##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3705729
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18097518733831	33.0	31.0	34.0	28.0	34.0
2	31.26829322921347	33.0	31.0	34.0	28.0	34.0
3	31.379723126003007	33.0	31.0	34.0	28.0	34.0
4	34.85239800319991	37.0	35.0	37.0	32.0	37.0
5	34.58163643374893	37.0	35.0	37.0	32.0	37.0
6	34.33261606555687	37.0	35.0	37.0	30.0	37.0
7	34.2821083247048	37.0	35.0	37.0	30.0	37.0
8	34.26510141459346	37.0	35.0	37.0	30.0	37.0
9	35.81575393127776	39.0	37.0	39.0	30.0	39.0
10-11	35.78069213911756	39.0	37.0	39.0	30.0	39.0
12-13	35.718206458162484	39.0	37.0	39.0	30.0	39.0
14-15	36.9781180706954	40.0	37.0	41.0	30.0	41.0
16-17	36.96228636794542	40.0	37.0	41.0	30.0	41.0
18-19	36.979353725002554	40.0	37.0	41.0	30.0	41.0
20-21	36.9803887440231	40.0	37.0	41.0	30.0	41.0
22-23	36.93658035976188	40.0	37.0	41.0	30.0	41.0
24-25	36.891609046425145	40.0	37.0	41.0	30.0	41.0
26-27	36.7765530075189	40.0	37.0	41.0	30.0	41.0
28-29	36.650389977248736	40.0	37.0	41.0	30.0	41.0
30-31	36.5107595563518	40.0	36.5	41.0	30.0	41.0
32-33	36.384407764302246	40.0	36.0	41.0	29.5	41.0
34-35	36.239895712827355	40.0	36.0	41.0	29.0	41.0
36-37	36.06575210977381	40.0	35.5	41.0	28.0	41.0
38-39	35.923636347935854	40.0	35.0	41.0	27.0	41.0
40-41	35.78775552664537	40.0	35.0	41.0	27.0	41.0
42-43	35.63057957017364	40.0	35.0	41.0	26.0	41.0
44-45	35.4491904291976	40.0	35.0	41.0	25.5	41.0
46-47	35.16607892805977	40.0	35.0	41.0	24.0	41.0
48-49	34.898285195706436	39.0	35.0	41.0	23.0	41.0
50-51	34.69027349274596	39.0	35.0	41.0	22.5	41.0
52-53	34.47430937340534	39.0	34.0	41.0	21.5	41.0
54-55	34.25091270840366	39.0	34.0	41.0	20.5	41.0
56-57	33.99204124208759	38.0	34.0	41.0	19.5	41.0
58-59	33.723931917309656	38.0	34.0	40.0	18.5	41.0
60-61	33.4209707455672	37.0	33.0	40.0	17.0	41.0
62-63	33.11835485001737	37.0	33.0	40.0	14.0	41.0
64-65	32.79529641805971	36.0	33.0	39.5	12.5	41.0
66-67	32.449335474882275	36.0	32.5	39.0	9.0	41.0
68-69	32.09374633169344	35.0	32.0	39.0	7.5	41.0
70-71	31.709409808434454	35.0	32.0	38.0	6.5	40.0
72-73	31.33712341080527	35.0	32.0	37.0	2.0	39.5
74-75	31.028734831931857	35.0	31.0	37.0	2.0	39.0
76-77	30.74650790708117	35.0	31.0	36.0	2.0	39.0
78-79	30.460778027751083	35.0	31.0	36.0	2.0	37.5
80-81	30.436183271901427	35.0	31.0	35.5	2.0	37.0
82-83	30.255596806998028	35.0	31.0	35.0	2.0	37.0
84-85	30.074972697679726	35.0	31.0	35.0	2.0	36.0
86-87	29.921998073793304	35.0	31.0	35.0	2.0	36.0
88-89	29.764675722374733	35.0	31.0	35.0	2.0	36.0
90-91	29.62466872240253	35.0	31.0	35.0	2.0	35.5
92-93	29.526612577444276	35.0	31.0	35.0	2.0	35.0
94-95	29.433566108045135	35.0	31.0	35.0	2.0	35.0
96-97	29.3719096836277	35.0	31.0	35.0	2.0	35.0
98-99	29.289827183801084	35.0	31.0	35.0	2.0	35.0
100-101	29.204125558021108	35.0	30.5	35.0	2.0	35.0
102-103	27.892919314931017	32.5	28.0	34.5	2.0	35.0
104-105	28.904570463733315	34.0	30.0	35.0	2.0	35.0
106-107	29.071536531678383	34.5	30.0	35.0	2.0	35.0
108-109	29.07106415498813	35.0	30.0	35.0	2.0	35.0
110-111	28.98541420594976	35.0	30.0	35.0	2.0	35.0
112-113	28.87755310763415	34.0	30.0	35.0	2.0	35.0
114-115	28.78791676347623	34.0	30.0	35.0	2.0	35.0
116-117	28.6774441682055	34.0	30.0	35.0	2.0	35.0
118-119	28.534148071809895	34.0	30.0	35.0	2.0	35.0
120-121	28.66454886474429	35.0	30.5	35.0	2.0	35.0
122-123	28.573699398957668	35.0	31.0	35.0	2.0	35.0
124-125	27.899725263234306	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79713.0
3	27653.0
4	11960.0
5	10705.0
