##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921082_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3705729
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.727459023582135	31.0	30.0	34.0	25.0	34.0
2	29.831260191989216	31.0	31.0	34.0	25.0	34.0
3	29.915935299100394	31.0	31.0	34.0	25.0	34.0
4	33.249398701308166	37.0	35.0	37.0	26.0	37.0
5	33.34951476484114	37.0	35.0	37.0	27.0	37.0
6	33.29345966745005	37.0	35.0	37.0	26.0	37.0
7	33.24189491460385	37.0	35.0	37.0	26.0	37.0
8	33.188385335247126	37.0	35.0	37.0	26.0	37.0
9	34.59224919037523	39.0	35.0	39.0	26.0	39.0
10-11	34.55976597317289	39.0	35.0	39.0	26.0	39.0
12-13	34.489619181542956	39.0	35.0	39.0	25.0	39.0
14-15	35.681534591439366	40.0	36.0	41.0	25.0	41.0
16-17	35.65391789307853	40.0	36.0	41.0	25.0	41.0
18-19	35.673335260079725	40.0	36.0	41.0	25.0	41.0
20-21	35.71908091498326	40.0	36.0	41.0	25.5	41.0
22-23	35.64777767073631	40.0	36.0	41.0	25.0	41.0
24-25	35.62649683773422	40.0	36.0	41.0	25.0	41.0
26-27	35.56119632601305	40.0	36.0	41.0	25.0	41.0
28-29	35.431728008173295	40.0	35.5	41.0	25.0	41.0
30-31	35.32080300529262	40.0	35.0	41.0	24.5	41.0
32-33	35.196041858430554	40.0	35.0	41.0	24.0	41.0
34-35	35.08164331498607	40.0	35.0	41.0	24.0	41.0
36-37	34.951935503108835	40.0	35.0	41.0	23.5	41.0
38-39	34.81376120595975	40.0	35.0	41.0	22.5	41.0
40-41	34.67665228623032	40.0	35.0	41.0	21.5	41.0
42-43	34.48097783189219	39.0	34.0	41.0	19.5	41.0
44-45	34.28585468608201	39.0	34.0	41.0	18.0	41.0
46-47	34.09440571612225	39.0	33.5	41.0	16.0	41.0
48-49	33.86308294535299	39.0	33.0	41.0	14.0	41.0
50-51	33.675783091532054	38.5	33.0	40.5	12.0	41.0
52-53	33.484726352088884	38.0	33.0	40.0	8.5	41.0
54-55	33.24590802511463	38.0	33.0	40.0	8.0	41.0
56-57	32.99015740222774	38.0	32.5	40.0	7.0	41.0
58-59	32.704878176466764	37.0	32.0	40.0	3.5	41.0
60-61	32.392682789270346	37.0	32.0	40.0	2.0	41.0
62-63	32.09575767682958	36.0	31.0	39.5	2.0	41.0
64-65	31.76763667823524	36.0	31.0	39.0	2.0	41.0
66-67	31.415812516241743	35.0	31.0	39.0	2.0	40.5
68-69	31.053544795099697	35.0	30.5	38.5	2.0	40.0
70-71	30.68753233169506	35.0	30.0	37.5	2.0	40.0
72-73	30.335429951839437	35.0	30.0	37.0	2.0	39.0
74-75	30.284898463972944	35.0	30.0	36.5	2.0	39.0
76-77	30.332049645292464	35.0	31.0	36.0	2.0	39.0
78-79	30.155198477816377	35.0	31.0	36.0	2.0	38.0
80-81	29.948367244339778	35.0	31.0	35.5	2.0	37.0
82-83	29.7144359449922	35.0	31.0	35.0	2.0	37.0
84-85	29.473996344578893	35.0	30.5	35.0	2.0	36.0
86-87	29.333183025526154	35.0	30.0	35.0	2.0	36.0
88-89	29.16680806934344	35.0	30.0	35.0	2.0	36.0
90-91	29.031242030920232	35.0	30.0	35.0	2.0	35.5
92-93	28.918322683606924	35.0	30.0	35.0	2.0	35.0
94-95	28.808100511397353	35.0	30.0	35.0	2.0	35.0
96-97	28.709183267313932	35.0	30.0	35.0	2.0	35.0
98-99	28.605203996298705	34.5	29.0	35.0	2.0	35.0
100-101	28.473602629873906	34.0	29.0	35.0	2.0	35.0
102-103	27.19159981747181	32.5	26.0	34.5	2.0	35.0
104-105	28.132316880160424	34.0	29.0	35.0	2.0	35.0
106-107	28.265383410389695	34.0	29.0	35.0	2.0	35.0
108-109	28.195370195715878	34.0	29.0	35.0	2.0	35.0
110-111	28.0891346884783	34.0	29.0	35.0	2.0	35.0
112-113	27.97457463835051	34.0	29.0	35.0	2.0	35.0
114-115	27.823979168471304	34.0	29.0	35.0	2.0	35.0
116-117	27.680685770600064	34.0	29.0	35.0	2.0	35.0
118-119	27.472369809017337	34.0	27.5	35.0	2.0	35.0
120-121	27.25535218576426	34.0	27.0	35.0	2.0	35.0
122-123	26.954084742840074	34.0	27.0	35.0	2.0	35.0
124-125	25.726157660206667	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	160595.0
