##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921081_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1815643
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.753953282666252	31.0	30.0	34.0	25.0	34.0
2	29.77285898163901	31.0	30.0	34.0	25.0	34.0
3	29.893211936487514	31.0	31.0	34.0	25.0	34.0
4	33.254117687232565	37.0	35.0	37.0	26.0	37.0
5	33.38011216962806	37.0	35.0	37.0	27.0	37.0
6	33.4050085837359	37.0	35.0	37.0	27.0	37.0
7	33.32536957981277	37.0	35.0	37.0	27.0	37.0
8	33.27267309707911	37.0	35.0	37.0	26.0	37.0
9	34.671204085825245	39.0	35.0	39.0	26.0	39.0
10-11	34.67288310532412	39.0	35.0	39.0	26.5	39.0
12-13	34.602340052532355	39.0	35.0	39.0	25.5	39.0
14-15	35.80253689739668	40.0	36.0	41.0	26.0	41.0
16-17	35.78009773947852	40.0	36.0	41.0	26.0	41.0
18-19	35.81907346323038	40.0	36.0	41.0	26.0	41.0
20-21	35.88578371408917	40.0	36.0	41.0	26.5	41.0
22-23	35.821202461056494	40.0	36.0	41.0	26.5	41.0
24-25	35.8087743570735	40.0	36.0	41.0	27.0	41.0
26-27	35.75054677599066	40.0	36.0	41.0	27.0	41.0
28-29	35.6059112391588	40.0	36.0	41.0	26.0	41.0
30-31	35.47819009574019	40.0	35.0	41.0	25.0	41.0
32-33	35.339856733950455	40.0	35.0	41.0	25.0	41.0
34-35	35.20875083923437	40.0	35.0	41.0	24.5	41.0
36-37	35.05568688337961	40.0	35.0	41.0	24.0	41.0
38-39	34.89709733686633	40.0	35.0	41.0	23.0	41.0
40-41	34.764926530160395	40.0	35.0	41.0	22.5	41.0
42-43	34.55888657627078	39.5	34.5	41.0	20.5	41.0
44-45	34.35262163321754	39.0	34.0	41.0	19.0	41.0
46-47	34.15565312123584	39.0	34.0	41.0	17.0	41.0
48-49	33.91339183969536	39.0	33.0	41.0	14.0	41.0
50-51	33.72935869000679	39.0	33.0	41.0	11.0	41.0
52-53	33.55133057544903	38.0	33.0	40.0	8.5	41.0
54-55	33.32719593003691	38.0	33.0	40.0	7.5	41.0
56-57	33.08866721045932	38.0	33.0	40.0	6.0	41.0
58-59	32.82339149271085	37.5	32.0	40.0	2.0	41.0
60-61	32.53201124890741	37.0	32.0	40.0	2.0	41.0
62-63	32.251613064903175	36.5	31.5	40.0	2.0	41.0
64-65	31.935398643896406	36.0	31.0	39.5	2.0	41.0
66-67	31.59586438523432	35.5	31.0	39.0	2.0	41.0
68-69	31.229752765273787	35.0	31.0	39.0	2.0	40.0
70-71	30.867332950365242	35.0	30.5	38.0	2.0	40.0
72-73	30.50626169351574	35.0	30.0	37.0	2.0	39.5
74-75	30.45470502736496	35.0	30.0	37.0	2.0	39.0
76-77	30.481736773143176	35.0	31.0	37.0	2.0	39.0
78-79	30.287586271089637	35.0	31.0	36.0	2.0	39.0
80-81	30.058453671784598	35.0	31.0	36.0	2.0	37.5
82-83	29.79734755125319	35.0	31.0	35.5	2.0	37.0
84-85	29.528836065239695	35.0	30.5	35.0	2.0	37.0
86-87	29.3665045936894	35.0	30.0	35.0	2.0	36.0
88-89	29.175878187507124	35.0	30.0	35.0	2.0	36.0
90-91	29.020255909339006	35.0	30.0	35.0	2.0	36.0
92-93	28.89122613861866	35.0	30.0	35.0	2.0	35.5
94-95	28.76784340313597	35.0	30.0	35.0	2.0	35.0
96-97	28.659074223291693	35.0	30.0	35.0	2.0	35.0
98-99	28.551494704630812	35.0	29.0	35.0	2.0	35.0
100-101	28.40653421404979	34.5	29.0	35.0	2.0	35.0
102-103	27.13103181627666	32.5	26.0	34.5	2.0	35.0
104-105	28.055800617191814	34.0	29.0	35.0	2.0	35.0
106-107	28.17448859715263	34.0	29.0	35.0	2.0	35.0
108-109	28.09795648153299	34.0	29.0	35.0	2.0	35.0
110-111	27.991904520877725	34.0	29.0	35.0	2.0	35.0
112-113	27.859676709573414	34.0	29.0	35.0	2.0	35.0
114-115	27.69950920968494	34.0	29.0	35.0	2.0	35.0
116-117	27.53664789829278	34.0	28.0	35.0	2.0	35.0
