FastQCFastQC Report
Mon 15 Aug 2016
SRR2921078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370179
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76780.17569074401757914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62760.14360967823057133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69250.053.2350041
GTATCAA114600.047.1598241
TCAACGC143950.036.928224
TATCAAC146450.036.4211582
ATCAACG148750.035.7365843
CAACGCA149600.035.653045
AACGCAG155400.034.3205766
GTACATG157650.031.4816861
ACGCAGA176350.029.9875187
ACATGGG158600.029.8347573
TACATGG162950.029.7707752
CGCAGAG182000.029.0565878
CATGGGG112950.028.544094
GCAGAGT198900.025.7793399
GAGTACT115550.023.55335412-13
AGAGTAC185500.023.07244710-11
CAGAGTA197350.021.86789910-11
GTACTTT125200.021.6917914-15
AGTACTT128600.021.27886612-13
ATGGGGA61850.019.6560215
Produced by FastQC (version 0.11.5)