584
66-67	0.07786702394671281
68-69	0.0860746291735285
70-71	0.08467900399326024
72-73	0.06594882796283473
74-75	0.09488035947760198
76-77	0.09822542935411796
78-79	0.08017091313318739
80-81	0.09266508141368412
82-83	0.09680765139321053
84-85	0.10081730468890185
86-87	0.08873296285022993
88-89	0.06810872407515463
90-91	0.09273153975560167
92-93	0.06834132827186601
94-95	0.09619844992563312
96-97	0.08473438594485819
98-99	0.07843191985301187
100-101	0.09490251225824117
102-103	0.06800903656227833
104-105	0.0742561207025267
106-107	0.0921887966299418
108-109	0.09893431833457168
110-111	0.09138022013661178
112-113	0.08080226738140399
114-115	0.09432653996162252
116-117	0.0879243863568999
118-119	0.08041459372021835
120-121	0.11363268828866668
122-123	0.09203372716546754
124-125	0.11368807024026463
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4514106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.87213139691797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.37302267321155	30.90544148673094
2	14.318036505663692	13.13597703867346
3	6.462329585992811	8.89322495696558
4	3.5846570498236083	6.577434368095876
5	2.2139359068453994	5.077897941158345
6	1.4386320525710814	3.9595871128415068
7	0.9841844332396426	3.1602646348259085
8	0.7032202356014267	2.5806568838784205
9	0.49906641775749433	2.060391626204484
>10	2.2959913202630946	17.43020822026903
>50	0.09061367738680941	2.821378237029467
>100	0.03485941485809854	2.729640437327342
>500	0.001063866497153636	0.32657289033476466
>1k	3.868602881037965E-4	0.34132416566484375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4305561278357224E-5	0.0
3	0.0	0.0	0.0	4.4305561278357224E-5	0.0
4	0.0	0.0	0.0	4.4305561278357224E-5	0.0
5	0.0	0.0	0.0	4.4305561278357224E-5	0.0
6	0.0	0.0	0.0	4.4305561278357224E-5	0.0
7	0.0	0.0	0.0	4.4305561278357224E-5	0.0
8	0.0	0.0	0.0	4.4305561278357224E-5	0.0
9	0.0	0.0	0.0	6.645834191753584E-5	0.0
10-11	0.0	0.0	0.0	1.2184029351548236E-4	0.0
12-13	0.0	0.0	0.0	1.3291668383507167E-4	0.0
14-15	0.0	0.0	0.0	1.5506946447425027E-4	1.1076390319589306E-5
16-17	0.0	0.0	0.0	1.772222451134289E-4	2.2152780639178612E-5
18-19	0.0	0.0	0.0	1.772222451134289E-4	2.2152780639178612E-5
20-21	0.0	0.0	0.0	1.993750257526075E-4	2.2152780639178612E-5
22-23	0.0	0.0	0.0	3.433680999072685E-4	2.2152780639178612E-5
24-25	0.0	0.0	0.0	6.645834191753583E-4	2.2152780639178612E-5
26-27	0.0	0.0	0.0	0.0012627084964331808	2.2152780639178612E-5
28-29	0.0	0.0	0.0	0.00366628519578406	2.2152780639178612E-5
30-31	0.0	0.0	0.0	0.011984654325795627	2.2152780639178612E-5
32-33	0.0	0.0	0.0	0.03421496969721136	2.2152780639178612E-5
34-35	0.0	0.0	0.0	0.07002493960044359	2.2152780639178612E-5
36-37	0.0	0.0	0.0	0.12982637093590627	2.2152780639178612E-5
38-39	0.0	0.0	0.0	0.23108673123759166	2.2152780639178612E-5
40-41	0.0	0.0	0.0	0.35071174668915617	2.2152780639178612E-5
42-43	0.0	0.0	0.0	0.46672585889653456	2.2152780639178612E-5
44-45	0.0	0.0	0.0	0.609843898215948	2.2152780639178612E-5
46-47	0.0	0.0	0.0	0.787874719822707	2.2152780639178612E-5
48-49	0.0	0.0	0.0	0.9784107861002821	2.2152780639178612E-5
50-51	0.0	0.0	0.0	1.181141958119725	2.2152780639178612E-5