118-119	27.30723247907215	34.0	27.0	35.0	2.0	35.0
120-121	27.073201615075213	34.0	27.0	35.0	2.0	35.0
122-123	26.743490047327587	34.0	25.5	35.0	2.0	35.0
124-125	25.477936466585113	33.0	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69326.0
3	10204.0
4	7293.0
5	8085.0
6	12911.0
7	12398.0
8	9844.0
9	10190.0
10	10740.0
11	11279.0
12	11950.0
13	11799.0
14	11863.0
15	10689.0
16	7852.0
17	7101.0
18	6751.0
19	6648.0
20	6976.0
21	7444.0
22	8980.0
23	11488.0
24	15868.0
25	19705.0
26	21347.0
27	23121.0
28	26736.0
29	31632.0
30	37376.0
31	45757.0
32	57115.0
33	73813.0
34	102356.0
35	154522.0
36	286412.0
37	473061.0
38	174760.0
39	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31136401547916	18.786600340028965	12.189397680335212	24.712637964156663
2	16.043071652340313	21.91551677384792	37.67937335893631	24.36203821487545
3	19.193972015290445	25.35286806066569	29.2029291066886	26.25023081735526
4	12.61287184109056	17.434616879304784	36.042147272912686	33.91036400669197
5	13.305831868769172	39.10893490719772	33.55689528568373	14.028337938349383
6	31.91136772852231	37.11812764877973	17.0452233040227	13.925281318675259
7	27.255333027856306	33.95487431605403	20.60723759992104	18.182555056168624
8	25.718648127643995	35.776137772229404	19.789594297133128	18.715619802993473
9	25.977783635282815	16.882187314923367	18.866925695832997	38.27310335396082
10-11	24.41635289716823	27.266783050134478	27.414095521000707	20.902768531696577
12-13	25.054870786636087	25.13017255184507	27.025505854491744	22.789450807027098
14-15	22.83036322698875	26.488710214504824	24.684263104156237	25.996663454350188
16-17	22.660087930275466	28.760874473177793	25.704072088601787	22.874965507944957
18-19	22.65188969033291	27.914444074471817	27.084624256042623	22.349041979152656
20-21	23.917281732911533	27.1352884800116	26.707082746106664	22.240347040970207
22-23	23.33002442273657	27.065363753263664	26.31862659449163	23.28598522950813
24-25	22.93419232426154	27.106172789145273	26.707648258760088	23.251986627833105
26-27	22.82591587064393	27.275373560770767	26.6843141000567	23.214396468528605
28-29	22.888482783713158	27.549917860174865	26.65004575574152	22.911553600370457
30-31	23.222483512267402	27.485728271582886	26.393574469867854	22.898213746281854
32-33	22.66818311624876	27.65843608694262	26.461282572333655	23.212098224474968
34-35	22.96329556301425	27.676192350511208	26.574091760679014	22.786420325795532
36-37	23.062087617371766	27.482592346972247	26.423855334148104	23.031464701507883
38-39	22.86858196133509	27.524896607491	26.48799500386671	23.1185264273072
40-41	23.04357269769901	27.52736145344129	26.5031089491096	22.925956899750105
42-43	22.81209743965419	27.58403434299671	26.416860832700813	23.18700738464829
44-45	22.869842445729102	27.660106314958615	26.427022563817637	23.043028675494647
46-47	22.7331123608634	27.58310718009881	26.32382313971971	23.359957319318077
48-49	22.67641990628711	27.557696976611464	26.468035901137117	23.297847215964314
50-51	22.64059480184984	27.743171885200972	26.26451750340417	23.351715809545016
52-53	22.775590909266302	27.576785543993726	26.035645007389295	23.611978539350677
54-55	22.742318570298867	27.511333369900743	26.131951930579987	23.6143961292204
