##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921078_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4370179
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91406484722937	31.0	31.0	34.0	25.0	34.0
2	29.939883698127698	31.0	30.0	34.0	25.0	34.0
3	30.107286452111	31.0	31.0	34.0	26.0	34.0
4	33.49294319523296	37.0	35.0	37.0	27.0	37.0
5	33.58542064295307	37.0	35.0	37.0	28.0	37.0
6	33.52751203097173	37.0	35.0	37.0	28.0	37.0
7	33.43960281718438	37.0	35.0	37.0	27.0	37.0
8	33.4382735810135	37.0	35.0	37.0	27.0	37.0
9	34.880692301162036	39.0	35.0	39.0	27.0	39.0
10-11	34.83198159617719	39.0	35.0	39.0	27.0	39.0
12-13	34.74804842089992	39.0	35.0	39.0	27.0	39.0
14-15	35.95117842999109	40.0	36.0	41.0	27.0	41.0
16-17	35.90967280745251	40.0	36.0	41.0	27.0	41.0
18-19	35.926599574067794	40.0	36.0	41.0	27.0	41.0
20-21	35.97372647665004	40.0	36.0	41.0	27.0	41.0
22-23	35.89628674248812	40.0	36.0	41.0	27.0	41.0
24-25	35.87679417708062	40.0	36.0	41.0	27.0	41.0
26-27	35.823012741583355	40.0	36.0	41.0	27.0	41.0
28-29	35.6956928995357	40.0	36.0	41.0	26.0	41.0
30-31	35.59274826042595	40.0	35.5	41.0	26.0	41.0
32-33	35.48148645627559	40.0	35.0	41.0	25.0	41.0
34-35	35.373744416418646	40.0	35.0	41.0	25.0	41.0
36-37	35.24396369118976	40.0	35.0	41.0	24.5	41.0
38-39	35.10142536495644	40.0	35.0	41.0	24.0	41.0
40-41	34.96379724949482	40.0	35.0	41.0	24.0	41.0
42-43	34.755630490192736	39.0	34.5	41.0	23.0	41.0
44-45	34.545615522842425	39.0	34.0	41.0	22.0	41.0
46-47	34.35772127411715	39.0	34.0	41.0	21.5	41.0
48-49	34.116753341224694	39.0	33.5	41.0	19.5	41.0
50-51	33.9269484156141	38.0	33.0	40.0	18.5	41.0
52-53	33.73469164535366	38.0	33.0	40.0	17.5	41.0
54-55	33.493552323600476	38.0	33.0	40.0	14.5	41.0
56-57	33.23641560219845	38.0	33.0	40.0	13.0	41.0
58-59	32.94894568391821	37.0	32.0	40.0	10.5	41.0
60-61	32.63855725360449	37.0	32.0	40.0	8.0	41.0
62-63	32.34276387763522	36.0	31.5	39.5	7.5	41.0
64-65	32.009025030782496	36.0	31.0	39.0	6.5	41.0
66-67	31.65907048658648	35.0	31.0	39.0	2.0	40.5
68-69	31.295169030833748	35.0	31.0	38.5	2.0	40.0
70-71	30.928175024409754	35.0	30.5	37.5	2.0	40.0
72-73	30.575117518069625	35.0	30.0	37.0	2.0	39.0
74-75	30.527111932943708	35.0	30.0	36.5	2.0	39.0
76-77	30.581327446770487	35.0	31.0	36.0	2.0	39.0
78-79	30.40670622416153	35.0	31.0	36.0	2.0	38.0
80-81	30.20057153265347	35.0	31.0	35.5	2.0	37.0
82-83	29.963993351302086	35.0	31.0	35.0	2.0	37.0
84-85	29.71968676797907	35.0	31.0	35.0	2.0	36.0
86-87	29.57961264286886	35.0	31.0	35.0	2.0	36.0
88-89	29.41199296413259	35.0	30.5	35.0	2.0	36.0
90-91	29.275817420750958	35.0	30.0	35.0	2.0	35.5
92-93	29.167829281134708	35.0	30.0	35.0	2.0	35.0
94-95	29.055688451205317	35.0	30.0	35.0	2.0	35.0
96-97	28.95745506076525	35.0	30.0	35.0	2.0	35.0
98-99	28.857970806230135	34.0	30.0	35.0	2.0	35.0
100-101	28.72847794106374	34.0	30.0	35.0	2.0	35.0
102-103	27.415409414580044	32.5	26.0	34.5	2.0	35.0
104-105	28.379580104155917	34.0	29.0	35.0	2.0	35.0
106-107	28.522819889070906	34.0	29.0	35.0	2.0	35.0
108-109	28.46402893336863	34.0	29.0	35.0	2.0	35.0
110-111	28.365250485163195	34.0	29.0	35.0	2.0	35.0
112-113	28.249920884247533	34.0	29.0	35.0	2.0	35.0
114-115	28.106394040152587	34.0	29.0	35.0	2.0	35.0
116-117	27.965257944811874	34.0	29.0	35.0	2.0	35.0
118-119	27.757056289913983	34.0	29.0	35.0	2.0	35.0
120-121	27.555014451353138	34.0	29.0	35.0	2.0	35.0
122-123	27.256001413214424	34.0	27.5	35.0	2.0	35.0
124-125	25.939373993605297	33.0	23.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164460.0
