##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4098526
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36331598238001	33.0	31.0	34.0	28.0	34.0
2	31.41897086903926	33.0	31.0	34.0	28.0	34.0
3	31.504434521093682	34.0	31.0	34.0	28.0	34.0
4	35.001560805030884	37.0	35.0	37.0	32.0	37.0
5	34.762522916775445	37.0	35.0	37.0	32.0	37.0
6	34.532659790373415	37.0	35.0	37.0	31.0	37.0
7	34.4782107030674	37.0	35.0	37.0	30.0	37.0
8	34.468030457779214	37.0	35.0	37.0	30.0	37.0
9	36.03149668929757	39.0	37.0	39.0	30.0	39.0
10-11	35.99347460526053	39.0	37.0	39.0	30.0	39.0
12-13	35.9383710143598	39.0	37.0	39.0	30.0	39.0
14-15	37.19864373191728	40.0	37.0	41.0	31.0	41.0
16-17	37.195256416575134	40.0	37.0	41.0	31.0	41.0
18-19	37.21814330322657	40.0	37.5	41.0	31.0	41.0
20-21	37.227225348820525	40.0	38.0	41.0	31.0	41.0
22-23	37.19145565991286	40.0	37.0	41.0	31.0	41.0
24-25	37.14915520848227	40.0	37.0	41.0	31.0	41.0
26-27	37.03366539580327	40.0	37.0	41.0	30.0	41.0
28-29	36.90713624849519	40.0	37.0	41.0	30.0	41.0
30-31	36.77025447685338	40.0	37.0	41.0	30.0	41.0
32-33	36.642853796706426	40.0	36.0	41.0	30.0	41.0
34-35	36.501225318565744	40.0	36.0	41.0	30.0	41.0
36-37	36.33018797489635	40.0	36.0	41.0	29.0	41.0
38-39	36.188478004043404	40.0	35.0	41.0	29.0	41.0
40-41	36.04924685118503	40.0	35.0	41.0	28.0	41.0
42-43	35.89534030039093	40.0	35.0	41.0	27.5	41.0
44-45	35.71151836050326	40.0	35.0	41.0	27.0	41.0
46-47	35.43000568985045	40.0	35.0	41.0	25.5	41.0
48-49	35.15877671631216	39.0	35.0	41.0	24.0	41.0
50-51	34.95029639924207	39.0	35.0	41.0	24.0	41.0
52-53	34.73229973409953	39.0	34.5	41.0	23.0	41.0
54-55	34.5104827930822	39.0	34.0	41.0	23.0	41.0
56-57	34.25648025656053	38.0	34.0	41.0	22.0	41.0
58-59	33.98876864511778	38.0	34.0	40.0	22.0	41.0
60-61	33.68559245445802	37.0	33.5	40.0	20.5	41.0
62-63	33.383652927906276	37.0	33.0	40.0	20.0	41.0
64-65	33.05573759932229	36.0	33.0	39.5	19.5	41.0
66-67	32.71039527381308	36.0	33.0	39.0	18.0	41.0
68-69	32.35007231868238	35.0	32.5	39.0	16.0	40.5
70-71	31.966815630790194	35.0	32.0	37.5	13.0	40.0
72-73	31.596806266447985	35.0	32.0	37.0	11.0	39.5
74-75	31.28278568929415	35.0	32.0	37.0	8.0	39.0
76-77	30.9962218124272	35.0	31.5	36.0	7.5	39.0
78-79	30.712287661466583	35.0	31.0	36.0	7.0	37.5
80-81	30.687276840503145	35.0	32.0	35.5	7.0	37.0
82-83	30.503741345059176	35.0	32.0	35.0	3.5	37.0
84-85	30.319719821223533	35.0	31.0	35.0	2.0	36.0
86-87	30.165821810084893	35.0	31.0	35.0	2.0	36.0
88-89	30.00268620962756	35.0	31.0	35.0	2.0	36.0
90-91	29.858001022806736	35.0	31.0	35.0	2.0	35.5
92-93	29.757555277189898	35.0	31.0	35.0	2.0	35.0
94-95	29.661322265614515	35.0	31.0	35.0	2.0	35.0
96-97	29.59576699037654	35.0	31.0	35.0	2.0	35.0
98-99	29.503914456075186	35.0	31.0	35.0	2.0	35.0
100-101	29.411456216210414	35.0	31.0	35.0	2.0	35.0
102-103	28.079922025625798	32.5	28.0	34.5	2.0	35.0
104-105	29.10124261746784	34.0	30.0	35.0	2.0	35.0
106-107	29.26784446408294	34.5	30.5	35.0	2.0	35.0
108-109	29.2664240753871	35.0	31.0	35.0	2.0	35.0
110-111	29.175068427039378	35.0	30.5	35.0	2.0	35.0
112-113	29.06100180894302	34.0	30.0	35.0	2.0	35.0
114-115	28.968328125770093	34.0	30.0	35.0	2.0	35.0
116-117	28.856569776548934	34.0	30.0	35.0	2.0	35.0
118-119	28.707522167725664	34.0	30.0	35.0	2.0	35.0
120-121	28.845872150133978	35.0	31.0	35.0	2.0	35.0
122-123	28.755040470647252	35.0	31.0	35.0	2.0	35.0
124-125	28.062352782439344	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67357.0
3	26563.0
4	11485.0
5	10328.0