6	12061.0
7	13917.0
8	15139.0
9	16724.0
10	18678.0
11	21270.0
12	22911.0
13	26834.0
14	34041.0
15	31444.0
16	18655.0
17	15807.0
18	15020.0
19	14425.0
20	14190.0
21	14772.0
22	15506.0
23	16633.0
24	21723.0
25	28289.0
26	35472.0
27	41950.0
28	50742.0
29	60481.0
30	72848.0
31	89174.0
32	112533.0
33	146240.0
34	201626.0
35	311552.0
36	620362.0
37	1071027.0
38	373027.0
39	625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10962170181202	17.979436131769653	12.35751373771931	25.55342842869902
2	16.475259784475302	20.308273349161183	37.382353790520185	25.83411307584333
3	19.046751664787145	24.17956089071813	29.5046939482083	27.268993496286427
4	13.34368886764769	16.301438881343223	35.084724768302564	35.27014748270652
5	14.012545724984218	37.237270510303794	33.94196386104794	14.808219903664051
6	33.507231989431055	35.67649124759008	16.392860451054478	14.423416311924392
7	28.58554302316983	31.940356590963187	20.756367290959034	18.71773309490795
8	27.130215306788624	34.219143485051426	19.500220091863728	19.15042111629622
9	26.882635149431938	14.660026686588045	18.67456612703334	39.782772036946675
10-11	25.78774191154395	25.177313529505028	27.24477383357903	21.790170725371997
12-13	26.54235458428073	22.807459534588734	27.325052458286386	23.32513342284415
14-15	23.75072919757702	23.963857979098158	24.90172447552089	27.38368834780393
16-17	23.520727486582494	26.789456432218966	25.698580121320635	23.991235959877905
18-19	23.609910220057824	25.59425382459153	27.695007575566393	23.100828379784257
20-21	24.37042416364147	24.84293921854712	27.580927245340607	23.2057093724708
22-23	24.04617081598574	24.709721765040666	27.404481445446805	23.839625973526793
24-25	23.74184900406558	24.89643059392209	27.62427114076424	23.73744926124809
26-27	23.530281052607144	25.03678953417378	27.643704563035886	23.789224850183192
28-29	23.593818975014166	25.1015845283548	27.63454104738308	23.670055449247954
30-31	23.81529109851335	25.039796318427232	27.506338057156892	23.638574525902527
32-33	23.486352467833026	25.15508896679964	27.441738561960953	23.916820003406382
34-35	23.67086092911861	25.145587252076552	27.635490278482937	23.5480615403219
36-37	24.000372490497107	24.875941095216533	27.34620090059025	23.77748551369611
38-39	23.873742811389928	24.978778637368546	27.32273718195959	23.82474136928194
40-41	23.84787660441336	24.973864301672766	27.41615184806987	23.762107245844
42-43	23.93524610993915	24.876253708474966	27.26788351026092	23.920616671324964
44-45	23.75579724561156	25.049607767290105	27.194976678748173	23.999618308350165
46-47	23.969142046593298	25.077931043325165	26.92128347168171	24.031643438399826
48-49	23.94261904164261	25.27835829536465	26.458255229988875	24.320767433003862
50-51	23.8209126747274	25.467182361268982	26.445452017042697	24.26645294696092
52-53	23.987615032858432	25.46530668096753	26.331698396037716	24.215379890136315
54-55	23.720442900358215	25.577687318220192	26.252053963366578	24.44981581805501
56-57	23.74528141605627	25.520466235835475	26.138495642634936	24.59575670547332
58-59	23.632071881641	25.357443399847025	26.368386932750226	24.64209778576175
60-61	23.639688065988345	25.460378234901093	26.432152517146395	24.46778118196416
62-63	23.47775945191918	25.478219892490095	26.4895292953289	24.55449136026182
64-65	23.45022091451876	25.354713664093055	26.453800003697992	24.741265417690194
66-67	23.442587575033034	25.48574522500497	26.329669902561477	24.74199729740052
