FastQCFastQC Report
Mon 15 Aug 2016
SRR2921079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098526
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104950.032.8492361
GTACATG175500.031.4712311
TACATGG177650.030.457682
CATGGGG124200.029.9069234
ACATGGG176500.029.6113743
GGTATCA77250.028.877061
TCAACGC119550.028.1324564
AACGCAG121250.027.7332786
CAACGCA123950.027.0377545
TATCAAC127050.026.7990552
ATCAACG126600.026.6128583
ACGCAGA136400.024.302977
CGCAGAG142950.023.1891238
ATGGGGG64150.022.4557445
ATGGGGA63200.020.8153615
GTATATA16050.020.0677451
GCAGAGT159000.019.912599
AGAGTAC128900.019.31850810-11
ATGGGGC48750.018.3157795
GTGTACG6750.017.6728291
Produced by FastQC (version 0.11.5)