6	12103.0
7	14349.0
8	15943.0
9	17806.0
10	20309.0
11	23137.0
12	24777.0
13	29067.0
14	37227.0
15	33977.0
16	20626.0
17	17369.0
18	16657.0
19	16420.0
20	16160.0
21	16545.0
22	16626.0
23	19245.0
24	24527.0
25	32388.0
26	40197.0
27	47169.0
28	57571.0
29	69156.0
30	82459.0
31	101666.0
32	127897.0
33	165368.0
34	230125.0
35	354443.0
36	696341.0
37	1184491.0
38	399934.0
39	718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28348401836082	17.813981178750062	12.423022084883808	25.479512718005314
2	16.698368852389013	20.318908940504414	37.165763591574986	25.816958615531586
3	19.254068413863912	23.921087727636717	29.44846513112275	27.376378727376622
4	13.622155046185627	16.184965033514683	34.92900684738479	35.2638730729149
5	14.118574958169791	37.08081830833946	33.950345196953826	14.850261536536927
6	33.76624676773867	35.28381635139656	16.410584480750494	14.539352400114275
7	28.8479839071754	31.811365118158502	20.635719277423586	18.704931697242515
8	27.374446722746576	33.895132868286396	19.44451561596757	19.285904792999457
9	27.198270592532893	14.512044872062354	18.57465902693744	39.71502550846731
10-11	26.017578668524987	24.946969117463876	27.2059804024552	21.82947181155593
12-13	26.734255002814688	22.58858463455277	27.27268179758491	23.404478565047633
14-15	23.898073020208777	23.796464238906548	24.849301049200953	27.45616169168372
16-17	23.635783298805727	26.666363261340454	25.596249422116564	24.101604017737255
18-19	23.68629383179358	25.457002019894386	27.64202405277642	23.214680095535613
20-21	24.535677787980195	24.64363089481985	27.502173521776403	23.318517795423553
22-23	24.201318656430843	24.472422723687394	27.425854861886652	23.90040375799511
24-25	23.883854539224608	24.833185560914085	27.441143014171754	23.841816885689553
26-27	23.759004448305756	24.908267039890767	27.477924725954228	23.854803785849253
28-29	23.837157443504083	24.96968089253791	27.463650931805404	23.729510732152605
30-31	24.022351072471494	24.892669873727325	27.28709294142813	23.797886112373053
32-33	23.77939025796497	24.962669788855667	27.313170484087756	23.94476946909161
34-35	23.853630923756477	24.948381375880835	27.45540957445705	23.742578125905638
36-37	24.163962107660993	24.81326370972554	27.222443424514097	23.80033075809937
38-39	24.04365377099524	24.900344567602982	27.181880178229754	23.874121483172022
40-41	23.98534517294065	24.905408543450815	27.280631225534563	23.828615058073975
42-43	24.07073134571492	24.81460263189524	27.07541638525314	24.039249637136702
44-45	23.992543847606495	24.888990582183943	27.031786273235646	24.086679296973912
46-47	24.180463447869894	24.992376418355313	26.69122401591123	24.13593611786356
48-49	24.177888276484946	25.128816527853886	26.3991123986358	24.294182797025368
50-51	23.964700852573458	25.448749202649328	26.31938048964042	24.26716945513679
52-53	24.174289885763358	25.222766702042538	26.233504648847934	24.369438763346174
54-55	23.975219485792007	25.376172895980094	26.152745004565237	24.495862613662666
56-57	23.912711128095186	25.38582339758853	26.087612203789867	24.61385327052642
58-59	23.887728492342504	25.21202842427232	26.217439265452107	24.68280381793307
60-61	23.85777214229814	25.309668711398935	26.314989310240232	24.517569836062695
62-63	23.703128616536716	25.31242911816809	26.372293618033204	24.61214864726199
64-65	23.65281777046483	25.310751160996332	26.304210386434196	24.732220682104643
66-67	23.63678892980474	25.345781926007128	26.27674194741423	24.7406871967739