68-69	23.659823114518883	25.34581683758903	26.267801638652244	24.72655840923985
70-71	23.383491879686968	25.183261230001197	26.374730835595628	25.058516054716208
72-73	23.484542246558775	25.171759189587352	26.30867727574262	25.035021288111253
74-75	23.327808043244357	25.319624964019038	26.326147217425135	25.026419775311464
76-77	23.3443064750442	25.088620398654832	26.395614413623754	25.171458712677218
78-79	23.298559142910467	25.196720654444082	26.271631647549565	25.233088555095883
80-81	23.193335963055066	25.265598071189526	26.288423075173924	25.25264289058149
82-83	23.081487451204303	25.15382444789523	26.356080841182727	25.408607259717748
84-85	22.948303472592293	25.190291461542337	26.207518329448448	25.653886736416926
86-87	22.907770458776135	25.14258109394598	26.26253003260828	25.68711841466961
88-89	22.894416429167354	25.036971078751165	26.203176152378877	25.865436339702608
90-91	22.726155692297976	25.153146967242684	26.185273788707452	25.935423551751885
92-93	22.740636280827236	25.087426200195672	26.248790526635403	25.92314699234169
94-95	22.54904646400583	24.936593988995455	26.406479929020353	26.107879617978362
96-97	22.57985632346398	24.826679769965313	26.356820978161604	26.236642928409104
98-99	22.513779757623098	24.96687486892327	26.216074912865185	26.30327046058845
100-101	22.455681041976835	24.967348471873393	26.202620193186917	26.374350292962855
102-103	22.248027000594202	24.895791595877466	26.28914166445454	26.56703973907379
104-105	22.138227848716205	25.087818944601903	26.100488002227134	26.673465204454754
106-107	22.030919722541125	25.084295292702542	26.180791995857472	26.703992988898868
108-109	21.921781754023755	25.112098425582012	26.244923708029294	26.721196112364932
110-111	21.956001676733276	25.25031560212188	26.120559605681564	26.673123115463277
112-113	21.835977674258423	25.39690079797833	26.00917315432003	26.757948373443224
114-115	21.76152364563655	25.54982924758688	25.944974635099115	26.74367247167745
116-117	21.573364452813262	25.467325220785746	26.041436983727856	26.917873342673136
118-119	21.520424845861676	25.503030950727013	26.011985627610017	26.964558575801295
120-121	21.499398746543065	25.556118968305196	26.013859593824684	26.930622691327056
122-123	21.397352454420883	25.671058552708363	25.920929859135356	27.010659133735395
124-125	21.242977873936013	25.750455885835255	25.889512028437288	27.117054211791437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	193.0
2	315.5
3	592.0
4	458.5
5	265.0
6	477.0
7	1074.5
8	1705.5
9	1853.5
10	1502.0
11	1184.5
12	1258.5
13	1390.5
14	1573.0
15	1785.5
16	2017.5
17	2267.0
18	2594.5
19	2974.5
20	3426.5
21	4037.5
22	4711.5
23	5522.0
24	6622.0
25	8243.0
26	10419.5
27	13428.5
28	16778.0
29	20328.5
30	24580.5
31	29688.5
32	35593.0
33	40268.5
34	43704.0
35	48419.5
36	54406.0
37	60857.5
38	67551.5
39	74281.5
40	81837.5
41	89601.0
42	96206.5
43	104799.0
44	114347.5
45	123618.0
46	133896.5
47	143434.5
48	153178.0
49	160687.5
50	166300.0
51	168850.5
52	166197.5
53	164245.0
54	162305.0
55	155257.5
56	144529.0
57	132275.5
58	121552.0
59	109355.5
60	93712.0
61	79148.0
62	64969.5
63	48944.0
64	35096.0
65	25831.5
66	18579.0
67	13488.0
68	9642.5
69	6806.0
70	5058.0
71	3709.5
72	2628.0
73	2123.5
74	1658.5
75	1210.0
76	837.0
77	464.5
78	267.0
79	163.5
80	108.5
81	75.5
82	51.5
83	36.0
84	23.5
85	18.0
86	17.5
87	14.5
88	8.0
89	9.0
90	14.0
91	11.5
92	5.0
93	7.0
94	11.0
95	13.0
96	12.5
97	9.5
98	6.5
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1733801905104232