3	22028.0
4	14441.0
5	15124.0
6	20739.0
7	20785.0
8	18278.0
9	19377.0
10	20409.0
11	21480.0
12	21997.0
13	22493.0
14	22411.0
15	19764.0
16	15406.0
17	13864.0
18	13150.0
19	13125.0
20	13396.0
21	14617.0
22	17455.0
23	22833.0
24	31401.0
25	40273.0
26	45310.0
27	49213.0
28	56997.0
29	67087.0
30	79825.0
31	97136.0
32	121033.0
33	157026.0
34	214609.0
35	324952.0
36	613687.0
37	960405.0
38	302667.0
39	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.526023206317106	17.399351963919568	11.74319107429356	25.331433755469767
2	16.354511121343013	20.20731755069855	38.139840103267204	25.298331224691236
3	19.218704214092707	24.584147492569603	28.48454976712908	27.712598526208616
4	13.082628420455874	16.359740681010795	35.0987598268938	35.45887107163953
5	14.15726142782438	37.714089382722456	33.55255481077156	14.576094378681603
6	33.36393760834886	35.88646660585441	16.61685140912962	14.132744376667114
7	28.740804500347572	32.436160018241736	20.416717660843737	18.406317820566958
8	26.74622204271081	35.08316436596374	19.340844895384322	18.829768695941134
9	26.66764113698531	16.022237376646647	18.37979809551394	38.9303233908541
10-11	25.460877979100776	26.110899211119744	26.90063036945874	21.52759244032074
12-13	26.00355099699771	23.711676438188224	26.828299558076846	23.456473006737223
14-15	23.49981726089067	24.938945148283402	24.0637018000275	27.497535790798434
16-17	23.70776056132006	27.579457829478326	24.95194043409677	23.76084117510484
18-19	23.8168424507171	26.77329631052699	26.29239598646489	23.117465252291023
20-21	24.72781475521155	25.97131264217675	25.95590312722668	23.34496947538502
22-23	24.445488671041616	25.743052742445112	25.75424667152772	24.05721191498555
24-25	24.055096051173276	25.8659636062806	26.011703778508426	24.067236564037696
26-27	23.84253731746452	25.993619699515403	25.99702324676798	24.1668197362521
28-29	23.997485340154338	26.20885992183216	25.886667440962214	23.906987297051284
30-31	24.12908929075678	26.220684866185607	25.690159287317176	23.960066555740433
32-33	23.741095029747612	26.260903810924795	25.768000505652665	24.23000065367492
34-35	24.0632584249785	26.210478508554726	25.87993334537067	23.846329721096104
36-37	24.213027711558645	25.997757102643344	25.738962292638686	24.050252893159325
38-39	24.04213475483959	26.147765208403833	25.701190389060642	24.108909647695935
40-41	24.057325978806958	26.166203958862877	25.848955264436263	23.927514797893902
42-43	24.02431429360047	26.076923243122952	25.75439992351482	24.14436253976176
44-45	23.879263618910066	26.196975757066728	25.816089452040625	24.107671171982584
46-47	23.93189073948181	26.192428508837367	25.756004825598627	24.1196759260822
48-49	23.91206133487223	26.322068029297768	25.471114458473902	24.2947561773561
50-51	23.805281450946815	26.361062663371875	25.583966477059988	24.24968940862132
52-53	23.993965813750204	26.215422501745746	25.455450476905167	24.335161207598883
54-55	23.79403695953919	26.243371480939388	25.497363447713127	24.46522811180829
56-57	23.85087956599551	26.179029283931214	25.512350281861707	24.457740868211573
58-59	23.87877103827483	25.941357062058103	25.59699189158755	24.58288000807952
60-61	23.841503449352462	26.000471563199596	25.718776020714213	24.43924896673373
62-63	23.726755551354035	25.975738464291382	25.809736350776024	24.48776963357856
64-65	23.766132664091515	25.90641012397043	25.741489819246645	24.58596739269141