68-69	23.734108059400274	25.125015480896117	26.300172592926558	24.84070386677705
70-71	23.59403130630702	25.074026610775853	26.309178274724204	25.02276380819292
72-73	23.69033232425628	25.119062916546316	26.12984841146328	25.06075634773413
74-75	23.64380541366282	25.232129598956533	26.12756985484541	24.996495132535237
76-77	23.660308782469343	24.941434547059465	26.198769335505833	25.199487334965355
78-79	23.54693831236571	25.082461258398947	26.136064431665808	25.234535997569534
80-81	23.642064582347306	25.022215731068066	26.066852299033805	25.26886738755082
82-83	23.441316545732935	25.049296524773446	26.161157205796957	25.34822972369665
84-85	23.421360423998088	25.039379513087418	25.977057269952265	25.562202792962225
86-87	23.385628457149675	24.93784039240437	26.05976960896333	25.616761541482624
88-89	23.321188279250016	24.90404349767796	25.94278727771012	25.8319809453619
90-91	23.23581031977631	24.93641713289222	25.939772102214796	25.888000445116678
92-93	23.358926848861845	24.83102984372777	25.973269808314626	25.83677349909576
94-95	23.1586211389843	24.6937974484778	26.093897764151286	26.05368364838661
96-97	23.16530169713063	24.575442799094763	26.077208236160164	26.18204726761444
98-99	23.170750903162286	24.68100589954988	25.85150812574562	26.296735071542216
100-101	23.1074102587851	24.6495215481785	25.929212535307155	26.313855657729246
102-103	22.960402924588056	24.539102595467575	25.944096739189987	26.556397740754385
104-105	22.873331635752518	24.63734341860647	25.808967009058225	26.680357936582787
106-107	22.86535357372119	24.71013271696715	25.775282497439772	26.64923121187189
108-109	22.816128500148363	24.66604133806006	25.809933940312025	26.707896221479547
110-111	22.73690586533867	24.86586043823723	25.737273397732036	26.659960298692063
112-113	22.73724583548691	24.88940509605309	25.61633720025047	26.757011868209528
114-115	22.762329731007874	24.93714618420054	25.584046953624462	26.71647713116712
116-117	22.602097344699775	24.88796905580202	25.685194785419974	26.82473881407823
118-119	22.628624650244753	24.91593307636017	25.49728260272806	26.95815967066702
120-121	22.553197615298455	24.999423551947242	25.56994242473449	26.87743640801981
122-123	22.437276134339097	25.060644040133223	25.443217255542876	27.058862569984804
124-125	22.374913306856214	25.137174935022077	25.353595180684675	27.13431657743703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	151.0
2	307.0
3	565.0
4	439.0
5	286.5
6	511.5
7	1188.0
8	1944.5
9	2124.5
10	1759.0
11	1367.0
12	1380.0
13	1550.5
14	1730.5
15	1962.5
16	2233.5
17	2530.0
18	2836.5
19	3251.0
20	3673.5
21	4204.0
22	5115.0
23	6205.0
24	7382.5
25	9031.0
26	11545.0
27	14957.5
28	18299.5
29	22016.5
30	26492.0
31	32073.0
32	38615.5
33	43405.5
34	47482.5
35	52433.5
36	58485.5
37	65481.0
38	73380.0
39	81093.0
40	88874.0
41	97329.5
42	104544.0
43	113830.0
44	123459.5
45	131909.0
46	142051.0
47	151456.5
48	160878.5
49	168638.0
50	174921.5
51	179904.5
52	181048.5
53	182931.0
54	182665.0
55	175654.0
56	165535.0
57	153589.0
58	142515.5
59	129600.5
60	109671.0
61	90596.0
62	74329.0
63	57044.5
64	42434.0
65	31913.5
66	23705.5
67	17325.0
68	12579.5
69	9195.0
70	6915.0
71	5269.0
72	4134.5
73	3505.5
74	2728.0
75	2013.0
76	1471.5
77	964.0
78	601.0
79	347.5
80	232.0
81	165.0
82	106.0
83	70.0
84	54.5
85	44.5
86	29.5
87	20.5
88	16.5
89	16.0
90	13.0
91	9.0
92	8.0
93	12.5
94	17.5
95	18.5
96	18.5
97	13.5
98	5.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16513253789289126
