##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921081_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1815643 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27144543283013 31.0 31.0 34.0 28.0 34.0 2 31.37012892953075 33.0 31.0 34.0 28.0 34.0 3 31.465182858083885 34.0 31.0 34.0 28.0 34.0 4 34.94679075126553 37.0 35.0 37.0 32.0 37.0 5 34.713158919457186 37.0 35.0 37.0 32.0 37.0 6 34.445273107103105 37.0 35.0 37.0 30.0 37.0 7 34.38787360731157 37.0 35.0 37.0 30.0 37.0 8 34.379939778910284 37.0 35.0 37.0 30.0 37.0 9 35.93784516008929 39.0 37.0 39.0 30.0 39.0 10-11 35.916700309477136 39.0 37.0 39.0 30.0 39.0 12-13 35.8558609814815 39.0 37.0 39.0 30.0 39.0 14-15 37.102231826410815 40.0 37.0 41.0 31.0 41.0 16-17 37.09113327895407 40.0 37.0 41.0 31.0 41.0 18-19 37.13641448236244 40.0 37.5 41.0 31.0 41.0 20-21 37.14952719229496 40.0 37.5 41.0 31.0 41.0 22-23 37.09742223553859 40.0 37.0 41.0 30.0 41.0 24-25 37.04544533259016 40.0 37.0 41.0 30.0 41.0 26-27 36.91450714705479 40.0 37.0 41.0 30.0 41.0 28-29 36.77041604544506 40.0 37.0 41.0 30.0 41.0 30-31 36.5938838196716 40.0 36.5 41.0 30.0 41.0 32-33 36.422630990783986 40.0 36.0 41.0 30.0 41.0 34-35 36.24460260084169 40.0 35.5 41.0 29.0 41.0 36-37 36.03446547586722 40.0 35.0 41.0 28.0 41.0 38-39 35.87517424956338 40.0 35.0 41.0 27.0 41.0 40-41 35.729217968510326 40.0 35.0 41.0 27.0 41.0 42-43 35.54183807058987 40.0 35.0 41.0 26.0 41.0 44-45 35.32487746765196 40.0 35.0 41.0 24.5 41.0 46-47 34.982553288284095 40.0 35.0 41.0 23.5 41.0 48-49 34.65477712303575 39.0 35.0 41.0 21.0 41.0 50-51 34.426523275776134 39.0 34.5 41.0 19.5 41.0 52-53 34.22819849496845 39.0 34.0 41.0 18.0 41.0 54-55 34.015533890748344 39.0 34.0 41.0 15.5 41.0 56-57 33.77717453265868 38.0 34.0 41.0 13.5 41.0 58-59 33.53384200528408 38.0 33.5 40.5 11.0 41.0 60-61 33.24959146704501 37.5 33.0 40.0 8.0 41.0 62-63 32.96525197960172 37.0 33.0 40.0 7.0 41.0 64-65 32.65883821874675 36.5 33.0 40.0 4.0 41.0 66-67 32.32467974155712 36.0 32.5 39.0 2.0 41.0 68-69 31.963867346168822 35.5 32.0 39.0 2.0 41.0 70-71 31.57656819099349 35.0 32.0 38.5 2.0 40.5 72-73 31.192600637900732 35.0 31.0 37.5 2.0 40.0 74-75 30.87444888670295 35.0 31.0 37.0 2.0 39.0 76-77 30.56683142005339 35.0 31.0 36.5 2.0 39.0 78-79 30.26106756669676 35.0 31.0 36.0 2.0 38.5 80-81 30.2144325729232 35.0 31.0 36.0 2.0 37.0 82-83 30.00184149637346 35.0 31.0 35.5 2.0 37.0 84-85 29.792769834157927 35.0 31.0 35.0 2.0 37.0 86-87 29.61249182796398 35.0 31.0 35.0 2.0 36.0 88-89 29.432605969345296 35.0 31.0 35.0 2.0 36.0 90-91 29.272029248040504 35.0 30.5 35.0 2.0 36.0 92-93 29.15983566152597 35.0 30.0 35.0 2.0 35.0 94-95 29.043945863806925 35.0 30.0 35.0 2.0 35.0 96-97 28.97430304305417 35.0 30.0 35.0 2.0 35.0 98-99 28.883152139489976 35.0 30.0 35.0 2.0 35.0 100-101 28.780285000961094 35.0 30.0 35.0 2.0 35.0 102-103 27.470264253490363 32.5 27.0 34.5 2.0 35.0 104-105 28.46428290142941 34.0 29.0 35.0 2.0 35.0 106-107 28.614593287336774 34.0 30.0 35.0 2.0 35.0 108-109 28.60540756084759 34.0 30.0 35.0 2.0 35.0 110-111 28.50768130078435 34.0 29.5 35.0 2.0 35.0 112-113 28.37282521949524 34.0 29.0 35.0 2.0 35.0 114-115 28.262488550888033 34.0 29.0 35.0 2.0 35.0 116-117 28.135855176375532 34.0 29.0 35.0 2.0 35.0 118-119 27.96640749310299 34.0 29.0 35.0 2.0 35.0 120-121 28.069671185359677 35.0 29.0 35.0 2.0 35.0 122-123 27.94276049862225 35.0 29.0 35.0 2.0 35.0 124-125 27.23540723589384 34.