52-53	0.0	0.0	0.0	1.3879603181670968	2.2152780639178612E-5
54-55	0.0	0.0	0.0	1.62358836943572	2.2152780639178612E-5
56-57	0.0	0.0	0.0	1.9082405242588454	2.2152780639178612E-5
58-59	0.0	0.0	0.0	2.2254572666215635	2.2152780639178612E-5
60-61	0.0	0.0	0.0	2.5615371016985424	4.4305561278357224E-5
62-63	0.0	0.0	0.0	2.907430618598677	4.4305561278357224E-5
64-65	0.0	0.0	0.0	3.2770497635633724	4.4305561278357224E-5
66-67	0.0	0.0	0.0	3.716051417489975	4.4305561278357224E-5
68-69	0.0	0.0	0.0	4.210224571598452	4.4305561278357224E-5
70-71	0.0	0.0	0.0	4.750896412268565	4.4305561278357224E-5
72-73	0.0	0.0	0.0	5.295411760379574	4.4305561278357224E-5
74-75	1.1076390319589306E-5	0.0	0.0	5.861282389026753	4.4305561278357224E-5
76-77	2.2152780639178612E-5	0.0	0.0	6.492093894117684	4.4305561278357224E-5
78-79	2.2152780639178612E-5	0.0	0.0	7.200229680029667	4.4305561278357224E-5
80-81	2.2152780639178612E-5	2.2152780639178612E-5	0.0	7.954775541380729	4.4305561278357224E-5
82-83	2.2152780639178612E-5	2.2152780639178612E-5	0.0	8.732437829328775	4.4305561278357224E-5
84-85	2.2152780639178612E-5	2.2152780639178612E-5	0.0	9.517222236252316	4.4305561278357224E-5
86-87	2.2152780639178612E-5	2.2152780639178612E-5	0.0	10.349657274330731	4.4305561278357224E-5
88-89	2.2152780639178612E-5	2.2152780639178612E-5	0.0	11.258630169517508	4.4305561278357224E-5
90-91	2.2152780639178612E-5	2.2152780639178612E-5	0.0	12.222774564886159	4.4305561278357224E-5
92-93	2.2152780639178612E-5	2.2152780639178612E-5	0.0	13.211608234277175	4.4305561278357224E-5
94-95	2.2152780639178612E-5	2.2152780639178612E-5	0.0	14.184658047462776	4.4305561278357224E-5
96-97	2.2152780639178612E-5	2.2152780639178612E-5	0.0	15.18377060707037	6.645834191753584E-5
98-99	2.2152780639178612E-5	3.322917095876792E-5	0.0	16.24607397345122	6.645834191753584E-5
100-101	2.2152780639178612E-5	6.645834191753584E-5	0.0	17.383519571760168	6.645834191753584E-5
102-103	2.2152780639178612E-5	6.645834191753584E-5	0.0	18.529516143395835	6.645834191753584E-5
104-105	2.2152780639178612E-5	6.645834191753584E-5	0.0	19.66096941454188	6.645834191753584E-5
106-107	2.2152780639178612E-5	6.645834191753584E-5	0.0	20.779839906284877	6.645834191753584E-5
108-109	2.2152780639178612E-5	6.645834191753584E-5	0.0	21.93762175722059	6.645834191753584E-5
110-111	2.2152780639178612E-5	6.645834191753584E-5	0.0	23.138679065134937	6.645834191753584E-5
112-113	2.2152780639178612E-5	6.645834191753584E-5	0.0	24.365333911077855	6.645834191753584E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10585	0.0	34.946278	1
GGTATCA	7670	0.0	32.437008	1
GTACATG	16715	0.0	31.481998	1
TACATGG	17150	0.0	30.1955	2
TCAACGC	11965	0.0	29.998625	4
ATCAACG	12230	0.0	29.543299	3
AACGCAG	12275	0.0	29.478905	6
CAACGCA	12180	0.0	29.468765	5
TATCAAC	12540	0.0	29.049656	2
CATGGGG	11395	0.0	28.887339	4
ACATGGG	17540	0.0	28.472729	3
ACGCAGA	13745	0.0	25.761015	7
CGCAGAG	13890	0.0	25.106775	8
TATACCG	435	0.0	21.893875	5
GCAGAGT	16245	0.0	20.771072	9
ATGGGGA	6565	0.0	20.672453	5
AGAGTAC	13540	0.0	19.622313	10-11
ATGGGGG	5900	0.0	19.269625	5
CAGAGTA	15120	0.0	18.339249	10-11
GTATAGA	1510	0.0	18.175274	1
>>END_MODULE