3	24955.0
4	16157.0
5	15169.0
6	19044.0
7	20743.0
8	20725.0
9	22108.0
10	23846.0
11	25279.0
12	26389.0
13	26116.0
14	25920.0
15	23089.0
16	19172.0
17	17302.0
18	16305.0
19	16742.0
20	17131.0
21	18635.0
22	21716.0
23	27905.0
24	36793.0
25	46119.0
26	52337.0
27	58502.0
28	67979.0
29	80131.0
30	95380.0
31	115228.0
32	145157.0
33	187649.0
34	259271.0
35	396441.0
36	746068.0
37	1139157.0
38	334785.0
39	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33873110456877	17.322880181090465	11.504839474747689	25.83354923959307
2	16.714661705958026	20.011664443232355	37.18047004912622	26.0932038016834
3	19.180672764442217	23.786019237740653	28.93186181235428	28.101446185462848
4	13.135703556250444	15.87694988252554	35.15027318337123	35.83707337785278
5	14.224979747246552	37.34738287701557	33.866840980441694	14.560796395296185
6	34.00473249576834	35.236106999859786	16.60302755093344	14.156132953438435
7	29.112931159294124	31.948809770347715	20.664948840925792	18.273310229432365
8	27.216103747942533	34.28220274237462	19.507673769442142	18.9940197402407
9	26.88131741246117	15.345642073623342	18.507968072725273	39.26507244119022
10-11	25.651077202520256	25.60467128473501	27.232096586611114	21.51215492613362
12-13	26.098593641193734	23.262492254554253	26.907337844348117	23.731576259903893
14-15	23.524239276192755	24.580583635498034	24.050871094425073	27.844305993884134
16-17	23.86613188564824	27.1380942831564	25.065735758912883	23.93003807228248
18-19	24.00034401296622	26.347671367757304	26.29444326299734	23.357541356279135
20-21	24.795007483735613	25.701587330671178	25.90600153224763	23.597403653345577
22-23	24.48286624835381	25.52366011000463	25.767516917580334	24.225956724061216
24-25	24.171462191825434	25.668027895768525	25.97733325668738	24.183176655718665
26-27	23.962701720269774	25.81078063210552	25.91756310576206	24.308954541862647
28-29	24.021295951474308	26.124094231527685	25.85526668379834	23.99934313319967
30-31	24.174704106150884	25.965875459776427	25.64973318338485	24.20968725068784
32-33	23.862538519292514	26.001329823174284	25.651386602913334	24.484745054619864
34-35	24.204537191260915	25.995709130093974	25.77992887485192	24.01982480379319
36-37	24.41261474072644	25.783182254274084	25.600577336606616	24.20362566839286
38-39	24.079084425414564	25.95064626025989	25.70784279801191	24.262426516313635
40-41	24.244653138216567	25.86207567851815	25.827812521901166	24.065458661364122
42-43	24.14011924925549	25.876083095240933	25.65029226052362	24.33350539497995
44-45	24.016594249262734	25.935029520378016	25.86569979852588	24.182676431833368
46-47	24.140832331558762	25.92879958784132	25.709725799988554	24.22064228061137
48-49	23.950535924928033	26.057839064867988	25.551298157765572	24.440326852438403
50-51	23.982145447555116	26.14083759448994	25.61570235207143	24.261314605883516
52-53	24.22369465932064	25.929052400274134	25.52843703785555	24.31881590254968
54-55	23.877460204328422	25.971886061987437	25.672971923164305	24.477681810519837
56-57	23.845385050304415	26.103864469017584	25.57453174478927	24.47621873588873
58-59	23.877752403313618	25.728870892825846	25.71918516714689	24.674191536713654
60-61	23.689125903777665	25.80011418670144	25.93570230661259	24.575057602908306
62-63	23.716758726397945	25.838634855434634	25.940254599026286	24.504351819141135
64-65	23.649967532419204	25.781526330114513	25.839488204209005	24.729017933257282
66-67	23.615691010664754	25.844991467647134	25.80080688670336	24.738510634984753
