FastQCFastQC Report
Mon 15 Aug 2016
SRR2921083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054575
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT95550.23565971772627217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81210.20029226244427592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.16753913788744812No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT60800.149954064236079TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.11611574579333223No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT40840.10072572341120833RNA PCR Primer, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86850.044.241291
GTATCAA131450.042.938051
TCAACGC166600.033.1647074
ATCAACG166650.033.118993
CAACGCA169050.032.64885
TATCAAC171600.032.5808452
AACGCAG175800.031.4222556
ACGCAGA196550.027.8757697
CGCAGAG201400.027.2642338
GTACATG159100.027.1881451
TACATGG157900.026.8388712
ACATGGG164500.025.1457713
CATGGGG98900.024.829754
GCAGAGT223750.024.0622659
GAGTACT117600.023.34543812-13
AGTACTT124600.023.20362512-13
AGAGTAC199950.022.41799510-11
CAGAGTA208650.021.39770710-11
GTACTTT130750.021.02154714-15
GTATAAT19150.020.874581
Produced by FastQC (version 0.11.5)