##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921079_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4098526
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.956281111794826	31.0	31.0	34.0	26.0	34.0
2	29.988995311973135	31.0	31.0	34.0	25.0	34.0
3	30.108765443966927	31.0	31.0	34.0	26.0	34.0
4	33.52888379871202	37.0	35.0	37.0	28.0	37.0
5	33.574374055453106	37.0	35.0	37.0	28.0	37.0
6	33.636310224700296	37.0	35.0	37.0	28.0	37.0
7	33.52764725659908	37.0	35.0	37.0	28.0	37.0
8	33.49942296327997	37.0	35.0	37.0	27.0	37.0
9	34.95382681481098	39.0	35.0	39.0	27.0	39.0
10-11	34.916875359580494	39.0	35.0	39.0	27.0	39.0
12-13	34.84791056589613	39.0	35.0	39.0	27.0	39.0
14-15	36.05481531653087	40.0	36.0	41.0	27.0	41.0
16-17	36.03071823870337	40.0	36.0	41.0	27.0	41.0
18-19	36.04832920420658	40.0	36.0	41.0	27.0	41.0
20-21	36.10912996526068	40.0	36.0	41.0	27.0	41.0
22-23	36.036120302762505	40.0	36.0	41.0	27.0	41.0
24-25	36.01996510452783	40.0	36.0	41.0	27.0	41.0
26-27	35.9660568214036	40.0	36.0	41.0	27.0	41.0
28-29	35.83842349664245	40.0	36.0	41.0	27.0	41.0
30-31	35.735458796650306	40.0	36.0	41.0	27.0	41.0
32-33	35.6287370142339	40.0	35.0	41.0	26.0	41.0
34-35	35.51637625331644	40.0	35.0	41.0	25.5	41.0
36-37	35.37932198063402	40.0	35.0	41.0	25.0	41.0
38-39	35.23952647854375	40.0	35.0	41.0	24.5	41.0
40-41	35.10446145760696	40.0	35.0	41.0	24.0	41.0
42-43	34.906696456238166	39.5	35.0	41.0	23.5	41.0
44-45	34.6976713091487	39.0	34.0	41.0	23.0	41.0
46-47	34.511501573980496	39.0	34.0	41.0	22.5	41.0
48-49	34.27289676825278	39.0	34.0	41.0	21.0	41.0
50-51	34.08688147885361	38.5	33.0	40.5	20.0	41.0
52-53	33.890255545530266	38.0	33.0	40.0	18.5	41.0
54-55	33.646956735177476	38.0	33.0	40.0	18.0	41.0
56-57	33.39301897804235	38.0	33.0	40.0	16.0	41.0
58-59	33.0977566081074	37.0	32.5	40.0	13.5	41.0
60-61	32.77904751610701	37.0	32.0	40.0	11.0	41.0
62-63	32.47987971773267	36.0	32.0	39.5	8.0	41.0
64-65	32.13915600389018	36.0	31.0	39.0	7.5	41.0
66-67	31.784952443878602	35.0	31.0	39.0	7.0	40.5
68-69	31.4163687140206	35.0	31.0	38.5	4.0	40.0
70-71	31.050917817771563	35.0	30.5	37.5	2.0	40.0
72-73	30.703009203796682	35.0	30.0	37.0	2.0	39.0
74-75	30.653765036503366	35.0	31.0	36.5	2.0	39.0
76-77	30.708088712868967	35.0	31.0	36.0	2.0	39.0
78-79	30.537661954566104	35.0	31.0	36.0	2.0	38.0
80-81	30.329814181976644	35.0	31.0	35.5	2.0	37.0
82-83	30.094032342359178	35.0	31.0	35.0	2.0	37.0
84-85	29.849470272971306	35.0	31.0	35.0	2.0	36.0
86-87	29.711798949183194	35.0	31.0	35.0	2.0	36.0
88-89	29.542338635890076	35.0	31.0	35.0	2.0	36.0
90-91	29.40284372967257	35.0	30.5	35.0	2.0	35.5
92-93	29.29106915510601	35.0	30.0	35.0	2.0	35.0
94-95	29.181529042392313	35.0	30.0	35.0	2.0	35.0
96-97	29.08572240361535	35.0	30.0	35.0	2.0	35.0
98-99	28.98126863169832	35.0	30.0	35.0	2.0	35.0
100-101	28.853909673868117	34.0	30.0	35.0	2.0	35.0
102-103	27.53625120348145	32.5	27.0	34.5	2.0	35.0
104-105	28.504341072863756	34.0	29.0	35.0	2.0	35.0
106-107	28.64607824861914	34.0	30.0	35.0	2.0	35.0