2	0.011979916682241373
3	0.0
4	0.00422102970677751
5	0.14127030059099296
6	0.07553935244036515
7	0.007368502725126057
8	0.010857561962520183
9	0.011589532431903566
10-11	0.005038396730922288
12-13	0.009344822992461192
14-15	0.011101552118981312
16-17	0.01457841184855238
18-19	0.019202025313490752
20-21	0.02228850079272402
22-23	0.024935793990327252
24-25	0.024618606786927788
26-27	0.021922515558032325
28-29	0.02104415099477227
30-31	0.024508811216520283
32-33	0.023752441731490784
34-35	0.02258128898047737
36-37	0.023654845668906334
38-39	0.019067830727437132
40-41	0.025289579717195892
42-43	0.022166505714493454
44-45	0.03021818087771067
46-47	0.025435973811072564
48-49	0.01806747108594651
50-51	0.014151429074745408
52-53	0.015346980841404935
54-55	0.017311101600917013
56-57	0.013895239410461224
58-59	0.01340725909753897
60-61	0.016566931623710573
62-63	0.01455401283290627
64-65	0.026241141327394288
66-67	0.02001939233763553
68-69	0.01789667797642372
70-71	0.024716202849512238
72-73	0.029705801549142305
74-75	0.015944756724734697
76-77	0.00813707171797861
78-79	0.01319986746454701
80-81	0.015651968536981344
82-83	0.015139589208412976
84-85	0.014224626121683747
86-87	0.009918199860144842
88-89	0.02039757708015028
90-91	0.017664887327785646
92-93	0.01495659659106713
94-95	0.011284544736327158
96-97	0.019897397259404967
98-99	0.020507372650557788
100-101	0.02663152557773209
102-103	0.019189825805667696
104-105	0.013114470909785614
106-107	0.018189466164177073
108-109	0.010101192477490688
110-111	0.022642286519592655
112-113	0.016786522764525587
114-115	0.012809483214209206
116-117	0.010723367376466564
118-119	0.00739290174077217
120-121	0.003696450870386085
122-123	0.005501978028198431
124-125	4.5138178945308634E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4098526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.028935382160384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.61957422654409	27.579208449722987
2	14.891301959538517	12.517311356273652
3	6.829663166621821	8.611304157356082
4	3.7260929789306965	6.26414884157599
5	2.265754972521181	4.761363466595065
6	1.480833037351745	3.734270162317487
7	1.0099358306511468	2.971256943659587
8	0.7220601850456613	2.427793668745189
9	0.561073698642799	2.122319720239914
>10	2.7015972570399507	19.4789409921512
>50	0.13519697112723544	3.8916324511371045
>100	0.053718226363146125	3.906098981444534
>500	0.0022673108582338527	0.6738168447074389
>1k	7.557702454918304E-4	0.4824251817038112
>5k	1.7440851819041812E-4	0.5781087823699342
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9567	0.2334253826863609	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8515	0.20775761822665026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5578	0.13609770927401704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.879803129222555E-5
2	0.0	0.0	0.0	0.0	4.879803129222555E-5
3	0.0	0.0	0.0	0.0	4.879803129222555E-5
4	0.0	0.0	0.0	0.0	4.879803129222555E-5
5	0.0	0.0	0.0	0.0	4.879803129222555E-5
6	0.0	0.0	0.0	0.0	1.7079310952278941E-4
7	0.0	0.0	0.0	0.0	1.951921251689022E-4
8	0.0	0.0	0.0	0.0	1.951921251689022E-4
9	0.0	0.0	0.0	2.4399015646112774E-5	2.683891721072405E-4
10-11	0.0	0.0	0.0	4.879803129222555E-5	2.683891721072405E-4
12-13	0.0	0.0	0.0	1.0979557040750748E-4	4.879803129222555E-4
14-15	0.0	0.0	0.0	1.4639409387667664E-4	6.953719459142141E-4
16-17	0.0	0.0	0.0	1.7079310952278941E-4	8.173670241447779E-4
18-19	0.0	0.0	0.0	2.3179064863807137E-4	8.539655476139471E-4
