##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921083_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4054575
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.17361055104419	31.0	30.0	34.0	22.0	34.0
2	29.297346824266416	31.0	30.0	34.0	22.0	34.0
3	29.376212056750706	31.0	30.0	34.0	22.0	34.0
4	32.68415308632841	37.0	35.0	37.0	23.0	37.0
5	32.77975718786803	37.0	35.0	37.0	25.0	37.0
6	32.72438837609367	37.0	35.0	37.0	23.0	37.0
7	32.65230733184119	37.0	35.0	37.0	23.0	37.0
8	32.594226275256965	37.0	35.0	37.0	22.0	37.0
9	33.947577489625914	39.0	35.0	39.0	19.0	39.0
10-11	33.90281891443616	39.0	35.0	39.0	20.0	39.0
12-13	33.816141889100585	39.0	35.0	39.0	18.5	39.0
14-15	34.944686557777324	40.0	35.0	41.0	18.5	41.0
16-17	34.90335756521954	40.0	35.0	41.0	19.0	41.0
18-19	34.921343174068795	40.0	35.0	41.0	19.0	41.0
20-21	34.957669176177525	40.0	35.0	41.0	19.0	41.0
22-23	34.86680724860189	40.0	35.0	41.0	19.0	41.0
24-25	34.83949649470043	40.0	34.5	41.0	19.5	41.0
26-27	34.763503819759165	40.0	34.0	41.0	19.0	41.0
28-29	34.62774803771049	40.0	34.0	41.0	18.0	41.0
30-31	34.521692779144544	40.0	34.0	41.0	17.5	41.0
32-33	34.39924159745473	40.0	34.0	41.0	16.5	41.0
34-35	34.27885586035528	39.0	34.0	41.0	16.0	41.0
36-37	34.15353804036181	39.0	34.0	41.0	15.0	41.0
38-39	34.012797765487136	39.0	33.5	41.0	11.0	41.0
40-41	33.88621421480673	39.0	33.0	41.0	9.0	41.0
42-43	33.69616322302584	39.0	33.0	41.0	8.5	41.0
44-45	33.506991484927525	39.0	33.0	41.0	7.5	41.0
46-47	33.33738801230709	38.5	33.0	41.0	2.0	41.0
48-49	33.10744886948694	38.0	32.5	40.0	2.0	41.0
50-51	32.944933439386375	38.0	32.0	40.0	2.0	41.0
52-53	32.76791944901747	38.0	32.0	40.0	2.0	41.0
54-55	32.54576533915392	38.0	32.0	40.0	2.0	41.0
56-57	32.310917173809834	38.0	31.0	40.0	2.0	41.0
58-59	32.03776820011962	37.0	31.0	40.0	2.0	41.0
60-61	31.747585381945086	37.0	31.0	40.0	2.0	41.0
62-63	31.47513278210417	36.0	30.5	40.0	2.0	41.0
64-65	31.173077079595274	36.0	30.0	39.0	2.0	41.0
66-67	30.832828964811355	35.0	30.0	39.0	2.0	41.0
68-69	30.47759000635085	35.0	29.5	38.5	2.0	40.0
70-71	30.123642428614588	35.0	29.0	38.0	2.0	40.0
72-73	29.765175018343477	35.0	29.0	37.0	2.0	39.5
74-75	29.718095731365185	35.0	29.0	37.0	2.0	39.0
76-77	29.75170615909189	35.0	30.0	36.5	2.0	39.0
78-79	29.55983315144004	35.0	30.0	36.0	2.0	39.0
80-81	29.3283262734072	35.0	30.0	36.0	2.0	37.5
82-83	29.068502494096176	35.0	29.5	35.0	2.0	37.0
84-85	28.799954865799744	35.0	29.0	35.0	2.0	37.0
86-87	28.635818057379627	35.0	29.0	35.0	2.0	36.0
88-89	28.446869277297868	34.0	29.0	35.0	2.0	36.0
90-91	28.291275287792185	34.0	29.0	35.0	2.0	36.0
92-93	28.17041206538293	34.0	29.0	35.0	2.0	35.0
94-95	28.048007374385726	34.0	29.0	35.0	2.0	35.0
96-97	27.943212173902317	34.0	29.0	35.0	2.0	35.0
98-99	27.838795804739092	34.0	29.0	35.0	2.0	35.0
100-101	27.70994197912235	34.0	28.0	35.0	2.0	35.0
102-103	26.444824673362806	32.5	24.5	34.5	2.0	35.0
104-105	27.384176886603406	34.0	27.0	35.0	2.0	35.0
106-107	27.529852573944247	34.0	27.0	35.0	2.0	35.0
108-109	27.471581115160035	34.0	27.0	35.0	2.0	35.0
110-111	27.378924178243096	34.0	27.0	35.0	2.0	35.0
112-113	27.2715272254182	34.0	27.0	35.0	2.0	35.0
114-115	27.141359575294572	34.0	27.0	35.0	2.0	35.0
116-117	27.004472971889776	34.0	26.0	35.0	2.0	35.0
118-119	26.80262715574382	34.0	25.0	35.0	2.0	35.0
120-121	26.60131641417411	34.0	25.0	35.0	2.0	35.0
122-123	26.314931774600296	34.0	24.5	35.0	2.0	35.0
124-125	25.085640788492015	32.5	19.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	221704.0