108-109	28.58004121481723	34.0	29.5	35.0	2.0	35.0
110-111	28.482303271956795	34.0	29.0	35.0	2.0	35.0
112-113	28.370747678555656	34.0	29.0	35.0	2.0	35.0
114-115	28.229937909384986	34.0	29.0	35.0	2.0	35.0
116-117	28.092742976377362	34.0	29.0	35.0	2.0	35.0
118-119	27.895425940935837	34.0	29.0	35.0	2.0	35.0
120-121	27.69674634246556	34.0	29.0	35.0	2.0	35.0
122-123	27.404555808600456	34.0	28.5	35.0	2.0	35.0
124-125	26.153854092910475	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143096.0
3	21903.0
4	14761.0
5	15073.0
6	20046.0
7	21043.0
8	19371.0
9	20414.0
10	21848.0
11	22984.0
12	23367.0
13	22938.0
14	23593.0
15	21748.0
16	17349.0
17	15672.0
18	15052.0
19	14979.0
20	15444.0
21	16437.0
22	19459.0
23	25198.0
24	34791.0
25	44549.0
26	50325.0
27	54426.0
28	63672.0
29	75369.0
30	88381.0
31	108263.0
32	135773.0
33	176484.0
34	245174.0
35	372312.0
36	699487.0
37	1069804.0
38	327558.0
39	383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.276468971942904	17.382915196477466	11.86425251017908	25.476363321400548
2	16.437562502929826	20.415751910245795	37.54760965357751	25.599075933246873
3	19.352331568262716	24.144002609758562	28.5722901656933	27.93137565628542
4	13.172084168772205	16.294824637809626	34.880381758630264	35.6527094347879
5	14.232679584057509	37.668285837993814	33.37059112411484	14.728443453833837
6	33.62034407577729	35.288299347147664	16.806776472887393	14.284580104187661
7	28.95021729614807	32.2429036784017	20.363086816845925	18.4437922086043
8	27.000557157223128	34.67242403939231	19.27519731939227	19.051821483992292
9	26.823085177664257	15.840934534282777	18.293227181557498	39.04275310649547
10-11	25.60360210525776	25.833758587976302	26.962597495656176	21.60004181110976
12-13	26.094787840900214	23.487853598499647	26.791887258732515	23.625471301867627
14-15	23.56196500311382	24.7395870220898	24.020099397994947	27.678348576801437
16-17	23.835280714061987	27.412648724754114	24.792150153769505	23.959920407414394
18-19	23.94177521561332	26.65205658224882	26.090173151234765	23.315995050903098
20-21	24.887986636175345	25.794826316556147	25.7421270737964	23.575059973472108
22-23	24.640483211074123	25.537738473823683	25.58111427588841	24.240664039213776
24-25	24.24732211415838	25.85830864057498	25.628879349446905	24.265489895819737
26-27	24.04053280973061	25.910153533555203	25.69756203587191	24.351751620842272
28-29	24.17431266593601	26.148413949662903	25.640593589375406	24.03667979502568
30-31	24.29745809997888	26.025532548749563	25.457802066583398	24.219207284688164
32-33	23.940313539152953	26.166217095868294	25.515701928150158	24.3777674368286
34-35	24.21167879468521	26.099879293676313	25.603696451068032	24.08474546057045
36-37	24.389831746013915	25.93082297471172	25.475029785895636	24.20431549337873
38-39	24.232215540394776	26.070542175418908	25.499394264811574	24.197848019374735
40-41	24.150653163386025	26.086657107751233	25.6437829422078	24.118906786654946