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31800.0 3 12883.0 4 5658.0 5 5279.0 6 6569.0 7 7829.0 8 8126.0 9 9077.0 10 10608.0 11 12174.0 12 13409.0 13 15989.0 14 21542.0 15 19997.0 16 10741.0 17 8930.0 18 8798.0 19 8688.0 20 8554.0 21 8656.0 22 8024.0 23 8840.0 24 11058.0 25 14340.0 26 17353.0 27 20756.0 28 24506.0 29 29504.0 30 35629.0 31 43377.0 32 54112.0 33 69580.0 34 96137.0 35 146941.0 36 279575.0 37 509103.0 38 210979.0 39 522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81755608617166 18.848230797410086 12.959679305824665 24.374533810593586 2 16.449160086018473 21.264619174363673 37.73625334907989 24.54996739053797 3 18.903110358148602 24.863588271482882 30.5715936447859 25.661707725582616 4 12.89019340439993 16.935029913404797 36.42010735998643 33.75466932220885 5 13.112794711220785 38.02473847878101 34.53514141395199 14.327325396046211 6 31.97842210394959 36.88752022858856 16.7037983446731 14.430259322788746 7 27.085597388283517 33.28269561572472 21.128817175990672 18.50288982000109 8 26.37293935160908 34.33835615231061 20.19617196416532 19.092532531914987 9 26.441057199419642 14.842588950985933 19.46042773148959 39.25592611810483 10-11 24.957478637421662 25.827293867780593 27.92684122457118 21.288386270226564 12-13 26.002518345030897 23.588140423623972 27.8449256889921 22.564415542353032 14-15 23.291198785537944 24.891184243720854 26.04403636776255 25.773580602978647 16-17 22.401473178825093 27.42209129257891 26.984114743135653 23.192320785460346 18-19 22.235341978049703 26.019588190236643 29.497581291760692 22.24748853995296 20-21 23.372198396368553 25.255184533302483 29.37490875937541 21.997708310953556 22-23 22.775447626408685 25.343134567917218 29.111974982557147 22.76944282311695 24-25 22.583861689568376 25.35928132166827 29.423800187415196 22.633056801348157 26-27 22.460866379654647 25.48862281056931 29.423276619538303 22.627234190237736 28-29 22.370215023376417 25.622668762198458 29.46604704149362 22.5410691729315 30-31 22.893330112456173 25.459987257886763 29.241304389142925 22.405378240514136 32-33 22.509868581428087 25.558407045276066 29.35304910313043 22.578675270165412 34-35 22.581986832747468 25.589653498863246 29.482068969696368 22.34629069869292 36-37 22.85500303954047 25.4250467499779 29.161216289953707 22.558733920527928 38-39 22.665382054932575 25.465031375760226 29.182197550919682 22.68738901838752 40-41 22.779469191915993 25.50788102887071 29.110322284403463 22.602327494809842 42-43 22.82569295553053 25.395561426742496 28.990512695646043 22.78823292208093 44-45 22.816662722910596 25.63454300590241 28.665771769189174 22.883022501997818 46-47 22.856286597146436 25.55788526131788 28.29548540851905 23.29034273301664 48-49 22.910645639562187 25.685140437918086 28.124160797470665 23.28005312504906 50-51 22.81496080883708 26.092151218755795 27.839841048498222 23.25304692390891 52-53 22.844654378197493 26.090407695326757 27.61958535023514 23.445352576240612 54-55 22.635246795655707 26.167214220083423 27.517341027725813 23.680197956535057 56-57 22.67468249561531 26.24258568872672 27.248117227642492 23.834614588015476 58-59 22.459163267335214 26.095545932493696 27.605380652137328 23.83991014803376 60-61 22.611995733102415 26.256238051681464 27.445912556142122 23.685853659074002 62-63 22.31047661226531 26.277186905777032 27.585661750934783 23.826674731022873 64-65 22.24613760717851 26.066909578910487 27.574845911956615 24.112106901954387 66-67 22.237585457614315 26.253669846948206 27.45471066636075 24.05403402907673 68-69 22.560054601101275 25.967390274481804 27.391580016613894 24.08097510780302 