3	29608.0
4	18951.0
5	18507.0
6	24014.0
7	24034.0
8	22700.0
9	23855.0
10	25528.0
11	26391.0
12	27006.0
13	26043.0
14	25554.0
15	22813.0
16	19068.0
17	17507.0
18	16756.0
19	16536.0
20	16902.0
21	17941.0
22	21442.0
23	27487.0
24	35972.0
25	44619.0
26	50374.0
27	56442.0
28	64600.0
29	75677.0
30	88641.0
31	107108.0
32	132058.0
33	168884.0
34	228869.0
35	338425.0
36	616241.0
37	1004463.0
38	351444.0
39	411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20115052846341	18.51444987760546	12.19262842521664	25.091771168714494
2	15.989729507328821	20.832252486482524	38.5456684224309	24.632349583757755
3	18.77219701719482	25.093132439117106	29.321315554326844	26.813354989361233
4	12.81543327270537	16.983827935074757	36.58011383048264	33.62062496173724
5	13.619290095759725	38.23787684917442	34.313947434232304	13.828885620833548
6	32.039503172774516	37.268240520880795	17.118930579273677	13.573325727071008
7	27.751192745912096	33.13375422162048	21.13028926418794	17.984763768279493
8	25.76809612316503	35.677396047777115	19.976586041359393	18.57792178769846
9	26.106844290218234	16.321324580567843	19.295817741759834	38.276013387454086
10-11	24.70286008089479	26.68270242678206	27.878051686007723	20.736385806315425
12-13	25.211464819719204	24.42439651307695	27.527394642825403	22.83674402437844
14-15	22.800866289302803	25.912809640776825	24.941579541075047	26.344744528845325
16-17	22.780804168386705	28.366186076276893	25.793016115365397	23.059993639971005
18-19	22.784679969451478	27.725089495879075	27.11687178620579	22.373358748463655
20-21	24.11846246898646	26.88865984471628	26.59959512670806	22.3932825595892
22-23	23.587073671100068	26.745335661907948	26.33998250730518	23.327608159686804
24-25	23.101848246109594	26.794600550569758	26.73995829809935	23.363592905221303
26-27	23.10233736427632	26.928005190576677	26.654033399524046	23.315624045622958
28-29	23.146466904470184	27.10416983999982	26.624682800406745	23.124680455123247
30-31	23.41340960303025	27.010485108450293	26.488969825875213	23.08713546264424
32-33	23.068545212465892	27.109889152202847	26.501669296377315	23.319896338953942
34-35	23.28092145500822	27.031778545670665	26.571927418692876	23.11537258062824
36-37	23.37729392124371	26.852050372921315	26.500390356196174	23.270265349638805
38-39	23.173755339908393	27.03015254800626	26.55899777264434	23.237094339441004
40-41	23.289309532329455	26.96689765820962	26.67229611090655	23.07149669855437
42-43	23.134606424699083	26.910312780590758	26.649239570123388	23.30584122458677
44-45	23.174937967557387	26.963813534272578	26.540974734699844	23.32027376347019
46-47	23.05287250335876	26.97096306172307	26.489229199906163	23.48693523501201
48-49	22.977421440406985	26.885986178235626	26.593747466312212	23.542844915045176
50-51	22.831145969830153	27.055526444460842	26.511731743744154	23.601595841964848
52-53	23.018687211223313	26.94051454783441	26.369252776915513	23.671545464026764
54-55	23.09386362287029	26.84561157208105	26.405663448016742	23.654861357031916
56-57	23.006086198264107	26.95938699550374	26.36365454331787	23.670872262914287
58-59	22.88833186646007	26.668409966971907	26.53214624372671	23.911111922841314
60-61	22.98652950886484	26.656534196592794	26.511372615621365	23.845563678920996
62-63	22.826192008221586	26.559404739877955	26.535014498986065	24.079388752914394
64-65	22.854922687785827	26.504373673685954	26.545744427969098	24.094959210559118
66-67	22.79843249808184	26.637585465520747	26.444874993659624	24.119107042737788