42-43	24.14540709498602	26.042133911677357	25.47594589110486	24.336513102231763
44-45	24.074998754733233	26.057854117242595	25.566950424709216	24.30019670331495
46-47	24.16963843090848	26.07983595348679	25.48856341256397	24.261962203040763
48-49	24.101547998803174	26.15347316720284	25.356749469857988	24.388229364135995
50-51	23.909657919856123	26.305731481010124	25.406622496182905	24.377988102950848
52-53	24.1032859481966	26.00609019450847	25.305855790025156	24.584768067269778
54-55	24.00454352760988	26.081218425340214	25.344427524312245	24.56981052273766
56-57	23.908614410569665	26.139673178073075	25.312135393294444	24.63957701806282
58-59	24.01740514634703	25.82532840647328	25.411611324942047	24.745655122237643
60-61	23.903555306270004	25.866044125658476	25.579932795459335	24.650467772612185
62-63	23.864381441146513	25.82686842851348	25.58145071902505	24.727299411314956
64-65	23.80015556495093	25.877635017179664	25.508171310927114	24.814038106942295
66-67	23.796528511369644	25.841761950356485	25.496051208846133	24.86565832942774
68-69	23.878439291679282	25.624004961761234	25.65095035308596	24.846605393473524
70-71	23.660147827317395	25.566536882416347	25.746226882371175	25.027088407895082
72-73	23.70812428178265	25.595124019824812	25.694890162030564	25.001861536361968
74-75	23.60804231904953	25.652895686141203	25.86147993596074	24.877582058848525
76-77	23.63787751465808	25.369544263135	26.006980490817593	24.985597731389326
78-79	23.55186222715223	25.513819501298112	26.043064269953287	24.891254001596366
80-81	23.629145651580412	25.438392566288183	26.03524380756001	24.8972179745714
82-83	23.502802687092352	25.418193002800184	26.120137697792835	24.95886661231463
84-85	23.466214444001057	25.442421468724007	26.034815902751284	25.056548184523646
86-87	23.413075933930745	25.32302309669473	26.143577704532454	25.120323264842064
88-89	23.412344895355865	25.229753236369408	26.053368814887605	25.304533053387125
90-91	23.34249232734824	25.299006315896406	26.138138500399506	25.220362856355848
92-93	23.462033268794766	25.200595068286734	26.156320049277337	25.18105161364116
94-95	23.225366725410133	25.116745160196523	26.33453347785374	25.32335463653961
96-97	23.195829152299353	25.033778929102645	26.33203499993957	25.438356918658435
98-99	23.125827180714197	25.242823816260984	26.16228313535933	25.469065867665492
100-101	23.04305403827658	25.287692170750937	26.321255758757996	25.34799803221449
102-103	22.843248206524056	25.353131870606795	26.349646419523896	25.453973503345257
104-105	22.699724349755968	25.452308113004392	26.208124479646074	25.639843057593563
106-107	22.667712641748448	25.537534530913753	26.25909860960608	25.535654217731718
108-109	22.609165176159834	25.521697741821097	26.331760934100874	25.537376147918188
110-111	22.60186020536079	25.75170896920223	26.276227932237312	25.370202893199668
112-113	22.61489206679728	25.7410335646769	26.260067156700924	25.384007211824894
114-115	22.57562248792973	25.823966374430736	26.26262552262173	25.337785615017804
