##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4370179
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294530269812746	31.0	31.0	34.0	28.0	34.0
2	31.40469875490226	33.0	31.0	34.0	28.0	34.0
3	31.493096964678106	34.0	31.0	34.0	28.0	34.0
4	34.9684941966908	37.0	35.0	37.0	32.0	37.0
5	34.72276741982422	37.0	35.0	37.0	32.0	37.0
6	34.49067944356513	37.0	35.0	37.0	31.0	37.0
7	34.43014347924879	37.0	35.0	37.0	30.0	37.0
8	34.42034227888606	37.0	35.0	37.0	30.0	37.0
9	35.986270585255205	39.0	37.0	39.0	30.0	39.0
10-11	35.94803919930969	39.0	37.0	39.0	30.0	39.0
12-13	35.898080032877374	39.0	37.0	39.0	30.0	39.0
14-15	37.1668263473876	40.0	37.0	41.0	31.0	41.0
16-17	37.15551330963789	40.0	37.0	41.0	31.0	41.0
18-19	37.164252883005474	40.0	37.5	41.0	31.0	41.0
20-21	37.16257457646472	40.0	37.0	41.0	31.0	41.0
22-23	37.12761982975984	40.0	37.0	41.0	30.5	41.0
24-25	37.08436507978277	40.0	37.0	41.0	30.0	41.0
26-27	36.973248006546186	40.0	37.0	41.0	30.0	41.0
28-29	36.84934278435735	40.0	37.0	41.0	30.0	41.0
30-31	36.727427984071134	40.0	37.0	41.0	30.0	41.0
32-33	36.61481040021473	40.0	36.0	41.0	30.0	41.0
34-35	36.47967142764632	40.0	36.0	41.0	30.0	41.0
36-37	36.31089092689338	40.0	36.0	41.0	29.0	41.0
38-39	36.16764622227144	40.0	36.0	41.0	28.0	41.0
40-41	36.03493323728845	40.0	35.0	41.0	28.0	41.0
42-43	35.88285662898476	40.0	35.0	41.0	27.0	41.0
44-45	35.71702463446005	40.0	35.0	41.0	27.0	41.0
46-47	35.470401212398855	40.0	35.0	41.0	26.0	41.0
48-49	35.22789672917288	39.5	35.0	41.0	24.5	41.0
50-51	35.028387624397084	39.0	35.0	41.0	24.0	41.0
52-53	34.81237313162687	39.0	35.0	41.0	24.0	41.0
54-55	34.59510171093679	39.0	34.0	41.0	23.0	41.0
56-57	34.34174195153105	38.0	34.0	41.0	22.5	41.0
58-59	34.07931379927459	38.0	34.0	40.0	22.0	41.0
60-61	33.78242001071352	37.0	34.0	40.0	21.5	41.0
62-63	33.485054731167764	37.0	33.0	40.0	20.5	41.0
64-65	33.163768463488566	36.0	33.0	39.5	20.0	41.0
66-67	32.829766240696316	36.0	33.0	39.0	19.0	41.0
68-69	32.479256799321035	35.0	33.0	39.0	18.0	41.0
70-71	32.095456845131515	35.0	32.0	38.0	16.0	40.0
72-73	31.725997264642935	35.0	32.0	37.0	13.0	39.5
74-75	31.410300356118135	35.0	32.0	37.0	12.0	39.0
76-77	31.118800053727774	35.0	32.0	36.0	9.5	39.0
78-79	30.828849230203154	35.0	31.0	36.0	7.5	37.5
80-81	30.802636230689863	35.0	32.0	35.5	7.5	37.0
82-83	30.619409525330653	35.0	32.0	35.0	7.0	37.0
84-85	30.433333051117586	35.0	32.0	35.0	6.0	36.0
86-87	30.276685691821775	35.0	32.0	35.0	2.0	36.0
88-89	30.11065599372474	35.0	31.0	35.0	2.0	36.0
90-91	29.96651155478986	35.0	31.0	35.0	2.0	35.5
92-93	29.869246774560036	35.0	31.0	35.0	2.0	35.0
94-95	29.769512759088357	35.0	31.0	35.0	2.0	35.0
96-97	29.70418683079114	35.0	31.0	35.0	2.0	35.0
98-99	29.613972677091716	35.0	31.0	35.0	2.0	35.0
100-101	29.520946739252558	35.0	31.0	35.0	2.0	35.0
102-103	28.192506874432375	33.0	28.0	34.5	2.0	35.0
104-105	29.21002434911705	34.0	30.0	35.0	2.0	35.0
106-107	29.377326649549136	35.0	31.0	35.0	2.0	35.0
108-109	29.373713639647256	35.0	31.0	35.0	2.0	35.0
110-111	29.275629213357163	35.0	31.0	35.0	2.0	35.0
112-113	29.1579578319332	35.0	30.0	35.0	2.0	35.0
114-115	29.06352211202333	34.5	30.0	35.0	2.0	35.0