2	0.012035418672007586
3	0.0
4	0.0038319045996077963
5	0.14423612735847657
6	0.0757205937077428
7	0.00828446980337742
8	0.012359241595918103
9	0.013411666098627286
10-11	0.005842305252218929
12-13	0.010902038438320773
14-15	0.0123457489740885
16-17	0.015880815893444988
18-19	0.019577794274756734
20-21	0.02357161033631979
22-23	0.026027267509307887
24-25	0.026094730618455908
26-27	0.023504147227171764
28-29	0.02184455474213036
30-31	0.025001828250257912
32-33	0.024826424166473047
34-35	0.024448630755244113
36-37	0.02527168068685001
38-39	0.01986113933317844
40-41	0.027686859994349288
42-43	0.02349065460534216
44-45	0.03151876459395708
46-47	0.027295573961290745
48-49	0.0189436410487653
50-51	0.015233170045623952
52-53	0.015975264246252223
54-55	0.018808714830469253
56-57	0.015260155289283161
58-59	0.014221223408403583
60-61	0.017553901000315997
62-63	0.015705411809660123
64-65	0.027403514935927586
66-67	0.020981026945035643
68-69	0.019780183602200806
70-71	0.0263510904332184
72-73	0.03228784403824456
74-75	0.017391989538360738
76-77	0.008581307483628727
78-79	0.014531553710484495
80-81	0.017472945269338368
82-83	0.016528461741266025
84-85	0.015044273340009484
86-87	0.010294870455988552
88-89	0.021750106389323128
90-91	0.019146030376209378
92-93	0.01629908717016274
94-95	0.011913985075541141
96-97	0.02184455474213036
98-99	0.022613634186417843
100-101	0.029103585286457803
102-103	0.02087308597039881
104-105	0.014045819324618718
106-107	0.02017146963525935
108-109	0.01056472289258065
110-111	0.024448630755244113
112-113	0.018309487822773873
114-115	0.01377596688802662
116-117	0.011873507210052328
118-119	0.007852705904830061
120-121	0.003696978381311747
122-123	0.005977231470514978
124-125	8.500351752651098E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3705729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.78169774026657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.38259688358417	27.071950013202915
2	14.256269592062978	11.627897548145317
3	6.451666866384807	7.89329784197394
4	3.5790039554321798	5.838314300866147
5	2.2749612405973023	4.638839084243053
6	1.4863036313183997	3.6368391265612554
7	1.0609289265108037	3.028653796340836
8	0.7752584026521442	2.52930830780493
9	0.5949893365451242	2.18382077534985
>10	2.906455068922638	20.726495877430036
>50	0.1643959591509209	4.554814354253265
>100	0.06359328906618963	4.3832656564887555
>500	0.0025832789498392407	0.7428560982344667
>1k	7.948550580139808E-4	0.5463909181292845
>5k	1.9871376450349504E-4	0.5972563009758479
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8781	0.23695742457152158	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7779	0.2099182104249933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5550	0.1497681023086146	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6985243659209836E-4
2	0.0	0.0	0.0	0.0	2.6985243659209836E-4
3	0.0	0.0	0.0	0.0	2.6985243659209836E-4
4	0.0	0.0	0.0	0.0	2.6985243659209836E-4
5	0.0	0.0	0.0	0.0	2.968376802513082E-4
6	0.0	0.0	0.0	0.0	3.508081675697278E-4
7	0.0	0.0	0.0	0.0	3.508081675697278E-4
8	0.0	0.0	0.0	0.0	3.508081675697278E-4
9	0.0	0.0	0.0	0.0	3.508081675697278E-4
10-11	0.0	0.0	0.0	2.6985243659209833E-5	3.508081675697278E-4
12-13	0.0	0.0	0.0	2.6985243659209833E-5	6.47645847821036E-4
14-15	0.0	0.0	0.0	4.047786548881475E-5	8.230499316059E-4
16-17	0.0	0.0	0.0	8.09557309776295E-5	9.444835280723442E-4
18-19	0.0	0.0	0.0	1.6191146195525903E-4	0.0010524245027091835