116-117	22.390484623063355	25.72411179461734	26.37688439020674	25.508519192112566
118-119	22.484183611118944	25.698041113027436	26.264978429450075	25.552796846403545
120-121	22.461308757997973	25.771910565585976	26.357916688089034	25.408863988327017
122-123	22.397137741606528	25.86675793047671	26.255308799224824	25.480795528691935
124-125	22.41879591192726	25.929522657618538	26.07857308245804	25.573108347996165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2827.0
1	2122.5
2	1269.5
3	1055.0
4	861.0
5	686.0
6	593.5
7	597.0
8	657.0
9	722.5
10	772.0
11	764.5
12	811.0
13	918.0
14	1049.5
15	1218.5
16	1388.5
17	1643.5
18	1989.5
19	2372.5
20	2909.0
21	3646.0
22	4586.0
23	5758.5
24	7088.0
25	9052.5
26	11913.5
27	15390.0
28	18469.0
29	21957.5
30	26329.0
31	31711.5
32	38147.0
33	43191.5
34	47684.0
35	52922.5
36	59091.0
37	66292.0
38	74668.0
39	82896.5
40	90901.5
41	99388.5
42	107031.5
43	116693.5
44	125698.5
45	133840.0
46	143790.5
47	153072.5
48	162369.0
49	170036.0
50	176448.0
51	180805.5
52	180458.0
53	181212.0
54	180565.0
55	172742.5
56	162331.5
57	150062.5
58	138226.0
59	125452.0
60	106561.0
61	88637.0
62	73432.0
63	56869.0
64	42640.0
65	32580.0
66	24662.5
67	18330.5
68	13581.5
69	10028.5
70	7731.5
71	5969.5
72	4550.0
73	3813.5
74	3117.0
75	2364.0
76	1717.5
77	1159.5
78	800.5
79	532.0
80	362.0
81	274.0
82	202.0
83	146.5
84	112.0
85	89.5
86	86.0
87	80.0
88	55.5
89	37.0
90	30.5
91	27.0
92	24.5
93	25.5
94	26.0
95	22.5
96	15.0
97	13.0
98	15.0
99	21.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27978351241397514
2	0.06631652452613451
3	0.07632012094104075
4	0.08229787977433839
5	0.0886416238423277
6	0.12079952646390435
7	0.11160109756531982
8	0.11050314186124474
9	0.11626130955372736
10-11	0.09565634084058512
12-13	0.08505496854234913
14-15	0.09457058464433311
16-17	0.09572953788752346
18-19	0.08875141941273522
20-21	0.08760466567736791
22-23	0.07135492125705681
24-25	0.08209048814134642
26-27	0.09316764124468163
28-29	0.08720208191920706
30-31	0.08200509158658503
32-33	0.09014216330456365
34-35	0.08449379118248854
36-37	0.08459138724507298
38-39	0.07538075883866542
40-41	0.08711668536444565
42-43	0.07697889436348579
44-45	0.07363622921996835
46-47	0.06379122640676185
48-49	0.0656211525802203
50-51	0.06842703937952327
52-53	0.07940659642027402
54-55	0.08466458429201132
56-57	0.09243567077529824
58-59	0.06370582985200045
60-61	0.07971158411585043
62-63	0.08459138724507298
64-65	0.07638111848015604
66-67	0.07089133995978066
68-69	0.07822324416143756
70-71	0.07660070962097106
72-73	0.05970439128603795
74-75	0.08632371735594699
76-77	0.08991037265592558
78-79	0.07253827351589327
80-81	0.08422540201038128
82-83	0.08823904008416684
84-85	0.09242347126747517
86-87	0.08071194375734106
88-89	0.061021938130928044
90-91	0.08446939216684242
92-93	0.06161971401425781
94-95	0.08789745386512127
96-97	0.07679590174613996
98-99	0.07106213306930345
100-101	0.08704348831750733
102-103	0.060985339607458874
104-105	0.0672436871206868
