FastQCFastQC Report
Mon 15 Aug 2016
SRR2921082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705729
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.23695742457152158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77790.2099182104249933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.1497681023086146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85000.045.2609331
GTATCAA130250.043.503941
TCAACGC164100.034.2096864
CAACGCA167400.033.5001565
TATCAAC169350.033.2890172
ATCAACG174350.032.301053
AACGCAG173800.032.2282036
ACGCAGA190100.029.352257
CGCAGAG195700.028.330718
GTACATG159600.028.1787151
CATGGGG106300.027.3025044
TACATGG163850.026.9141252
ACATGGG163650.026.219433
GTATAGG5950.026.063881
GCAGAGT210150.025.7882219
GAGTACT124250.023.79681212-13
GTATCGC2552.4714609E-823.3429666
AGAGTAC196250.023.0833310-11
GTACTTT130000.022.72229614-15
AGTACTT130600.022.52588512-13
Produced by FastQC (version 0.11.5)