FastQCFastQC Report
Mon 15 Aug 2016
SRR2921085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514106
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.17365564743052112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.15123703342367237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.10343133280432493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89100.045.065121
GTATCAA137150.043.849831
TCAACGC171150.034.4439284
ATCAACG172900.034.3021473
TATCAAC175700.033.8572772
CAACGCA174000.033.7769855
AACGCAG180050.032.7635426
ACGCAGA202550.028.8419657
CGCAGAG205650.028.2336278
GTACATG165900.028.0152341
TACATGG167300.027.4339312
ACATGGG169200.026.315613
CATGGGG110400.025.3219414
GCAGAGT230150.024.5288289
GAGTACT125350.024.24995212-13
AGAGTAC204450.022.9263110-11
GTACTTT138850.022.1081214-15
AGTACTT137850.022.0510112-13
CAGAGTA220250.021.21413410-11
GTATAGT12650.018.3940471
Produced by FastQC (version 0.11.5)