68-69	23.891694459969457	25.60715584940673	25.84861320894392	24.652536481679892
70-71	23.619701342211563	25.585997368913123	25.8903048147012	24.903996474174114
72-73	23.64892101090237	25.605145068242997	25.941829369554668	24.804104551299964
74-75	23.506762691426367	25.804268437223378	25.914202968919447	24.77476590243081
76-77	23.536755176322078	25.486774856543953	26.109762386372054	24.866707580761915
78-79	23.54718999760355	25.621671889510882	26.088229943001558	24.742908169884014
80-81	23.61096097224029	25.52761725642233	26.080507465311815	24.78091430602557
82-83	23.49431600857695	25.508551982743494	26.17701704166764	24.820114967011918
84-85	23.36828960564052	25.544571508998498	26.123330657720828	24.96380822764015
86-87	23.351744803195068	25.60441692062785	26.160322236601967	24.883516039575117
88-89	23.422474941551947	25.370640409338453	26.064403740538143	25.14248090857145
90-91	23.323923010866512	25.436521891682084	26.11032968497731	25.129225412474092
92-93	23.461669372557356	25.355346358015385	26.21463695098382	24.968347318443435
94-95	23.268233519229735	25.17172198839639	26.36963323337093	25.190411259002943
96-97	23.359403939045052	25.070296742309782	26.32125070205139	25.24904861659378
98-99	23.18203806931801	25.24423203861318	26.186662056228645	25.387067835840167
100-101	23.243712323670398	25.246795311138158	26.24935599321106	25.260136371980384
102-103	23.125888623930745	25.207170516077632	26.340350278251844	25.326590581739776
104-105	22.949954220102782	25.360105232510012	26.169637160109883	25.52030338727732
106-107	22.978286841617376	25.417265426650914	26.11865771037388	25.485790021357822
108-109	22.98985787372422	25.438377094886977	26.161766009390185	25.40999902199862
110-111	23.029221545860107	25.623377786348406	26.067308654653687	25.2800920131378
112-113	23.095021171679356	25.685579178622888	26.02688169519079	25.192517954506965
114-115	23.073240099838717	25.84594486517238	25.909821306285817	25.170993728703085
116-117	22.94556786711531	25.793734902785243	26.01330472913901	25.24739250096044
118-119	23.084399874234084	25.791641696300964	25.870451490928343	25.253506938536603
120-121	23.095610964816682	25.965908785045617	25.85210570629208	25.086374543845626
122-123	23.201557274307334	25.9498615203968	25.736055792175932	25.112525413119936
124-125	23.268351375605107	26.042228250629805	25.605823464631595	25.083596909133494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1684.0
1	1376.0
2	969.0
3	764.5
4	601.0
5	510.0
6	478.5
7	512.0
8	596.5
9	659.0
10	655.0
11	666.0
12	741.0
13	832.0
14	963.0
15	1139.5
16	1326.0
17	1543.0
18	1820.0
19	2124.0
20	2576.5
21	3237.5
22	4050.0
23	5161.5
24	6668.0
25	8698.5
26	11674.0
27	15370.5
28	18599.0
29	22590.0
30	27466.5
31	33431.5
32	42525.0
33	48031.0
34	51349.5
35	57571.5
36	64711.5
37	73484.5
38	82382.5
39	90502.0
40	99394.0
41	108817.5
42	117673.5
43	128558.0
44	140103.5
45	152725.5
46	165081.5
47	172730.5
48	178166.0
49	184012.0
50	189013.0
51	191686.5
52	191430.5
53	190626.0
54	188170.5
55	178512.5
56	166371.0
57	153711.0
58	140669.0
59	125909.0
60	107115.0
61	89336.0
62	74141.5
63	57579.5
64	42889.0
65	33403.0
66	25755.5
67	19803.5
68	15309.5
69	11848.5
70	9413.5
71	7215.5
72	5505.0
73	4642.0
74	3794.0
75	2900.0
76	2039.5
77	1370.5
78	963.0
79	680.0
80	481.5
81	341.0
82	244.0
83	169.0
84	119.0
85	102.0
86	78.0
87	61.5
88	50.0
89	37.0
90	25.5
91	18.5
92	21.5
93	19.5
94	19.0
95	18.0
96	13.5
97	15.0
98	21.0
99	21.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28781429776675055
