FastQCFastQC Report
Mon 15 Aug 2016
SRR2921084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8523776
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146700.045.013791
GGTATCA90950.041.3861241
TCAACGC167650.038.308114
CAACGCA176750.036.3355985
ATCAACG179900.035.765753
AACGCAG183750.035.011856
TATCAAC186350.034.878962
GTACATG316100.033.7042771
TACATGG326650.031.7785512
ACATGGG327550.030.5829493
CATGGGG245700.029.4820884
ACGCAGA216000.029.4788847
CGCAGAG230850.027.582588
GCAGAGT258000.023.6420529
AGAGTAC220450.020.89138410-11
ATGGGGG134000.020.521425
CAGAGTA239750.019.84249310-11
ATGGGGA125600.019.0979025
GTATATA31300.019.0582831
GTATTAC21850.018.291591
Produced by FastQC (version 0.11.5)