66-67	23.790428698645723	26.016080672069197	25.6902543079498	24.50323632133528
68-69	23.98802251760209	25.876795977545058	25.680920873615115	24.454260631237737
70-71	23.704906034404765	25.739551163547535	25.839802737743018	24.715740064304686
72-73	23.842950407772417	25.708206280660562	25.851630380850864	24.597212930716157
74-75	23.667419929323046	25.866543537280563	25.966264294850404	24.49977223854599
76-77	23.793816304263125	25.573175542619413	26.023191323761647	24.609816829355815
78-79	23.759574544219625	25.648294119195945	26.042193147040017	24.549938189544413
80-81	23.719349923846632	25.678594164807283	26.08479047492746	24.517265436418622
82-83	23.691336349517186	25.53822675400095	26.170571949490174	24.599864946991694
84-85	23.619349274319827	25.619099146892598	26.03425395172973	24.727297627057844
86-87	23.62405980109336	25.54644229462955	26.11934614633572	24.71015175794137
88-89	23.65854880257263	25.424422881956293	26.047030838900564	24.869997476570518
90-91	23.561672251109652	25.563496203879726	26.070275795541725	24.804555749468893
92-93	23.654808737510546	25.486539007572894	26.19020005133295	24.668452203583612
94-95	23.44313756419682	25.39379723770665	26.332487549584233	24.830577648512296
96-97	23.50090319116743	25.301600492288813	26.266962432459838	24.930533884083918
98-99	23.385912329091934	25.532403009583422	26.14035819719358	24.941326464131063
100-101	23.34028841297237	25.606037279226868	26.212614758814045	24.841059548986717
102-103	23.148707326323198	25.651102791619966	26.32780064220207	24.872389239854765
104-105	23.123963023133225	25.90505936692562	26.09538782934817	24.87558978059298
106-107	23.080202611893537	25.897608825296132	26.13142709904366	24.890761463766676
108-109	23.06411061885471	25.88479201431787	26.205869117660562	24.845228249166855
110-111	23.10937119553834	26.103325110463167	26.13183304102914	24.655470652969356
112-113	23.07430347664167	26.246976994778354	26.07645992079085	24.602259607789126
114-115	23.1501498776184	26.38594256518961	26.02588785405177	24.438019703140217
116-117	22.987373422960758	26.313250708221037	26.065180912696988	24.63419495612122
118-119	23.152813326428774	26.26238989107766	25.94967278737357	24.635123995119997
120-121	23.16118443313213	26.3776115074563	25.97753527158489	24.48366878782668
122-123	23.176824566402562	26.44903179106836	25.968796379699416	24.405347262829665
124-125	23.293177084747395	26.587302391315475	25.771750580920937	24.34776994301619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3127.0
1	2279.5
2	1226.5
3	923.0
4	735.5
5	603.0
6	559.0
7	600.5
8	708.5
9	769.0
10	759.0
11	790.0
12	851.0
13	940.0
14	1094.5
15	1256.0
16	1449.5
17	1747.5
18	2106.0
19	2484.5
20	2962.5
21	3618.0
22	4546.0
23	5616.0
24	6894.0
25	8496.5
26	10719.0
27	13834.5
28	16968.0
29	20534.5
30	24664.5
31	29444.5
32	35469.0
33	40115.5
34	43518.5
35	48434.0
36	54642.5
37	61499.0
38	68334.0
39	75146.5
40	82621.0
41	90118.5
42	97324.5
43	105948.0
44	115134.5
45	124655.5
46	134924.0
47	143657.0
48	152472.5
49	160603.5
50	165670.0
51	167580.5
52	164798.5
53	162774.5
54	161200.0
55	153874.5
56	143269.5
57	130532.0
58	119613.5
59	108014.5
60	92885.0
61	78412.5
62	64466.5
63	49189.0
64	35939.0
65	26890.0
66	19479.5
67	14299.0
68	10417.5
69	7476.0
70	5653.0
71	4268.0
72	3090.5
73	2468.0
74	2013.0
75	1478.5
76	1039.0
77	689.0
78	474.0
79	313.0
80	202.5
81	160.0
82	125.0
83	92.5
84	79.0
85	63.0
86	51.0
87	46.0
88	36.5
89	25.5
90	21.0
91	16.5
92	17.0
93	18.0
94	12.5
95	11.5
96	12.0
97	12.0
