FastQCFastQC Report
Mon 15 Aug 2016
SRR2921084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8523776
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97010.11381106213959634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104750.056.4395521
GTATCAA183150.048.76171
TCAACGC229500.038.591934
CAACGCA237500.037.1916125
TATCAAC239700.036.875992
ATCAACG239950.036.811873
AACGCAG246300.036.0356836
GTACATG308850.032.8371351
TACATGG324300.030.6930582
ACGCAGA290100.030.4309257
ACATGGG322400.029.8009683
CATGGGG246500.028.724994
CGCAGAG308100.028.5568648
GCAGAGT339700.025.3919669
AGAGTAC303300.023.4574810-11
CAGAGTA323050.021.93131310-11
TATACCG8350.021.4138725
GAGTACT176500.020.9643312-13
GTACTTT183050.020.15035814-15
ATGGGGA130950.019.799045
Produced by FastQC (version 0.11.5)