FastQCFastQC Report
Mon 15 Aug 2016
SRR2921082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705729
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103700.031.9813231
GGTATCA77300.030.0943891
GTACATG161400.028.4938561
TCAACGC119050.026.9501424
TACATGG167350.026.9256342
AACGCAG121700.026.4557696
TATCAAC125350.026.1176552
CAACGCA123000.026.0843165
ACATGGG168200.026.011363
CATGGGG109800.025.8051454
ATCAACG129650.024.9303863
ACGCAGA132850.023.9656017
CGCAGAG138300.022.8487558
GTACTAG6300.019.8828741
GTATAGG5700.019.8828741
GCAGAGT149150.019.869939
AGAGTAC134050.018.17734310-11
ATGGGGC48700.018.0895825
GAGTACT50450.017.51493612-13
ATGGGGA52900.017.441025
Produced by FastQC (version 0.11.5)