68-69	22.970452469699588	26.434931253239863	26.489842265063814	24.104774011996742
70-71	22.778497806610197	26.17916223531124	26.724205068215824	24.31813488986274
72-73	22.79056876623536	26.13917922110743	26.79617647882689	24.274075533830324
74-75	22.611059995887192	26.327337011489938	26.78594219703409	24.27566079558878
76-77	22.562774564076584	26.110794493344297	26.969168095149747	24.357262847429368
78-79	22.575404383125058	26.095879996722086	26.95023592101015	24.37847969914271
80-81	22.61393610468927	26.019509509744264	26.985172229497795	24.381382156068675
82-83	22.580124735443892	25.986143468719735	27.086928947011753	24.346802848824623
84-85	22.65867456593934	25.892670768136128	26.965698921824334	24.482955744100202
86-87	22.43059404070862	25.73166773019401	27.160419009203935	24.677319219893434
88-89	22.62530126860134	25.577662606067957	27.116685335211333	24.680350790119366
90-91	22.601879341621917	25.64748711592318	27.171469772825763	24.57916376962914
92-93	22.593941461099504	25.62669083030249	27.153486761561506	24.625880947036496
94-95	22.34646456754048	25.613973239838046	27.235414845215704	24.804147347405767
96-97	22.40284945476124	25.48308990928773	27.260456940615295	24.85360369533573
98-99	22.318394465731263	25.556401762694307	27.14235520139092	24.982848570183503
100-101	22.27193443599898	25.738136268025784	27.208082829898938	24.7818464660763
102-103	22.07816711623558	25.744410971348973	27.31156143825362	24.86586047416183
104-105	21.89757862654693	25.81749383053212	27.183273396188252	25.101654146732695
106-107	21.76246358960712	25.927914982203205	27.257654190044878	25.051967238144794
108-109	21.770521088282884	25.938629914596167	27.237092033403133	25.053756963717817
110-111	21.71814749255692	26.1843667452951	27.157037203620572	24.94044855852741
112-113	21.72113307775405	26.28423899917052	27.13568998939728	24.858937933678153
114-115	21.651416324767787	26.300195999119673	27.12402427826617	24.924363397846367
116-117	21.540186257929737	26.229835778337907	27.215926047796508	25.01405191593585
118-119	21.61178702677179	26.179120440654692	27.09030145738362	25.118791075189893
120-121	21.61294265213505	26.309623162354757	27.106471079155163	24.97096310635503
122-123	21.678124622768735	26.37680000256733	27.006068038757835	24.939007335906098
124-125	21.65486647984392	26.46850072956673	26.84874703287513	25.027885757714213
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2844.0
1	2082.5
2	1108.0
3	798.0
4	611.5
5	516.0
6	502.5
7	533.5
8	585.0
9	633.0
10	655.5
11	654.5
12	698.5
13	793.0
14	943.5
15	1127.0
16	1305.5
17	1569.0
18	1900.0
19	2289.0
20	2789.5
21	3479.5
22	4455.0
23	5769.5
24	7416.5
25	9612.0
26	13124.0
27	17964.0
28	22062.0
29	26592.0
30	32712.0
31	39055.5
32	46481.0
33	55039.0
34	64946.5
35	74990.5
36	86552.0
37	96968.0
38	102598.5
39	107406.5
40	115588.5
41	123952.5
42	129845.5
43	137861.0
44	145683.0
45	151607.5
46	157489.5
47	161476.5
48	163913.0
49	167077.5
50	170813.0
51	170619.0
52	163451.0
53	155593.0
54	148297.0
55	139825.5
56	129535.5
57	116435.5
58	105103.0
59	94091.5
60	80257.5
61	66403.5
62	53580.5
63	40399.0
64	29841.5
65	22832.0
66	16998.5
67	12544.5
68	9478.0
69	7305.5
70	5732.5
71	4516.0
72	3506.0
73	2855.0
74	2262.0
75	1692.5
76	1250.0
77	897.5
78	643.0
79	501.0
80	374.5
81	277.5
82	219.5
83	183.0
84	141.5
85	98.5
86	84.0
87	74.0
88	69.5
89	55.0
90	40.0
91	39.0
92	33.0
93	27.5
94	28.5
95	30.5
96	33.5
97	37.0
98	40.0
99	50.5
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29376691761775275