20-21	0.0	0.0	0.0	2.805886799302969E-4	9.515616101983982E-4
22-23	0.0	0.0	0.0	4.3918228163002994E-4	0.0011223547197211875
24-25	0.0	0.0	0.0	8.051675163217216E-4	0.0012565493057748079
26-27	0.0	0.0	0.0	0.0014883399544128792	0.0013663448761823153
28-29	0.0	0.0	0.0	0.004611413957115314	0.001415142907474541
30-31	0.0	0.0	0.0	0.015176187731882144	0.0014395419231206536
32-33	0.0	0.0	0.0	0.04096594726982335	0.0014639409387667663
34-35	0.0	0.0	0.0	0.08140731570325527	0.001512738970058992
36-37	0.0	0.0	0.0	0.1470528672991217	0.0015615370013512175
38-39	0.0	0.0	0.0	0.25232242030427526	0.0015981355248203867
40-41	0.0	0.0	0.0	0.37640361437258174	0.0016591330639356685
42-43	0.0	0.0	0.0	0.4953854141708507	0.0016591330639356685
44-45	0.0	0.0	0.0	0.6386442345370018	0.001671332571758725
46-47	0.0	0.0	0.0	0.8144513417750674	0.001793327649989289
48-49	0.0	0.0	0.0	1.0033851194307417	0.001927522236042909
50-51	0.0	0.0	0.0	1.2062507350203462	0.001927522236042909
52-53	0.0	0.0	0.0	1.4093969392898813	0.002049517314273473
54-55	0.0	0.0	0.0	1.637966917862666	0.0022813079629115445
56-57	0.0	0.0	0.0	1.9053191318049465	0.002366704517672939
58-59	0.0	0.0	0.0	2.2049268444313883	0.002403303041142108
60-61	0.0	0.0	0.0	2.5267864593270852	0.0024643005802573903
62-63	0.0	0.0	0.0	2.8588326632550336	0.0026350936897801794
64-65	0.0	0.0	0.0	3.203968939077122	0.0026350936897801794
66-67	0.0	0.0	0.0	3.6082972268566795	0.002647293197603236
68-69	0.0	0.0	0.0	4.066108156932517	0.0026594927054262923
70-71	0.0	0.0	0.0	4.571497167518274	0.002708290736718518
72-73	0.0	0.0	0.0	5.083290919711136	0.0030132784322949276
74-75	0.0	0.0	0.0	5.5983175414771065	0.003098674987056322
76-77	0.0	0.0	0.0	6.170206069206344	0.003147473018348548
78-79	0.0	0.0	0.0	6.8140472940759675	0.0032084705574638296
80-81	0.0	0.0	0.0	7.497488121339233	0.003281667604402168
82-83	0.0	0.0	0.0	8.214989974444471	0.00347685972957107
84-85	0.0	0.0	0.0	8.930625302852782	0.0035012587452171832
86-87	0.0	0.0	0.0	9.67550285151296	0.003586655299978578
88-89	0.0	0.0	0.0	10.483415257094867	0.0036842513625630286
90-91	0.0	0.0	0.0	11.350007295305678	0.0036842513625630286
92-93	0.0	0.0	0.0	12.236789518963647	0.0037330493938552544
94-95	0.0	0.0	0.0	13.134624496709305	0.003903842503378044
96-97	0.0	0.0	0.0	14.035875336645418	0.00408683512072389
98-99	0.0	0.0	0.0	14.971443392087789	0.004208830198954454
100-101	0.0	0.0	0.0	15.978110179122933	0.00422102970677751
102-103	0.0	0.0	0.0	17.007870634467125	0.004343024785008073
104-105	0.0	0.0	0.0	18.031543535407607	0.0044650198632386374
106-107	0.0	0.0	0.0	19.048762896709697	0.004513817894530863
108-109	0.0	0.0	0.0	20.08561126609908	0.0045260174023539195
110-111	0.0	0.0	0.0	21.164035558149443	0.004562615925823089
112-113	0.0	0.0	0.0	22.281876459976097	0.0046358129727614265
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9205	0.0	47.63058	1
GTATCAA	14450	0.0	43.42804	1
TCAACGC	18270	0.0	34.054565	4
ATCAACG	18605	0.0	33.60154	3
TATCAAC	18790	0.0	33.334145	2
CAACGCA	18775	0.0	33.138584	5
AACGCAG	19320	0.0	32.320866	6
GTACATG	17235	0.0	29.211475	1
ACGCAGA	21605	0.0	28.773348	7
TACATGG	17590	0.0	28.22231	2
ACATGGG	17595	0.0	27.638487	3
CATGGGG	12465	0.0	27.58652	4
CGCAGAG	22495	0.0	27.423391	8
GCAGAGT	24310	0.0	24.886227	9
GAGTACT	12840	0.0	24.091658	12-13
AGTACTT	13615	0.0	24.052933	12-13
AGAGTAC	21080	0.0	23.858889	10-11
GTACTTT	13765	0.0	22.69032	14-15
CAGAGTA	23155	0.0	21.87495	10-11
TACTTTT	15240	0.0	21.177383	14-15
>>END_MODULE