2	0.07004289755637011
3	0.07894413478257985
4	0.08461895954376239
5	0.09395496157022401
6	0.1250063212513721
7	0.11374820116063897
8	0.11404567181344288
9	0.12033832023814128
10-11	0.09968699222617655
12-13	0.0893212840938552
14-15	0.09886322734148877
16-17	0.10078534540576027
18-19	0.09300534371704225
20-21	0.09253625537992838
22-23	0.07487107507495688
24-25	0.08654107760803391
26-27	0.0982568448569269
28-29	0.09146078455825266
30-31	0.08673557765025185
32-33	0.09576266784495555
34-35	0.08996199011527903
36-37	0.0897446077151531
38-39	0.07959628198295768
40-41	0.09091160796846079
42-43	0.08070607634149539
44-45	0.07699913436040033
46-47	0.0670224263125149
48-49	0.06886445612410842
50-51	0.07124422134653981
52-53	0.0834405181115007
54-55	0.08866913689347736
56-57	0.0963461679716094
58-59	0.0669881027756529
60-61	0.0834290769325467
62-63	0.08927551937803921
64-65	0.08044292922555346
66-67	0.07484819271704889
68-69	0.08167857655258515
70-71	0.0810607528890693
72-73	0.06257180769941002
74-75	0.09024801958912897
76-77	0.09428675575988993
78-79	0.07563763406487468
80-81	0.0881199603036855
82-83	0.0928337260327323
84-85	0.09611734439252946
86-87	0.08461895954376239
88-89	0.06497445527974942
90-91	0.08865769571452337
92-93	0.06534057300627731
94-95	0.0923875200535264
96-97	0.08035139979392149
98-99	0.07497404568554285
100-101	0.09060269613670287
102-103	0.0645854551953135
104-105	0.07064928004093196
106-107	0.08840598977753543
108-109	0.09454990287583186
110-111	0.08800554851414552
112-113	0.07730804619215825
114-115	0.090087843083773
116-117	0.08435581242782046
118-119	0.07633554598106851
120-121	0.1085424647365703
122-123	0.08768519550343361
124-125	0.10838228823121432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4370179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.057457193084915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.06492164821017	34.15199430038795
2	13.705205336096812	13.172746375238232
3	5.772568585584756	8.322449030876584
4	2.9838478836995384	5.735845677662703
5	1.6817207355602728	4.040961112995554
6	1.0650318135794727	3.070963247422146
7	0.7095878082078912	2.3870690002379975
8	0.5035597655467807	1.9359841501539434
9	0.3770691973511121	1.6308888129478762
>10	1.9803656842081931	16.807214107065697
>50	0.10873283311737911	3.5675498265685066
>100	0.043904518018876126	3.5122657248600966
>500	0.0021000606027511593	0.6279051401831361
>1k	0.0013841302160706856	1.036163493399621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.152943163197663E-5
2	0.0	0.0	0.0	0.0	9.152943163197663E-5
3	0.0	0.0	0.0	0.0	9.152943163197663E-5
4	0.0	0.0	0.0	0.0	9.152943163197663E-5
5	0.0	0.0	0.0	0.0	1.8305886326395326E-4
6	0.0	0.0	0.0	0.0	2.9747065280392407E-4
7	0.0	0.0	0.0	0.0	2.9747065280392407E-4
8	0.0	0.0	0.0	0.0	2.9747065280392407E-4
9	0.0	0.0	0.0	0.0	2.9747065280392407E-4
10-11	0.0	0.0	0.0	0.0	3.432353686199124E-4
12-13	0.0	0.0	0.0	1.1441178953997079E-5	7.32235453055813E-4
14-15	0.0	0.0	0.0	4.5764715815988316E-5	9.381766742277604E-4
16-17	0.0	0.0	0.0	5.720589476998539E-5	0.001144117895399708
18-19	0.0	0.0	0.0	8.008825267797956E-5	0.0012127649691236904
20-21	0.0	0.0	0.0	9.152943163197663E-5	0.0013958238323876437
22-23	0.0	0.0	0.0	1.1441178953997078E-4	0.0014759120850656232
24-25	0.0	0.0	0.0	3.661177265279065E-4	0.0014873532640196203
26-27	0.0	0.0	0.0	8.466472425957838E-4	0.0016475297693755793
28-29	0.0	0.0	0.0	0.0030776771386252138	0.0017047356641455647