2	0.0732012603047175
3	0.0827460338013232
4	0.08975046706498215
5	0.09658225584679035
6	0.13022326631027914
7	0.1205058483318227
8	0.1197166164147907
9	0.12607979874586114
10-11	0.10488152272432993
12-13	0.09330201069162612
14-15	0.1034880351208203
16-17	0.10553510540562204
18-19	0.09790175295807822
20-21	0.0968042273234556
22-23	0.07817095503227836
24-25	0.09077400220738302
26-27	0.10255082221934482
28-29	0.09599033190901636
30-31	0.09093431494053014
32-33	0.10028178045787782
34-35	0.09390626637810374
36-37	0.09341299642995875
38-39	0.08283235604224858
40-41	0.09494213326920824
42-43	0.08416418490224006
44-45	0.08058797777818884
46-47	0.07045128034380915
48-49	0.07205440767528039
50-51	0.075125013102483
52-53	0.08737043956518255
54-55	0.092648428010334
56-57	0.10109567587231708
58-59	0.07085822805102877
60-61	0.08809801273869641
62-63	0.0930923709636645
64-65	0.08395454517427844
66-67	0.07794898355561311
68-69	0.08570565349019318
70-71	0.08448481036853431
72-73	0.06549391736495193
74-75	0.09406657911125088
76-77	0.09740848300993322
78-79	0.07939179815393722
80-81	0.09199484532904188
82-83	0.09673023683123384
84-85	0.1005160836832467
86-87	0.0885419556920269
88-89	0.06783694961864067
90-91	0.09227847554922526
92-93	0.06798493060308416
94-95	0.09592867316549823
96-97	0.08420118014835094
98-99	0.07802297404783486
100-101	0.09410357435736175
102-103	0.06734367967049568
104-105	0.07323825555082839
106-107	0.09139058964256426
108-109	0.0971001892923426
110-111	0.09040404974627426
112-113	0.08029201580930184
114-115	0.09351165041958776
116-117	0.08696349185796293
118-119	0.07989739985078584
120-121	0.11234223069002301
122-123	0.09071234346386489
124-125	0.11267518790502087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4054575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.999196170491146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40914455391194	34.499914861936
2	13.333947340131639	13.067054028922179
3	6.016571512785733	8.844215034863305
4	3.2292303867112717	6.3291877279270645
5	1.948232950992516	4.773092427574858
6	1.2274307401014337	3.608587177195276
7	0.8206061973651719	2.8146330830391784
8	0.5826879250361867	2.284099195601964
9	0.43871356253912064	1.9346950722158467
>10	1.871935197619322	15.292187460920248
>50	0.08599429666181947	2.877774688358482
>100	0.03404190063059889	2.9240572655756316
>500	9.083393389262913E-4	0.2917492590886331
>1k	5.550961743992976E-4	0.458752716781326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.865398962899935E-5
2	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
3	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
4	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
5	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
6	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
7	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
8	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
9	0.0	0.0	0.0	4.932699481449967E-5	9.865398962899935E-5
10-11	0.0	0.0	0.0	9.865398962899935E-5	9.865398962899935E-5
12-13	0.0	0.0	0.0	9.865398962899935E-5	2.4663497407249836E-4
14-15	0.0	0.0	0.0	1.2331748703624918E-4	3.2062546629424784E-4
16-17	0.0	0.0	0.0	1.6031273314712392E-4	3.8228420981237243E-4
18-19	0.0	0.0	0.0	2.219714766652485E-4	4.43942953330497E-4
20-21	0.0	0.0	0.0	3.2062546629424784E-4	4.932699481449967E-4
22-23	0.0	0.0	0.0	6.04255686477621E-4	5.91923937773996E-4
24-25	0.0	0.0	0.0	0.001171516126844367	5.91923937773996E-4
26-27	0.0	0.0	0.0	0.0023553640023923593	6.659144299957456E-4