70-71 22.247012496501817 25.620280207224656 27.58306394568616 24.549643350587363 72-73 22.362366272357725 25.619099380122435 27.57660458708849 24.441929760431353 74-75 22.368351108879548 25.681507638807215 27.380446449302166 24.569694803011068 76-77 22.10717069261339 25.485688633803633 27.665097782171998 24.742042891410975 78-79 22.269796192999024 25.62260437469377 27.41453279230106 24.693066640006148 80-81 22.194174479179576 25.547729730295472 27.348807975511154 24.909287815013798 82-83 22.15958479353702 25.46560345843804 27.34903210667043 25.02577964135451 84-85 22.20688131813895 25.36085552118312 27.12054259979024 25.31172056088769 86-87 21.93251390124402 25.272475399135768 27.29033839442353 25.504672305196685 88-89 22.033740979452432 25.288822784112817 27.179309205090068 25.49812703134468 90-91 21.973184952107722 25.274854516328766 27.120805982843834 25.631154548719675 92-93 21.952290968301487 25.261781078059638 27.01414921941467 25.77177873422421 94-95 21.8196515203468 25.25547967936457 27.034920949677975 25.889947850610657 96-97 21.823562869344748 24.991179107499203 27.079685505228497 26.10557251792755 98-99 21.83295143516862 25.064088103479666 26.889239065496707 26.213721395855003 100-101 21.861662655076117 25.094881599773462 26.81993899150321 26.223516753647203 102-103 21.68123092245918 25.014446735478487 26.761716729402313 26.542605612660026 104-105 21.650672239653108 25.166848075160402 26.630032433194668 26.552447251991822 106-107 21.572012013203064 25.205251259271012 26.59325876862465 26.62947795890127 108-109 21.53475578973982 25.13563258531034 26.64738612412347 26.682225500826366 110-111 21.565199475216716 25.339854469871646 26.44663156175362 26.648314493158015 112-113 21.447214035860853 25.488831354835156 26.32519899743851 26.73875561186548 114-115 21.438840559273384 25.472905272423514 26.261920749223183 26.826333419079916 116-117 21.31966735657541 25.39483849112414 26.397450780312955 26.888043371987504 118-119 21.3543168474275 25.413979076523784 26.31487774442646 26.91682633162225 120-121 21.37513518396172 25.560984180550417 26.209454106026087 26.854426529461772 122-123 21.332109466160926 25.54258157209649 26.070105777484752 27.05520318425783 124-125 21.127367189928997 25.712693978972016 26.046985933521093 27.11295289757789 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 211.0 1 150.0 2 276.0 3 610.5 4 476.0 5 220.5 6 437.5 7 978.0 8 1550.5 9 1744.0 10 1329.0 11 898.0 12 864.0 13 930.5 14 1055.0 15 1180.0 16 1384.0 17 1626.0 18 1815.5 19 2078.0 20 2410.0 21 2799.5 22 3286.0 23 3928.5 24 4731.0 25 5742.5 26 7284.0 27 9166.5 28 11008.0 29 13231.0 30 15508.0 31 18139.0 32 21837.0 33 24982.5 34 27835.5 35 31357.0 36 36298.0 37 40624.0 38 42757.0 39 44866.0 40 48513.0 41 52474.5 42 54839.0 43 57999.5 44 62427.5 45 66239.5 46 68996.5 47 71594.5 48 74597.0 49 76645.0 50 78756.5 51 78041.0 52 74232.0 53 72182.0 54 69343.5 55 63971.0 56 57928.0 57 52197.5 58 47639.5 59 42642.0 60 35958.0 61 29457.0 62 23729.5 63 17731.5 64 12502.0 65 9181.5 66 6811.0 67 5176.5 68 3904.5 69 2886.5 70 2300.5 71 1817.5 72 1411.5 73 1226.5 74 938.5 75 636.5 76 439.5 77 263.5 78 150.0 79 82.5 80 51.0 81 39.5 82 36.5 83 30.0 84 20.5 85 11.5 86 7.5 87 5.0 88 8.0 89 8.0 90 7.0 91 7.0 92 6.0 93 4.5 94 6.0 95 14.0 96 12.5 97 4.