FastQCFastQC Report
Mon 15 Aug 2016
SRR2921080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5532326
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107000.037.9662441
GGTATCA71400.037.8472441
GTACATG194150.033.183381
TCAACGC120650.032.7589844
TACATGG198900.031.930922
AACGCAG123750.031.7416486
CAACGCA124350.031.592785
ATCAACG129000.030.638543
TATCAAC133250.030.1074852
ACATGGG210050.029.358023
CATGGGG147100.028.5276574
ACGCAGA146050.026.608167
CGCAGAG151650.025.5466488
GCAGAGT172850.021.4493439
AGAGTAC142850.020.63887810-11
CAGAGTA156950.019.35339210-11
ATGGGGG73800.019.1959785
GTATAGA20250.019.1479641
ATGGGGA77950.018.7848915
GTATATA23400.018.0999241
Produced by FastQC (version 0.11.5)