30-31	0.0	0.0	0.0	0.013042944007556668	0.0017276180220535588
32-33	0.0	0.0	0.0	0.03705797863199654	0.0017962650957775413
34-35	0.0	0.0	0.0	0.07780001688718013	0.0019335592432255064
36-37	0.0	0.0	0.0	0.14634412000057664	0.0019907651379954916
38-39	0.0	0.0	0.0	0.26211740983607307	0.0021051769275354623
40-41	0.0	0.0	0.0	0.3979013216621104	0.0021051769275354623
42-43	0.0	0.0	0.0	0.5336394687723318	0.0021051769275354623
44-45	0.0	0.0	0.0	0.7021451524068008	0.002208147538121436
46-47	0.0	0.0	0.0	0.9053404906297888	0.00231111814870741
48-49	0.0	0.0	0.0	1.124072949872305	0.0024026475803393867
50-51	0.0	0.0	0.0	1.3635711489163258	0.0024941770119713633
52-53	0.0	0.0	0.0	1.6045452600454122	0.002768765306867293
54-55	0.0	0.0	0.0	1.8753007599917533	0.002871735917453267
56-57	0.0	0.0	0.0	2.2072436849840704	0.0029403829911772493
58-59	0.0	0.0	0.0	2.5684302633828042	0.0029747065280392405
60-61	0.0	0.0	0.0	2.9597300247884584	0.0029975888859472347
62-63	0.0	0.0	0.0	3.3588898761355086	0.0031577653913031937
64-65	0.0	0.0	0.0	3.7851195568877154	0.003203530107119182
66-67	0.0	0.0	0.0	4.295384697057031	0.0032836183597971615
68-69	0.0	0.0	0.0	4.863038790859596	0.0033179418966591528
70-71	0.0	0.0	0.0	5.484409219851178	0.0033865889703831352
72-73	0.0	0.0	0.0	6.112770209183651	0.0035810890126010855
74-75	0.0	0.0	0.0	6.760054908506037	0.0037527066969110416
76-77	0.0	0.0	0.0	7.489327096212763	0.0038213537706350245
78-79	0.0	0.0	0.0	8.308687127003264	0.003912883202267001
80-81	0.0	0.0	0.0	9.168171372385434	0.003970089097036986
82-83	0.0	0.0	0.0	10.05604118275247	0.004233236212978919
84-85	0.0	0.0	0.0	10.947892523395495	0.004290442107748904
86-87	0.0	0.0	0.0	11.87995960806182	0.004404853897288875
88-89	0.0	0.0	2.2882357907994158E-5	12.885444738075947	0.004496383328920852
90-91	0.0	0.0	2.2882357907994158E-5	13.955698382148649	0.004507824507874849
92-93	0.0	0.0	2.2882357907994158E-5	15.036798263869741	0.0046680010132308086
94-95	0.0	0.0	2.2882357907994158E-5	16.11133090887124	0.004690883371138803
96-97	0.0	0.0	2.2882357907994158E-5	17.209066264791442	0.0050913246345287
98-99	0.0	0.0	2.2882357907994158E-5	18.362634574007153	0.005354471750470633
100-101	0.0	1.1441178953997079E-5	2.2882357907994158E-5	19.594586400236693	0.005354471750470633
102-103	0.0	2.2882357907994158E-5	2.2882357907994158E-5	20.82277407859037	0.005388795287332625
104-105	0.0	2.2882357907994158E-5	2.2882357907994158E-5	22.02925784046832	0.0054231188241946155
106-107	0.0	2.2882357907994158E-5	2.2882357907994158E-5	23.23475766095622	0.0054231188241946155
108-109	0.0	2.2882357907994158E-5	2.2882357907994158E-5	24.473803018137247	0.0054231188241946155
110-111	0.0	2.2882357907994158E-5	2.2882357907994158E-5	25.75132506014056	0.005491765897918598
112-113	0.0	4.5764715815988316E-5	2.2882357907994158E-5	27.045928782322186	0.005537530613734586
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8025	0.0	41.771976	1
GGTATCA	5175	0.0	41.148273	1
TCAACGC	9150	0.0	35.84779	4
CAACGCA	9545	0.0	34.364304	5
AACGCAG	9605	0.0	34.082584	6
TATCAAC	9735	0.0	34.060116	2
ATCAACG	9735	0.0	33.87706	3
GTACATG	15375	0.0	32.122475	1
TACATGG	15995	0.0	30.480816	2
ACATGGG	15665	0.0	30.173244	3
CATGGGG	11805	0.0	29.70169	4
ACGCAGA	11035	0.0	29.17812	7
CGCAGAG	11445	0.0	28.080856	8
GCAGAGT	13130	0.0	23.570349	9
AGAGTAC	11505	0.0	20.715174	10-11
CAGAGTA	12540	0.0	20.262966	10-11
ATGGGGG	6265	0.0	19.954006	5
ATGGGGA	6600	0.0	19.301975	5
GAGTACA	11365	0.0	18.894127	1
GAGTACT	4885	0.0	16.932377	12-13
>>END_MODULE