98	15.0
99	23.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2934105543065885
2	0.07323795129109549
3	0.08187322926204263
4	0.08802586481634248
5	0.09544680682262519
6	0.12739733531512962
7	0.11673816406974176
8	0.11668419358242332
9	0.12340351925356656
10-11	0.1018153243261987
12-13	0.09143949813923252
14-15	0.10065495884885269
16-17	0.10288124145073749
18-19	0.09462375689101929
20-21	0.09366578074111734
22-23	0.07634125431190462
24-25	0.08768854927060235
26-27	0.10008826873200928
28-29	0.09280225294402261
30-31	0.08820126890012735
32-33	0.09668812803094884
34-35	0.09068391131677464
36-37	0.09107519734983319
38-39	0.0806858785410374
40-41	0.09251890788560091
42-43	0.0823319784042492
44-45	0.0787429409975743
46-47	0.069122701633066
48-49	0.07145692520958764
50-51	0.07319747342560667
52-53	0.08523289209761427
54-55	0.0905624777203082
56-57	0.09842867624696788
58-59	0.06848854840707456
60-61	0.08500351752651097
62-63	0.0908458227787299
64-65	0.08180576615289462
66-67	0.0765436436393487
68-69	0.08404554137660904
70-71	0.08214308169863474
72-73	0.06413043155611217
74-75	0.09149346862655094
76-77	0.09513647652054427
78-79	0.07762305338571708
80-81	0.08991483187248717
82-83	0.09388166269039101
84-85	0.09757864107170276
86-87	0.08602895678556095
88-89	0.06572256093200556
90-91	0.08987435400699835
92-93	0.06623528056153054
94-95	0.09291019391865946
96-97	0.08161686944728015
98-99	0.07621982071543819
100-101	0.09172284319765422
102-103	0.06415741679977138
104-105	0.07169979240252053
106-107	0.08853858444586747
108-109	0.09452930853821205
110-111	0.08771553451426156
112-113	0.07755559027656907
114-115	0.09107519734983319
116-117	0.0847876355772373
118-119	0.07721827473082894
120-121	0.10954659663456232
122-123	0.088349687740253
124-125	0.10965453760919915
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3705729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.22427187874457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.58191396703347	29.644032952757243
2	13.350360778773274	11.54119247962048
3	6.104328962381244	7.915655241217849
4	3.4070127884561154	5.890625882503471
5	2.1156843530834357	4.572445784364213
6	1.3981530723142641	3.6260489115488266
7	0.9497280043411913	2.873591102935081
8	0.7140013393583058	2.468975041136894
9	0.5279694347757025	2.0538984953170853
>10	2.6483009485468787	20.106665490219825
>50	0.14385540767133856	4.2197814584342765
>100	0.05624749284127536	4.009041965578059
>500	0.001691619530157332	0.5010819516189208
>1k	7.518308932841247E-4	0.5769632427477802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6985243659209833E-5
2	0.0	0.0	0.0	2.6985243659209833E-5	2.6985243659209833E-5
3	0.0	0.0	0.0	2.6985243659209833E-5	2.6985243659209833E-5
4	0.0	0.0	0.0	2.6985243659209833E-5	2.6985243659209833E-5
5	0.0	0.0	0.0	2.6985243659209833E-5	2.6985243659209833E-5
6	0.0	0.0	0.0	2.6985243659209833E-5	1.0794097463683933E-4
7	0.0	0.0	0.0	2.6985243659209833E-5	1.0794097463683933E-4
8	0.0	0.0	0.0	2.6985243659209833E-5	1.0794097463683933E-4
9	0.0	0.0	0.0	2.6985243659209833E-5	1.0794097463683933E-4
10-11	0.0	0.0	0.0	1.0794097463683933E-4	1.61911461955259E-4
12-13	0.0	0.0	0.0	1.0794097463683933E-4	5.801827386730115E-4
14-15	0.0	0.0	0.0	1.2143359646644426E-4	6.746310914802459E-4
16-17	0.0	0.0	0.0	1.61911461955259E-4	7.555868224578754E-4
18-19	0.0	0.0	0.0	2.4286719293288851E-4	8.09557309776295E-4
20-21	0.0	0.0	0.0	3.23822923910518E-4	0.0010659171245387886
