FastQCFastQC Report
Mon 15 Aug 2016
SRR2921081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815643
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69720.3839961930842131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.3381722067609106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.23104762334886317No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT26890.1481018019511545No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18980.10453596880003392No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18350.10106612368180308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.043.8180961
GTATCAA92700.037.0670551
ACCGCGC1751.6760168E-830.593428
TCAACGC112850.029.9949744
TATCAAC115250.029.6806032
CAACGCA113850.029.679175
ATCAACG114850.029.4726453
AACGCAG115900.029.1228776
CATGGGG45950.027.624594
ACGCAGA129150.025.8860327
GAGTACT81150.025.54758312-13
CGCAGAG131950.025.3367258
TACATGG88650.025.2089212
GTACATG91600.025.1383741
ACATGGG88000.024.9888023
AGTACTT84450.024.02095412-13
TTAGCGC1250.00161896223.8375574
GCGCCGT1501.6813944E-423.8119346
GCAGAGT139000.023.7092919
GTACTTT89550.022.98571414-15
Produced by FastQC (version 0.11.5)