56-57	22.7675384166703	27.592081201371	26.001330862728732	23.639049519229975
58-59	22.844846115944616	27.259099060966108	26.228851345169634	23.667203477919642
60-61	22.887932733345885	27.317720944372493	26.22797557016769	23.566370752113937
62-63	22.736212035222703	27.334556964014066	26.319231995801545	23.609999004961686
64-65	22.7821548785358	27.211134087559834	26.270073501668662	23.7366375322357
66-67	22.846737355573467	27.48189027373005	26.105702651867468	23.565669718829017
68-69	23.123209200643394	27.299940357217274	26.07938553601884	23.497464906120495
70-71	22.818977104761473	27.09176643995867	26.288913099738746	23.800343355541106
72-73	22.94793758340863	27.088450141927282	26.378276376416345	23.585335898247738
74-75	22.981279317391618	27.189918538376602	26.261326594571173	23.567475549660607
76-77	22.695614325970997	26.957879854644247	26.59603411334011	23.750471706044646
78-79	22.93559271398795	27.053507426097983	26.46519058770226	23.545709272211806
80-81	22.86690736815006	26.93137030882245	26.469629193107952	23.73209312991954
82-83	22.99598523094225	26.78987997039573	26.466658672962396	23.74747612569962
84-85	23.019996234563017	26.79530163879205	26.336171928860253	23.848530197784676
86-87	22.812838498959525	26.634676074583464	26.546421730082827	24.006063696374184
88-89	22.977872792115352	26.615643306036002	26.529171440806454	23.877312461042195
90-91	23.041896361631753	26.686797133406838	26.40887541345094	23.862431091510476
92-93	23.073989373677463	26.742829051013345	26.32532110855234	23.85786046675685
94-95	22.802598088479886	26.83402344828879	26.419375627429435	23.94400283580189
96-97	22.77070761496728	26.84838554535645	26.3599494531208	24.020957386555466
98-99	22.65567125546776	27.09739774410893	26.186150698461972	24.060780301961337
100-101	22.710195621194455	27.287047986041628	26.15689938890772	23.845857003856192
102-103	22.525963876537386	27.542156164643274	26.105076816920224	23.826803141899116
104-105	22.493950261101247	27.725675722971697	25.90010276495321	23.88027125097384
106-107	22.427261509066486	27.816575988463164	25.949778268157026	23.806384234313324
108-109	22.401431172378814	27.943089397278513	25.89782498735458	23.7576544429881
110-111	22.4543763178567	28.324389134659118	25.61196786152341	23.60926668596078
112-113	22.54686072033716	28.47581807137668	25.540925341444137	23.436395866842023
114-115	22.594950839735056	28.542970784993095	25.45330257199009	23.408775803281763
116-117	22.632214185367904	28.509098446121396	25.510542985165706	23.348144383344994
118-119	22.72585587787926	28.458451073183866	25.379360485546144	23.43633256339073
120-121	22.794057238639528	28.679971438575013	25.325851122193825	23.200120200591627
122-123	23.075351963794937	28.720333869808744	25.11876374896258	23.085550417433733
124-125	23.160251451370208	28.903977711011773	24.8653943387602	23.07037649885782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3429.0
1	2330.5
2	1069.5
3	845.5
4	670.0
5	496.5
6	425.5
7	450.0
8	540.5
9	596.0
10	570.5
11	534.5
12	578.0
13	671.0
14	768.5
15	906.0
16	1085.5
17	1275.5
18	1529.5
19	1809.5
20	2238.5
21	2757.0
22	3317.5
23	4119.0
24	5201.5
25	6385.0
26	8036.5
27	9886.0
28	11667.0
29	13949.0
30	16035.5
31	18377.5
32	21889.5
