FastQCFastQC Report
Mon 15 Aug 2016
SRR2921083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054575
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94000.033.255131
TACATGG148850.029.8687882
GTACATG153400.029.633631
GGTATCA68500.029.4361741
AACGCAG108400.027.5598856
ACATGGG159350.027.3787653
CATGGGG99350.027.3785384
TCAACGC109800.027.212184
CAACGCA111750.026.9500485
ATCAACG113500.026.3250873
TATCAAC116550.026.0948282
ACGCAGA123600.023.7831027
CGCAGAG125300.023.3179578
ACGGCGT2204.1781404E-621.638518
GTATAGG9200.021.3984011
ATGGGGC49150.020.1020855
GTATAAT21000.019.8853861
GTATTAC9300.019.2439211
GCAGAGT142950.019.1898449
ATGGGGA49000.018.4630765
Produced by FastQC (version 0.11.5)