106-107	0.08450599069031159
108-109	0.09081313623483174
110-111	0.08394481333045099
112-113	0.0739290174077217
114-115	0.086543308496762
116-117	0.08056554966346438
118-119	0.07347763561826862
120-121	0.1045619815514163
122-123	0.08388381579133572
124-125	0.10428139287148598
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4098526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.401061061406324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18712218915819	30.275805759223868
2	13.802195630592982	12.256642619508689
3	6.241182447862868	8.313453688888098
4	3.430345616295346	6.092439406834286
5	2.0985384896226895	4.658866780872422
6	1.4089329704834868	3.753487131231953
7	0.9461502682736609	2.9407053084419355
8	0.7031683491522464	2.497713664572574
9	0.5171296615643357	2.0664995111804214
>10	2.4961155952425185	19.053922121826496
>50	0.11901179114002494	3.5835031973550486
>100	0.047846337046953076	3.5513379122544015
>500	0.0015438610301816153	0.45866669762460777
>1k	7.167925345089553E-4	0.49695620018513337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4399015646112774E-5
2	0.0	0.0	0.0	0.0	2.4399015646112774E-5
3	0.0	0.0	0.0	0.0	2.4399015646112774E-5
4	0.0	0.0	0.0	0.0	4.879803129222555E-5
5	0.0	0.0	0.0	0.0	4.879803129222555E-5
6	0.0	0.0	0.0	0.0	9.75960625844511E-5
7	0.0	0.0	0.0	0.0	9.75960625844511E-5
8	0.0	0.0	0.0	0.0	1.2199507823056387E-4
9	0.0	0.0	0.0	2.4399015646112774E-5	1.4639409387667664E-4
10-11	0.0	0.0	0.0	4.879803129222555E-5	1.4639409387667664E-4
12-13	0.0	0.0	0.0	1.3419458605362026E-4	4.3918228163002994E-4
14-15	0.0	0.0	0.0	1.4639409387667664E-4	6.099753911528193E-4
16-17	0.0	0.0	0.0	1.7079310952278941E-4	7.807685006756088E-4
18-19	0.0	0.0	0.0	2.4399015646112775E-4	8.783645632600599E-4
20-21	0.0	0.0	0.0	2.927881877533533E-4	0.0010003596414906238
22-23	0.0	0.0	0.0	4.513817894530863E-4	0.0011833522588364695
24-25	0.0	0.0	0.0	8.295665319678343E-4	0.001354145368359259
26-27	0.0	0.0	0.0	0.001549337493528161	0.0014395419231206536
28-29	0.0	0.0	0.0	0.004709010019699766	0.0014639409387667663
30-31	0.0	0.0	0.0	0.015249384778820485	0.0014761404465898228
32-33	1.2199507823056387E-5	0.0	0.0	0.041136740379346136	0.001573736509174274
34-35	2.4399015646112774E-5	0.0	0.0	0.0819318945396467	0.0016347340482895558
36-37	2.4399015646112774E-5	0.0	0.0	0.14807762595625842	0.0016347340482895558
38-39	2.4399015646112774E-5	0.0	0.0	0.25428654106378734	0.001671332571758725
40-41	2.4399015646112774E-5	0.0	0.0	0.3793070972344692	0.0017079310952278941
42-43	2.4399015646112774E-5	0.0	0.0	0.49925265815075953	0.0017323301108740068
44-45	2.4399015646112774E-5	0.0	0.0	0.6438778233930931	0.001829926173458458
46-47	2.4399015646112774E-5	0.0	0.0	0.8201241129127886	0.0019397217438659655
48-49	2.4399015646112774E-5	0.0	0.0	1.0102168438116532	0.0021227143612118113
50-51	2.4399015646112774E-5	0.0	0.0	1.213777831347172	0.002147113376857924
52-53	2.4399015646112774E-5	0.0	0.0	1.4182293829537742	0.002342305502026826