22-23	0.0	0.0	0.0	6.61138469650641E-4	0.001160365477346023
24-25	0.0	0.0	0.0	0.0012278285864940476	0.001160365477346023
26-27	0.0	0.0	0.0	0.002104849005418367	0.0012278285864940476
28-29	0.0	0.0	0.0	0.005491497084649201	0.0012413212083236525
30-31	0.0	0.0	0.0	0.01682529942151733	0.0012413212083236525
32-33	0.0	0.0	0.0	0.044053410273660054	0.0012413212083236525
34-35	0.0	0.0	0.0	0.08575910434896886	0.0012683064519828622
36-37	0.0	0.0	0.0	0.15565088542632233	0.0012683064519828622
38-39	0.0	0.0	0.0	0.27619396885201264	0.0013492621829604918
40-41	0.0	0.0	0.0	0.4176236308699314	0.0013762474266197015
42-43	0.0	0.0	0.0	0.5454122522181195	0.0013762474266197015
44-45	0.0	0.0	0.0	0.6952208323922229	0.0014437105357677261
46-47	0.0	0.0	0.0	0.8827277979582425	0.0015246662667453557
48-49	0.0	0.0	0.0	1.0865068654507657	0.00161911461955259
50-51	0.0	0.0	0.0	1.305465132501594	0.0017000703505302196
52-53	0.0	0.0	0.0	1.5172318321172433	0.0018889670561446884
54-55	0.0	0.0	0.0	1.752961428102271	0.0018889670561446884
56-57	0.0	0.0	0.0	2.0372779552957057	0.0019429375434631081
58-59	0.0	0.0	0.0	2.3502258260115623	0.001996908030781528
60-61	0.0	0.0	0.0	2.692371730366683	0.002104849005418367
62-63	0.0	0.0	0.0	3.034962891242182	0.002266760467373626
64-65	0.0	0.0	0.0	3.398130300407828	0.002361208820180861
66-67	0.0	0.0	0.0	3.8149983444553017	0.0023747014420104655
68-69	0.0	0.0	0.0	4.287941724826613	0.0023747014420104655
70-71	0.0	0.0	0.0	4.815975480128202	0.0023747014420104655
72-73	0.0	0.0	0.0	5.34878562355747	0.0024826424166473045
74-75	0.0	0.0	0.0	5.88772141729738	0.002644553878602564
76-77	0.0	0.0	0.0	6.469941541866661	0.0026985243659209835
78-79	0.0	0.0	0.0	7.12637108649877	0.002752494853239403
80-81	0.0	0.0	0.0	7.841439565602341	0.0028469432060466374
82-83	0.0	0.0	0.0	8.587541074914004	0.002941391558853872
84-85	0.0	0.0	0.0	9.32089205659669	0.0029683768025130816
86-87	0.0	0.0	0.0	10.09006594923698	0.0031302882644683406
88-89	0.0	0.0	0.0	10.909108572159486	0.0032921997264235997
90-91	0.0	0.0	0.0	11.80338605440387	0.0032921997264235997
92-93	0.0	0.0	0.0	12.731071268298358	0.003400140701060439
94-95	0.0	0.0	0.0	13.636817479097905	0.003413633322890044
96-97	0.0	0.0	0.0	14.556150760079866	0.0036430078939933275
98-99	0.0	0.0	0.0	15.506881372059317	0.0038588898432670065
100-101	0.0	0.0	0.0	16.54010587390497	0.0038588898432670065
102-103	1.3492621829604917E-5	0.0	0.0	17.600706905442898	0.003953338196074241
104-105	2.6985243659209833E-5	0.0	0.0	18.647154176681568	0.004074771792540685
106-107	2.6985243659209833E-5	0.0	0.0	19.672930751277278	0.004074771792540685
108-109	2.6985243659209833E-5	0.0	0.0	20.718136701307625	0.004074771792540685
110-111	2.6985243659209833E-5	0.0	0.0	21.814884466727058	0.004101757036199895
112-113	2.6985243659209833E-5	0.0	0.0	22.941747764070172	0.004101757036199895
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10370	0.0	31.981323	1
GGTATCA	7730	0.0	30.094389	1
GTACATG	16140	0.0	28.493856	1
TCAACGC	11905	0.0	26.950142	4
TACATGG	16735	0.0	26.925634	2
AACGCAG	12170	0.0	26.455769	6
TATCAAC	12535	0.0	26.117655	2
CAACGCA	12300	0.0	26.084316	5
ACATGGG	16820	0.0	26.01136	3
CATGGGG	10980	0.0	25.805145	4
ATCAACG	12965	0.0	24.930386	3
ACGCAGA	13285	0.0	23.965601	7
CGCAGAG	13830	0.0	22.848755	8
GTACTAG	630	0.0	19.882874	1
GTATAGG	570	0.0	19.882874	1
GCAGAGT	14915	0.0	19.86993	9
AGAGTAC	13405	0.0	18.177343	10-11
ATGGGGC	4870	0.0	18.089582	5
GAGTACT	5045	0.0	17.514936	12-13
ATGGGGA	5290	0.0	17.44102	5
>>END_MODULE