FastQCFastQC Report
Mon 15 Aug 2016
SRR2921081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815643
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41770.23005623902936867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.1064085836257458No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19230.10591289146599854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18590.10238796944112911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA1254.8838523E-528.5602517
GGTATCA49450.027.371041
GTATCAA65350.025.912211
TACATGG82600.025.7227122
CATGGGG45000.025.5254484
GTACATG87050.025.000911
ACATGGG84750.024.016793
AACGCAG70500.022.6217586
TCAACGC74700.021.6708954
TATCAAC76450.021.6419242
GCGTCTA1653.2160777E-421.6365539
GAGTACT27150.021.47598612-13
ATCAACG78300.020.9025613
CAACGCA77300.020.8649985
ACGGCGT1450.003868027620.5174228
ACGCAGA78700.020.2619327
CGCAGAG80350.019.6977468
GTGTAGC15900.019.5001491
GCACCGT2801.564993E-619.1278066
CCGTGTA2801.5670594E-619.1251689
Produced by FastQC (version 0.11.5)