54-55	2.4399015646112774E-5	0.0	0.0	1.6472629428238348	0.0024643005802573903
56-57	2.4399015646112774E-5	0.0	0.0	1.9146273562739384	0.0024886995959035032
58-59	2.4399015646112774E-5	0.0	0.0	2.214784046752418	0.002525298119372672
60-61	2.4399015646112774E-5	0.0	0.0	2.53698524786716	0.0025374976271957286
62-63	2.4399015646112774E-5	0.0	0.0	2.869019252287286	0.0026350936897801794
64-65	2.4399015646112774E-5	0.0	0.0	3.215509673477733	0.002647293197603236
66-67	2.4399015646112774E-5	0.0	0.0	3.6209847149926584	0.0026716922132493486
68-69	3.659852346916916E-5	0.0	0.0	4.080991556476645	0.0026838917210724052
70-71	4.879803129222555E-5	0.0	0.0	4.589320648447758	0.002781487783656856
72-73	4.879803129222555E-5	0.0	0.0	5.104615659385837	0.00303767744794104
74-75	4.879803129222555E-5	0.0	0.0	5.62443668772627	0.003098674987056322
76-77	4.879803129222555E-5	0.0	0.0	6.200863432365685	0.0031352735105254913
78-79	4.879803129222555E-5	0.0	0.0	6.8475471425580805	0.0031840715418177167
80-81	4.879803129222555E-5	0.0	0.0	7.532439711252289	0.0032450690809329987
82-83	4.879803129222555E-5	0.0	0.0	8.253711212274851	0.0033914631748096754
84-85	4.879803129222555E-5	0.0	0.0	8.971725444708659	0.0033914631748096754
86-87	4.879803129222555E-5	0.0	0.0	9.719970057527998	0.0034280616982788445
88-89	4.879803129222555E-5	0.0	0.0	10.531273926284719	0.00347685972957107
90-91	4.879803129222555E-5	0.0	0.0	11.4010256370217	0.0034890592373941266
92-93	4.879803129222555E-5	0.0	0.0	12.289845178486118	0.0035134582530402395
94-95	4.879803129222555E-5	0.0	0.0	13.18925389274095	0.003635453331270803
96-97	4.879803129222555E-5	0.0	0.0	14.092334658850522	0.0037452489016783106
98-99	4.879803129222555E-5	0.0	0.0	15.03072080060002	0.0038062464407935927
100-101	4.879803129222555E-5	0.0	0.0	16.038522141862707	0.0038062464407935927
102-103	4.879803129222555E-5	0.0	0.0	17.069124363246686	0.0038672439799088748
104-105	4.879803129222555E-5	0.0	0.0	18.095151769197024	0.0039648400424933256
106-107	4.879803129222555E-5	1.2199507823056387E-5	0.0	19.11191974870966	0.003977039550316382
108-109	4.879803129222555E-5	2.4399015646112774E-5	0.0	20.148536327450405	0.004013638073785551
110-111	4.879803129222555E-5	2.4399015646112774E-5	0.0	21.22732660473546	0.004147832659839172
112-113	4.879803129222555E-5	2.4399015646112774E-5	0.0	22.34565548687504	0.00422102970677751
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10495	0.0	32.849236	1
GTACATG	17550	0.0	31.471231	1
TACATGG	17765	0.0	30.45768	2
CATGGGG	12420	0.0	29.906923	4
ACATGGG	17650	0.0	29.611374	3
GGTATCA	7725	0.0	28.87706	1
TCAACGC	11955	0.0	28.132456	4
AACGCAG	12125	0.0	27.733278	6
CAACGCA	12395	0.0	27.037754	5
TATCAAC	12705	0.0	26.799055	2
ATCAACG	12660	0.0	26.612858	3
ACGCAGA	13640	0.0	24.30297	7
CGCAGAG	14295	0.0	23.189123	8
ATGGGGG	6415	0.0	22.455744	5
ATGGGGA	6320	0.0	20.815361	5
GTATATA	1605	0.0	20.067745	1
GCAGAGT	15900	0.0	19.91259	9
AGAGTAC	12890	0.0	19.318508	10-11
ATGGGGC	4875	0.0	18.315779	5
GTGTACG	675	0.0	17.672829	1
>>END_MODULE