20-21	0.0	0.0	0.0	2.293745711032836E-4	0.0012143359646644425
22-23	0.0	0.0	0.0	5.127196295249868E-4	0.0013087843174716769
24-25	0.0	0.0	0.0	0.0010524245027091835	0.0013492621829604918
26-27	0.0	0.0	0.0	0.001956430165292713	0.0014302179139381213
28-29	0.0	0.0	0.0	0.0053835561100123615	0.0014302179139381213
30-31	0.0	0.0	0.0	0.016460998632117998	0.001457203157597331
32-33	0.0	0.0	0.0	0.04329782345120218	0.0015516515104045654
34-35	0.0	0.0	0.0	0.08454476838430441	0.0015921293758933803
36-37	0.0	0.0	0.0	0.15358651428639278	0.0015921293758933803
38-39	0.0	0.0	0.0	0.2726589019326562	0.0016595924850414047
40-41	0.0	0.0	0.0	0.4123750009782151	0.0016730851068710098
42-43	0.0	0.0	0.0	0.5392056461765014	0.0017000703505302196
44-45	0.0	0.0	0.0	0.6874895600838593	0.0017270555941894293
46-47	0.0	0.0	0.0	0.8731615290810526	0.001781026081507849
48-49	0.0	0.0	0.0	1.0754024376850007	0.0018484891906558737
50-51	0.0	0.0	0.0	1.2935646400478826	0.0019159522998038981
52-53	0.0	0.0	0.0	1.5030241013306693	0.002104849005418367
54-55	0.0	0.0	0.0	1.7376473023256693	0.002104849005418367
56-57	0.0	0.0	0.0	2.020601074714314	0.0021723121145663916
58-59	0.0	0.0	0.0	2.331214721853649	0.002253267845544021
60-61	0.0	0.0	0.0	2.671593092749092	0.0022937457110328357
62-63	0.0	0.0	0.0	3.0131183365000513	0.0023747014420104655
64-65	0.0	0.0	0.0	3.374801557264441	0.0024151793074992803
66-67	0.0	0.0	0.0	3.790225890776147	0.0024691497948177
68-69	0.0	0.0	0.0	4.2627779851144005	0.0025635981476249342
70-71	0.0	0.0	0.0	4.7877893931261575	0.002644553878602564
72-73	0.0	0.0	0.0	5.3177391007275485	0.0028064653405578226
74-75	0.0	0.0	0.0	5.852262807129177	0.002914406315194662
76-77	0.0	0.0	0.0	6.430097829603838	0.002927898937024267
78-79	0.0	0.0	0.0	7.083437563836966	0.0029683768025130816
80-81	0.0	0.0	0.0	7.795767040709129	0.0029953620461722914
82-83	0.0	0.0	0.0	8.539048592058405	0.0031437808862979457
84-85	0.0	0.0	0.0	9.27073998125605	0.0031707661299571555
86-87	0.0	0.0	0.0	10.037309797883225	0.0032921997264236
88-89	0.0	0.0	0.0	10.852790368642715	0.003400140701060439
90-91	0.0	0.0	0.0	11.74194065459185	0.003413633322890044
92-93	0.0	0.0	0.0	12.666657491683821	0.003548559541186093
94-95	0.0	0.0	0.0	13.56969168549562	0.0035755447848453033
96-97	0.0	0.0	0.0	14.486542324060935	0.004034293927051871
98-99	0.0	0.0	0.0	15.436260989403165	0.004317638985473574
100-101	0.0	0.0	0.0	16.469552954357965	0.004317638985473574
102-103	0.0	0.0	0.0	17.530734168634567	0.004385102094621599
104-105	0.0	0.0	0.0	18.576412360428947	0.004452565203769623
106-107	0.0	0.0	0.0	19.599571366389718	0.004452565203769623
108-109	0.0	0.0	0.0	20.643967759110286	0.004452565203769623
110-111	0.0	0.0	0.0	21.741565559704988	0.004479550447428832
112-113	0.0	0.0	0.0	22.868914591433963	0.004479550447428832
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8500	0.0	45.260933	1
GTATCAA	13025	0.0	43.50394	1
TCAACGC	16410	0.0	34.209686	4
CAACGCA	16740	0.0	33.500156	5
TATCAAC	16935	0.0	33.289017	2
ATCAACG	17435	0.0	32.30105	3
AACGCAG	17380	0.0	32.228203	6
ACGCAGA	19010	0.0	29.35225	7
CGCAGAG	19570	0.0	28.33071	8
GTACATG	15960	0.0	28.178715	1
CATGGGG	10630	0.0	27.302504	4
TACATGG	16385	0.0	26.914125	2
ACATGGG	16365	0.0	26.21943	3
GTATAGG	595	0.0	26.06388	1
GCAGAGT	21015	0.0	25.788221	9
GAGTACT	12425	0.0	23.796812	12-13
GTATCGC	255	2.4714609E-8	23.342966	6
AGAGTAC	19625	0.0	23.08333	10-11
GTACTTT	13000	0.0	22.722296	14-15
AGTACTT	13060	0.0	22.525885	12-13
>>END_MODULE