116-117	28.94937072371635	34.0	30.0	35.0	2.0	35.0
118-119	28.791523642395425	34.0	30.0	35.0	2.0	35.0
120-121	28.916593462189994	35.0	31.0	35.0	2.0	35.0
122-123	28.817535620394494	35.0	31.0	35.0	2.0	35.0
124-125	28.114211683320065	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78013.0
3	29473.0
4	13027.0
5	11594.0
6	13022.0
7	15362.0
8	16952.0
9	18501.0
10	21028.0
11	23317.0
12	25146.0
13	28767.0
14	34638.0
15	32333.0
16	20794.0
17	18156.0
18	16939.0
19	16498.0
20	16886.0
21	16932.0
22	17824.0
23	20149.0
24	26142.0
25	33864.0
26	41973.0
27	50632.0
28	60490.0
29	73214.0
30	87952.0
31	107485.0
32	134926.0
33	174188.0
34	240704.0
35	375004.0
36	736767.0
37	1280418.0
38	440375.0
39	694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.310243656451284	17.690581520617965	12.088500240676645	25.910674582254106
2	16.877863666312173	20.293037124306316	36.6285191585441	26.20058005083741
3	19.207908875128457	23.73371891631899	29.418817856202228	27.639554352350327
4	13.438360270847301	16.046215208730455	35.005183078222146	35.5102414422001
5	14.139252694955468	37.10903610399336	34.05275196876918	14.698959232281993
6	34.070740324467316	35.37284154852492	16.309058282655354	14.247359844352417
7	29.074154041973	31.67975991628009	20.734819302170873	18.511266739576033
8	27.60772223109663	33.664178424750176	19.515069738361568	19.21302960579163
9	27.145590958648047	14.410387009189831	18.703125128729965	39.74089690343216
10-11	25.940488817339393	25.00595546615682	27.3474777084785	21.70607800802529
12-13	26.569676900385726	22.5787095467543	27.26166869388208	23.589944858977894
14-15	23.783492649275807	23.89268850072597	24.6610643385893	27.66275451140892
16-17	23.74414650984201	26.66371972957599	25.603623382355245	23.98851037822675
18-19	23.80899196305882	25.525310093735325	27.416746846880663	23.2489510963252
20-21	24.537050600115354	24.90628633421527	27.137149932710177	23.419513132959196
22-23	24.203037041190832	24.77691400085373	27.05802513358152	23.962023824373922
24-25	23.902736068772683	25.00994206878115	27.236347493929188	23.850974368516983
26-27	23.70262722307364	25.17084457115026	27.155795509267648	23.97073269650845
28-29	23.77375194027678	25.377801814602236	27.122013942070232	23.72643230305075
30-31	23.952446325133597	25.250739056516192	26.925838459503183	23.870976158847025
32-33	23.70240468233625	25.242160801501267	26.88291023714032	24.172524279022163
34-35	23.951198522360286	25.254936337440174	27.055889845346787	23.73797529485275
36-37	24.196836877160056	25.03380558925179	26.814664347348423	23.95469318623973
38-39	23.95334019327457	25.210272609426436	26.84852224415288	23.98786495314611
40-41	24.001766534741144	25.10248613786364	26.998638477337693	23.897108850057524
42-43	24.066391845921554	25.078224579557904	26.759592424249014	24.095791150271527
44-45	23.890446597883823	25.16510640801131	26.8820757130119	24.062371281092965
46-47	24.12247608625668	25.1554509189609	26.57247389467138	24.149599100111043
48-49	23.960913434256152	25.404597015342112	26.313055735501056	24.32143381490068
50-51	24.0234829807892	25.56427181533099	26.252809930547727	24.15943527333208
52-53	24.253493773183493	25.408388808940547	26.11439830021275	24.223719117663215
