FastQCFastQC Report
Mon 15 Aug 2016
SRR2921080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5532326
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101000.18256335581091931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90930.16436124696917717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59650.10782083340714196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.059.652951
GTATCAA146300.045.9119761
TCAACGC182650.036.3489724
ATCAACG186750.035.6466833
CAACGCA188800.035.228065
TATCAAC194500.034.410792
AACGCAG197050.033.593236
GTACATG184350.029.9963551
TACATGG183050.029.6933752
ACGCAGA226450.029.0010577
CGCAGAG231950.028.1600158
ACATGGG193650.026.9903583
CATGGGG137000.026.5795274
GCAGAGT257600.024.593759
GAGTACT153600.023.02446412-13
CAGAGTA243400.022.9855710-11
AGAGTAC231700.022.82405710-11
GTACTTT162450.021.8079314-15
AGTACTT162300.020.80061712-13
TACTTTT184850.018.34458214-15
Produced by FastQC (version 0.11.5)