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16936148791364825 2 0.012061842553850068 3 0.0 4 0.00380030655806235 5 0.1430347265404047 6 0.07595105425460842 7 0.007270151676293192 8 0.010739996794524033 9 0.011290765860909881 10-11 0.004681537064279707 12-13 0.00908768959536649 14-15 0.010409535354692526 16-17 0.013714149753007613 18-19 0.017321687137834916 20-21 0.021369839775770897 22-23 0.023655531401272165 24-25 0.023242454601482783 26-27 0.021369839775770897 28-29 0.019387071136781845 30-31 0.022168454922030376 32-33 0.022719223988416228 34-35 0.02103937833593939 36-37 0.022113378015391795 38-39 0.016991225698003407 40-41 0.023655531401272165 42-43 0.020653839989469295 44-45 0.029603837318239325 46-47 0.024233838920977305 48-49 0.016715841164810483 50-51 0.01357645748641115 52-53 0.014072149646158412 54-55 0.016247687458382513 56-57 0.012943073060067426 58-59 0.011703842660699269 60-61 0.015008457059014355 62-63 0.013603995939730443 64-65 0.024619377267447402 66-67 0.01886384052371529 68-69 0.016247687458382513 70-71 0.022856916255012686 72-73 0.028997991345214892 74-75 0.015118610872291523 76-77 0.007049844049738852 78-79 0.012364765540362284 80-81 0.01575199529863525 82-83 0.014154765006116291 84-85 0.012943073060067426 86-87 0.008702151248896397 88-89 0.018891378977034582 90-91 0.017569533217708547 92-93 0.013356149859856812 94-95 0.010216766181457478 96-97 0.019304455776823968 98-99 0.020295840096318494 100-101 0.026767376626352205 102-103 0.019497224950059015 104-105 0.012061842553850068 106-107 0.01690861033804553 108-109 0.009280458768601537 110-111 0.022994608521609148 112-113 0.016137533645105343 114-115 0.011896611833934314 116-117 0.010216766181457478 118-119 0.006746921063226636 120-121 0.0033872297582729647 122-123 0.004764152424237584 124-125 7.986151462594794E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1815643.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.51349099665879 #Duplication Level Percentage of deduplicated Percentage of total 1 68.82431937350074 33.38907998277497 2 14.594290741622725 14.160399849926694 3 6.460127765270015 9.40210050533077 4 3.245638739741991 6.298290631155201 5 1.944212506838929 4.71602679730609 6 1.2604185091536917 3.6688381197509825 7 0.8005652207759969 2.7186749541248134 8 0.5641768575365657 2.189615111889874 9 0.4084294684582654 1.7832905406738173 >10 1.7983405920536422 14.675911895854904 >50 0.06767169847863165 2.244578620062604 >100 0.028844795827395672 2.532773111863696 >500 0.001709844658505979 0.5513445456385105 >1k 0.0010259067951035874 0.9439770706167879 >5k 2.2797928780079723E-4 0.725098263030306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6972 0.3839961930842131 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6140 0.3381722067609106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4195 0.23104762334886317 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2689 0.1481018019511545 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1898 0.10453596880003392 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1835 0.10106612368180308 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.507690663858479E-5 0.0 9 0.0 0.0 0.0 1.1015381327716958E-4 0.0 10-11 0.0 0.0 0.0 1.6523071991575436E-4 0.0 12-13 0.0 0.0 0.0 1.6523071991575436E-4 0.0 14-15 0.0 0.0 0.0 1.6523071991575436E-4 0.0 16-17 0.0 0.0 0.0 2.4784607987363155E-4 0.0 18-19 0.0 0.0 0.0 3.304614398315087E-4 0.0 20-21 0.0 0.0 0.0 3.8553834647009355E-4 0.0 22-23 0.0 0.0 0.0 8.261535995787719E-4 0.0 24-25 0.0 0.0 0.0 0.0016798456524768361 0.0 26-27 0.0 0.0 0.0 0.0032495374916765027 0.0 28-29 0.0 0.0 0.0 0.00782092074267904 0.0 30-31 0.0 0.0 0.0 0.022113378015391795 0.0 32-33 0.0 0.0 0.0 0.05601321405144073 0.0 34-35 0.0 0.0 