FastQCFastQC Report
Mon 15 Aug 2016
SRR2921078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370179
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80250.041.7719761
GGTATCA51750.041.1482731
TCAACGC91500.035.847794
CAACGCA95450.034.3643045
AACGCAG96050.034.0825846
TATCAAC97350.034.0601162
ATCAACG97350.033.877063
GTACATG153750.032.1224751
TACATGG159950.030.4808162
ACATGGG156650.030.1732443
CATGGGG118050.029.701694
ACGCAGA110350.029.178127
CGCAGAG114450.028.0808568
GCAGAGT131300.023.5703499
AGAGTAC115050.020.71517410-11
CAGAGTA125400.020.26296610-11
ATGGGGG62650.019.9540065
ATGGGGA66000.019.3019755
GAGTACA113650.018.8941271
GAGTACT48850.016.93237712-13
Produced by FastQC (version 0.11.5)