28-29	0.0	0.0	0.0	0.0065974855564393305	6.659144299957456E-4
30-31	0.0	0.0	0.0	0.023331668547258343	6.659144299957456E-4
32-33	0.0	0.0	0.0	0.06611050480013318	6.659144299957456E-4
34-35	0.0	0.0	0.0	0.13503264830469283	7.399049222174951E-4
36-37	0.0	0.0	0.0	0.24652398833416572	7.399049222174951E-4
38-39	0.0	0.0	0.0	0.42579061923876116	7.5223667092112E-4
40-41	0.0	0.0	0.0	0.6286602171620947	7.645684196247449E-4
42-43	0.0	0.0	0.0	0.822034368583637	7.645684196247449E-4
44-45	0.0	0.0	0.0	1.0564609114395467	8.138954144392446E-4
46-47	0.0	0.0	0.0	1.3334813143177768	8.87885906660994E-4
48-49	0.0	0.0	0.0	1.6208110591122376	9.372129014754937E-4
50-51	0.0	0.0	0.0	1.9181541838540415	9.618763988827435E-4
52-53	0.0	0.0	0.0	2.2112428552930945	0.0010112033936972432
54-55	0.0	0.0	0.0	2.531979307325675	0.0010605303885117428
56-57	0.0	0.0	0.0	2.9098980780969645	0.001072862137215368
58-59	0.0	0.0	0.0	3.323961204318578	0.0010851938859189928
60-61	0.0	0.0	0.0	3.757446834748402	0.0010851938859189928
62-63	0.0	0.0	0.0	4.187750874012689	0.0011345208807334924
64-65	0.0	0.0	0.0	4.641485235813864	0.0011591843781407422
66-67	0.0	0.0	0.0	5.160762841974806	0.001208511372955242
68-69	0.0	0.0	0.0	5.743203664995715	0.0012331748703624918
70-71	0.0	0.0	0.0	6.3774501643205515	0.0012701701164733665
72-73	0.0	0.0	0.0	7.006578987933384	0.001344160608695116
74-75	0.0	0.0	0.0	7.656745774834601	0.0014428145983241152
76-77	0.0	0.0	0.0	8.366141950759328	0.0014674780957313653
78-79	0.0	0.0	0.0	9.150835784268388	0.0014798098444349901
80-81	0.0	0.0	0.0	9.98216829137456	0.001492141593138615
82-83	0.0	1.2331748703624918E-5	0.0	10.835426647675774	0.0015291368392494897
84-85	0.0	2.4663497407249836E-5	0.0	11.677228809431321	0.0015291368392494897
86-87	0.0	2.4663497407249836E-5	0.0	12.566162914732123	0.0016031273314712391
88-89	0.0	2.4663497407249836E-5	0.0	13.528063977112275	0.0016771178236929887
90-91	0.0	2.4663497407249836E-5	0.0	14.53199163907438	0.0016771178236929887
92-93	0.0	2.4663497407249836E-5	0.0	15.563160133922791	0.0018004353107292379
94-95	0.0	2.4663497407249836E-5	0.0	16.58282310723072	0.0018374305568401128
96-97	0.0	2.4663497407249836E-5	0.0	17.603423293440127	0.001936084546469112
98-99	0.0	2.4663497407249836E-5	0.0	18.695177669669448	0.0020224067873944864
100-101	0.0	2.4663497407249836E-5	0.0	19.855669213172774	0.0020224067873944864
102-103	0.0	2.4663497407249836E-5	0.0	21.02931873254287	0.002059402033505361
104-105	0.0	2.4663497407249836E-5	0.0	22.168316038055778	0.002071733782208986
106-107	0.0	2.4663497407249836E-5	0.0	23.30495797956629	0.002084065530912611
108-109	0.0	2.4663497407249836E-5	0.0	24.463673751256298	0.0021210607770234856
110-111	0.0	4.932699481449967E-5	0.0	25.660901574147722	0.0021210607770234856
112-113	0.0	4.932699481449967E-5	0.0	26.88725698747711	0.0021210607770234856
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9400	0.0	33.25513	1
TACATGG	14885	0.0	29.868788	2
GTACATG	15340	0.0	29.63363	1
GGTATCA	6850	0.0	29.436174	1
AACGCAG	10840	0.0	27.559885	6
ACATGGG	15935	0.0	27.378765	3
CATGGGG	9935	0.0	27.378538	4
TCAACGC	10980	0.0	27.21218	4
CAACGCA	11175	0.0	26.950048	5
ATCAACG	11350	0.0	26.325087	3
TATCAAC	11655	0.0	26.094828	2
ACGCAGA	12360	0.0	23.783102	7
CGCAGAG	12530	0.0	23.317957	8
ACGGCGT	220	4.1781404E-6	21.63851	8
GTATAGG	920	0.0	21.398401	1
ATGGGGC	4915	0.0	20.102085	5
GTATAAT	2100	0.0	19.885386	1
GTATTAC	930	0.0	19.243921	1
GCAGAGT	14295	0.0	19.189844	9
ATGGGGA	4900	0.0	18.463076	5
>>END_MODULE