FastQCFastQC Report
Mon 15 Aug 2016
SRR2921079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098526
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95670.2334253826863609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85150.20775761822665026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.13609770927401704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92050.047.630581
GTATCAA144500.043.428041
TCAACGC182700.034.0545654
ATCAACG186050.033.601543
TATCAAC187900.033.3341452
CAACGCA187750.033.1385845
AACGCAG193200.032.3208666
GTACATG172350.029.2114751
ACGCAGA216050.028.7733487
TACATGG175900.028.222312
ACATGGG175950.027.6384873
CATGGGG124650.027.586524
CGCAGAG224950.027.4233918
GCAGAGT243100.024.8862279
GAGTACT128400.024.09165812-13
AGTACTT136150.024.05293312-13
AGAGTAC210800.023.85888910-11
GTACTTT137650.022.6903214-15
CAGAGTA231550.021.8749510-11
TACTTTT152400.021.17738314-15
Produced by FastQC (version 0.11.5)