54-55	23.823609918469057	25.516434353217754	26.22691207730884	24.43304365100435
56-57	23.802495711500686	25.612676679248857	26.084694814814984	24.500132794435473
58-59	23.745377837305597	25.317636908416098	26.288818871869047	24.648166382409265
60-61	23.653274546006937	25.366452889369246	26.465042004978915	24.515230559644895
62-63	23.567079778309797	25.43004141643901	26.464754374678595	24.538124430572598
64-65	23.443282048042438	25.32613075580486	26.430527502742912	24.800059693409796
66-67	23.394739401845843	25.482602949001837	26.330169192829473	24.792488456322843
68-69	23.62007970315866	25.246115686874727	26.362635067673303	24.77116954229331
70-71	23.353352210083536	25.229472449687496	26.365957029949122	25.05121831027985
72-73	23.41664715322322	25.264003728269408	26.324447792440136	24.994901326067236
74-75	23.29739674687192	25.454415835747056	26.19569804570638	25.052489371674643
76-77	23.32590284342674	25.2162496827682	26.312790443021505	25.14505703078356
78-79	23.29875286255513	25.378261207376063	26.216742553294477	25.106243376774334
80-81	23.30492895510665	25.298689962628067	26.113185041019417	25.28319604124587
82-83	23.146847777441735	25.372558591347417	26.15640463437244	25.32418899683841
84-85	23.014606850859074	25.351039300610477	26.121100119578216	25.513253728952236
86-87	23.000718233226358	25.412217470997483	26.062151603392458	25.524912692383694
88-89	22.961617193622335	25.29451292304763	25.95214325211112	25.79172663121892
90-91	22.82261900797611	25.340401644588496	25.949483911890535	25.88749543554486
92-93	22.896670367694842	25.33162497870183	25.99878270006006	25.772921953543264
94-95	22.705370779841648	25.165092105100555	26.09315735103836	26.03637976401944
96-97	22.790846547532034	25.01124618742517	26.01637097003988	26.181536295002918
98-99	22.63160394281127	25.182506936310677	25.82565413600948	26.360234984868576
100-101	22.672082337856033	25.12881884472294	25.842609831340702	26.356488986080333
102-103	22.45739315660782	25.070134545035504	25.901072270365795	26.571400027990876
104-105	22.31152792275126	25.29593852763188	25.645709546447677	26.746824003169188
106-107	22.391959332059074	25.351125805427984	25.562118842474668	26.694796020038275
108-109	22.34786427454492	25.39245532850634	25.547819512084608	26.71186088486413
110-111	22.33489932302188	25.52303414736416	25.428163827881207	26.71390270173275
112-113	22.235795155786317	25.67847725493566	25.35058315234923	26.735144436928792
114-115	22.164769735268735	25.887935763471308	25.1620685733091	26.785225927950858
116-117	22.077124077107598	25.885773028084856	25.230199607019816	26.806903287787726
118-119	22.177521872087276	25.936424660038966	25.036368616784276	26.849684851089478
120-121	22.088904266449184	26.093088244312817	25.03713955410567	26.780867935132324
122-123	22.01588085446036	26.0899065336725	24.931371678540273	26.962840933326866
124-125	21.88079859354395	26.229178837348886	24.91980221196929	26.97022035713787
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	91.5
2	208.0
3	327.0
4	254.0
5	233.5
6	411.5
7	799.5
8	1233.5
9	1436.5
10	1265.0
11	1072.0
12	1155.5
13	1330.5
14	1544.5
15	1703.0