33	25103.5
34	27602.0
35	31168.5
36	36349.5
37	40635.0
38	42800.5
39	45122.0
40	49310.5
41	53162.0
42	55310.0
43	58807.0
44	63311.0
45	66825.5
46	69590.0
47	72504.0
48	75187.5
49	76825.0
50	79301.5
51	78507.0
52	73283.0
53	69977.5
54	66901.0
55	61967.5
56	56015.5
57	50202.0
58	45777.0
59	41011.5
60	35029.5
61	28952.0
62	23581.0
63	17753.5
64	12649.0
65	9523.5
66	7249.0
67	5664.0
68	4397.0
69	3311.0
70	2621.5
71	2126.0
72	1687.0
73	1431.5
74	1150.0
75	818.0
76	605.0
77	431.5
78	307.0
79	222.0
80	162.0
81	124.5
82	90.5
83	78.0
84	62.0
85	50.5
86	48.0
87	38.5
88	26.0
89	18.5
90	17.5
91	15.5
92	10.5
93	11.0
94	9.0
95	6.0
96	7.0
97	8.5
98	15.0
99	17.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28689560668038816
2	0.07000274833764127
3	0.07920059174628492
4	0.08415751334375755
5	0.09247412624618385
6	0.12711750052185367
7	0.11489042724808786
8	0.11560642703438946
9	0.12144457913807946
10-11	0.09977181637579634
12-13	0.08859120432816363
14-15	0.09864273978970536
16-17	0.10021243162890502
18-19	0.09335535675240121
20-21	0.09244658779286456
22-23	0.07435382396208946
24-25	0.08682874331572892
26-27	0.0981195091766388
28-29	0.09159289573996651
30-31	0.08630551270266236
32-33	0.09677012496399348
34-35	0.09101458822026136
36-37	0.09043628070055622
38-39	0.07931074555956209
40-41	0.09285966459265395
42-43	0.08118336038527398
44-45	0.07771351526704313
46-47	0.06824028732520655
48-49	0.07003028679096056
50-51	0.07294936284280555
52-53	0.08437782097031189
54-55	0.09040874224723693
56-57	0.09814704762995809
58-59	0.06746921063226637
60-61	0.08534166683648714
62-63	0.09046381915387551
64-65	0.08077028358548459
66-67	0.07465674694860168
68-69	0.08311105211762444
70-71	0.08198197553153345
72-73	0.0637515194341619
74-75	0.09142766502005074
76-77	0.0963019712575655
78-79	0.07708013084069941
80-81	0.09029858843395976
82-83	0.0944844333384922
84-85	0.09784412464344588
86-87	0.08636058960930094
88-89	0.06499074983353005
90-91	0.0904913576071948
92-93	0.06534874972668085
94-95	0.09330027984576264
96-97	0.0818167448116177
98-99	0.07551043900149974
100-101	0.09241904933954527
102-103	0.06603721105966316
104-105	0.0720681323365882
106-107	0.08963766555429674
108-109	0.09613674053764974
110-111	0.08911443494123018
112-113	0.07914551483964634
114-115	0.09241904933954527
116-117	0.08644320496925882
118-119	0.07922813019960422
120-121	0.11059442853027825
122-123	0.09046381915387551
124-125	0.1108147361568326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1815643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.08671145952821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40772708596344	35.968992381618236
2	14.120993378732615	14.42790228522443
3	5.949902662508603	9.11882881595567
4	3.1241391958089046	6.384079906227681
5	1.8605149182349148	4.752379439700741
6	1.1237917806235658	3.4446495862383317
7	0.7483124967378462	2.6760177221683845
8	0.5457744681261787	2.2305458220111656
9	0.38273359793535655	1.7597340795231577
>10	1.648827300256724	14.084274644745747
>50	0.06294330601585729	2.1604102170377626
>100	0.022609424350529917	1.9586825258911515
>500	0.001081490440939289	0.3526594102242792