0.0 0.11365119684871972 0.0 36-37 0.0 0.0 0.0 0.21053147562599034 0.0 38-39 0.0 0.0 0.0 0.36915296674511455 0.0 40-41 0.0 0.0 0.0 0.5596639868079794 0.0 42-43 0.0 0.0 0.0 0.7295487053346941 0.0 44-45 0.0 5.507690663858479E-5 0.0 0.9303866453922935 0.0 46-47 2.7538453319292395E-5 5.507690663858479E-5 0.0 1.1788936481455883 0.0 48-49 5.507690663858479E-5 5.507690663858479E-5 0.0 1.4475587987286045 0.0 50-51 8.261535995787719E-5 5.507690663858479E-5 0.0 1.7345920976755893 0.0 52-53 1.1015381327716958E-4 5.507690663858479E-5 0.0 2.014933552465986 0.0 54-55 1.1015381327716958E-4 5.507690663858479E-5 0.0 2.3246860754013867 0.0 56-57 1.1015381327716958E-4 5.507690663858479E-5 0.0 2.7010265784628364 0.0 58-59 1.1015381327716958E-4 5.507690663858479E-5 0.0 3.114516455052012 0.0 60-61 1.1015381327716958E-4 5.507690663858479E-5 0.0 3.553699708588087 0.0 62-63 1.1015381327716958E-4 5.507690663858479E-5 0.0 3.9894406554592505 0.0 64-65 1.1015381327716958E-4 5.507690663858479E-5 0.0 4.464671744390279 0.0 66-67 1.1015381327716958E-4 5.507690663858479E-5 0.0 5.006711121073911 0.0 68-69 1.1015381327716958E-4 5.507690663858479E-5 0.0 5.642463854403095 0.0 70-71 1.1015381327716958E-4 5.507690663858479E-5 0.0 6.3332934943708645 0.0 72-73 1.1015381327716958E-4 5.507690663858479E-5 0.0 7.014897752476671 0.0 74-75 1.1015381327716958E-4 5.507690663858479E-5 0.0 7.693858319063825 0.0 76-77 1.1015381327716958E-4 5.507690663858479E-5 0.0 8.45102809307777 0.0 78-79 1.1015381327716958E-4 5.507690663858479E-5 0.0 9.319563372314931 0.0 80-81 1.1015381327716958E-4 5.507690663858479E-5 0.0 10.248738325761177 0.0 82-83 1.1015381327716958E-4 5.507690663858479E-5 0.0 11.216219267774557 0.0 84-85 1.1015381327716958E-4 5.507690663858479E-5 0.0 12.16255067763872 0.0 86-87 1.1015381327716958E-4 5.507690663858479E-5 0.0 13.123036852509001 0.0 88-89 1.1015381327716958E-4 5.507690663858479E-5 0.0 14.178337922157604 0.0 90-91 1.1015381327716958E-4 5.507690663858479E-5 0.0 15.292791589536048 0.0 92-93 1.1015381327716958E-4 5.507690663858479E-5 0.0 16.43618817135307 0.0 94-95 1.1015381327716958E-4 5.507690663858479E-5 0.0 17.566559064750063 0.0 96-97 1.1015381327716958E-4 5.507690663858479E-5 0.0 18.713342876325356 0.0 98-99 1.1015381327716958E-4 5.507690663858479E-5 0.0 19.93098312829119 0.0 100-101 1.1015381327716958E-4 5.507690663858479E-5 0.0 21.221600281553147 0.0 102-103 1.1015381327716958E-4 5.507690663858479E-5 0.0 22.518661432891818 0.0 104-105 1.1015381327716958E-4 5.507690663858479E-5 0.0 23.79283812952216 0.0 106-107 1.1015381327716958E-4 5.507690663858479E-5 0.0 25.03413391288926 0.0 108-109 1.1015381327716958E-4 5.507690663858479E-5 0.0 26.30205938061612 0.0 110-111 1.1015381327716958E-4 5.507690663858479E-5 0.0 27.625447293328037 0.0 112-113 1.1015381327716958E-4 5.507690663858479E-5 0.0 28.985488887407932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6140 0.0 43.818096 1 GTATCAA 9270 0.0 37.067055 1 ACCGCGC 175 1.6760168E-8 30.59342 8 TCAACGC 11285 0.0 29.994974 4 TATCAAC 11525 0.0 29.680603 2 CAACGCA 11385 0.0 29.67917 5 ATCAACG 11485 0.0 29.472645 3 AACGCAG 11590 0.0 29.122877 6 CATGGGG 4595 0.0 27.62459 4 ACGCAGA 12915 0.0 25.886032 7 GAGTACT 8115 0.0 25.547583 12-13 CGCAGAG 13195 0.0 25.336725 8 TACATGG 8865 0.0 25.208921 2 GTACATG 9160 0.0 25.138374 1 ACATGGG 8800 0.0 24.988802 3 AGTACTT 8445 0.0 24.020954 12-13 TTAGCGC 125 0.001618962 23.837557 4 GCGCCGT 150 1.6813944E-4 23.811934 6 GCAGAGT 13900 0.0 23.709291 9 GTACTTT 8955 0.0 22.985714 14-15 >>END_MODULE