>1k	6.488942645635733E-4	0.6808431634331493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4177	0.23005623902936867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1932	0.1064085836257458	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1923	0.10591289146599854	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1859	0.10238796944112911	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.507690663858479E-5	0.0
9	0.0	0.0	0.0	5.507690663858479E-5	0.0
10-11	0.0	0.0	0.0	1.1015381327716958E-4	0.0
12-13	0.0	0.0	0.0	1.1015381327716958E-4	0.0
14-15	0.0	0.0	0.0	1.1015381327716958E-4	0.0
16-17	0.0	0.0	0.0	1.9276917323504677E-4	0.0
18-19	0.0	0.0	0.0	2.7538453319292394E-4	0.0
20-21	0.0	0.0	0.0	3.304614398315087E-4	0.0
22-23	0.0	0.0	0.0	7.986151462594795E-4	0.0
24-25	0.0	0.0	0.0	0.0016798456524768361	0.0
26-27	0.0	0.0	0.0	0.00333215285163438	0.0
28-29	0.0	0.0	0.0	0.007793382289359748	0.0
30-31	0.0	0.0	0.0	0.022443839455223304	0.0
32-33	0.0	0.0	0.0	0.057775675063875445	0.0
34-35	0.0	0.0	0.0	0.11646011908728754	0.0
36-37	0.0	0.0	0.0	0.21493762815707712	0.0
38-39	0.0	0.0	0.0	0.37614773388821476	0.0
40-41	0.0	0.0	0.0	0.5693850608296895	0.0
42-43	0.0	0.0	0.0	0.7424367014881229	0.0
44-45	0.0	0.0	0.0	0.9455327947179044	0.0
46-47	0.0	0.0	0.0	1.1974821041361103	0.0
48-49	0.0	0.0	0.0	1.4684604847979474	0.0
50-51	0.0	0.0	0.0	1.7566503987843425	0.0
52-53	0.0	0.0	0.0	2.0386716992272156	0.0
54-55	0.0	0.0	0.0	2.350902682961353	0.0
56-57	0.0	0.0	0.0	2.729804262181497	0.0
58-59	0.0	0.0	0.0	3.1467915223422223	0.0
60-61	0.0	0.0	0.0	3.587764775344052	0.0
62-63	0.0	0.0	0.0	4.026121875280548	0.0
64-65	0.0	0.0	0.0	4.503886501916952	0.0
66-67	0.0	0.0	0.0	5.048376800946	0.0
68-69	0.0	0.0	0.0	5.686002149100897	0.0
70-71	0.0	0.0	0.0	6.380879941706603	0.0
72-73	0.0	0.0	0.0	7.06862527490261	0.0
74-75	0.0	0.0	0.0	7.755792300578913	0.0
76-77	0.0	0.0	0.0	8.518469765256716	0.0
78-79	0.0	0.0	0.0	9.39008935126564	0.0
80-81	0.0	0.0	0.0	10.325019841455617	0.0
82-83	0.0	0.0	0.0	11.295998167040548	0.0
84-85	0.0	0.0	0.0	12.244422499356977	0.0
86-87	0.0	0.0	0.0	13.211462826117248	0.0
88-89	0.0	0.0	0.0	14.272739740136139	0.0
90-91	0.0	0.0	0.0	15.39237063673861	0.0
92-93	0.0	0.0	0.0	16.540366140259952	0.0
94-95	0.0	0.0	0.0	17.6758041090677	0.0
96-97	0.0	0.0	0.0	18.826360688747734	0.0
98-99	0.0	0.0	0.0	20.04730555511188	0.0
100-101	0.0	0.0	0.0	21.33863870816014	0.0
102-103	0.0	0.0	0.0	22.634405552192803	0.0
104-105	0.0	0.0	0.0	23.909022864076256	0.0
106-107	0.0	0.0	0.0	25.148473571070966	0.0
108-109	0.0	0.0	0.0	26.416757038690974	0.0
110-111	0.0	0.0	0.0	27.740805874282554	0.0
112-113	0.0	0.0	0.0	29.100792391455812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	125	4.8838523E-5	28.560251	7
GGTATCA	4945	0.0	27.37104	1
GTATCAA	6535	0.0	25.91221	1
TACATGG	8260	0.0	25.722712	2
CATGGGG	4500	0.0	25.525448	4
GTACATG	8705	0.0	25.00091	1
ACATGGG	8475	0.0	24.01679	3
AACGCAG	7050	0.0	22.621758	6
TCAACGC	7470	0.0	21.670895	4
TATCAAC	7645	0.0	21.641924	2
GCGTCTA	165	3.2160777E-4	21.636553	9
GAGTACT	2715	0.0	21.475986	12-13
ATCAACG	7830	0.0	20.902561	3
CAACGCA	7730	0.0	20.864998	5
ACGGCGT	145	0.0038680276	20.517422	8
ACGCAGA	7870	0.0	20.261932	7
CGCAGAG	8035	0.0	19.697746	8
GTGTAGC	1590	0.0	19.500149	1
GCACCGT	280	1.564993E-6	19.127806	6
CCGTGTA	280	1.5670594E-6	19.125168	9
>>END_MODULE