16	1871.0
17	2166.5
18	2477.5
19	2824.5
20	3309.0
21	3856.5
22	4622.5
23	5637.0
24	6918.0
25	8729.0
26	11507.0
27	15220.0
28	18760.5
29	22780.0
30	27636.0
31	33638.0
32	42622.5
33	48203.0
34	51453.0
35	57287.0
36	64229.0
37	72947.5
38	81534.5
39	89702.0
40	98271.0
41	107485.5
42	116763.5
43	126992.5
44	138515.0
45	151793.5
46	164418.0
47	172218.5
48	178150.0
49	183868.5
50	189205.0
51	192150.0
52	191972.0
53	192247.0
54	190160.0
55	180452.5
56	167747.0
57	154810.5
58	141949.0
59	127470.0
60	108402.5
61	90081.5
62	74364.5
63	57080.5
64	42063.0
65	32464.5
66	24817.0
67	19160.5
68	14744.5
69	11259.5
70	8861.0
71	6715.5
72	5077.5
73	4204.0
74	3354.5
75	2539.0
76	1810.5
77	1183.0
78	764.5
79	507.0
80	319.5
81	199.5
82	143.0
83	97.0
84	61.0
85	50.0
86	35.0
87	20.5
88	15.5
89	10.0
90	9.0
91	9.5
92	6.5
93	4.5
94	5.5
95	12.0
96	16.0
97	14.5
98	10.5
99	3.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1711371547938883
2	0.012859885144292715
3	0.0
4	0.004324765644610896
5	0.1438156194517433
6	0.07759407566600818
7	0.008466472425957838
8	0.012837002786384723
9	0.012974296933832688
10-11	0.005583295329550575
12-13	0.010846237648389231
14-15	0.011612796638307036
16-17	0.015342620977310081
18-19	0.019564416011335004
20-21	0.023317122708246044
22-23	0.02571977028858543
24-25	0.025593917320091465
26-27	0.02295100498171814
28-29	0.02189841651795041
30-31	0.025056181909253603
32-33	0.0246671818248177
34-35	0.02400359344548587
36-37	0.024690064182725697
38-39	0.019472886579703027
40-41	0.026852447005031147
42-43	0.023523063929417994
44-45	0.03216115403968579
46-47	0.027355858879007017
48-49	0.018317327505349323
50-51	0.014450209018898309
52-53	0.015811709314423963
54-55	0.018191474536855354
56-57	0.014724797313794242
58-59	0.013878150071198456
60-61	0.01731050375739758
62-63	0.015056591503460155
64-65	0.02717280001574306
66-67	0.02068565154882672
68-69	0.019255504179577082
70-71	0.02599435858348136
72-73	0.031943771639559845
74-75	0.016543944767479776
76-77	0.007917295836165978
78-79	0.01401544421864642
80-81	0.016555385946433774
82-83	0.01598332699873392
84-85	0.01496506207182818
86-87	0.009805090363575497
88-89	0.02130347521234256
90-91	0.01872920994769322
92-93	0.015388385693126071
94-95	0.011372531880273096
96-97	0.02106321045430862
98-99	0.02200138712853638
100-101	0.028671594458716677
102-103	0.02055979858033275
104-105	0.01386670889224446
106-107	0.019232621821669092
108-109	0.010205531626965393
110-111	0.024186652308749824
112-113	0.01785968034718944
114-115	0.013226002870820623
116-117	0.01128100244864112
118-119	0.007780001688718014
120-121	0.004004412633898978
122-123	0.0060180601298024635
124-125	0.0010525884637677312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4370179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.69606164220979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.90384817378386	31.486344935346917
2	14.712665133634959	13.446217057355478
3	6.162764510941498	8.448422009352168
4	3.1040891285372423	5.673785926422044
5	1.8623057744571971	4.255001973311966
6	1.1799899340656168	3.2352535658549697
7	0.7764731377186892	2.483723505529929
8	0.5662177857180595	2.0699138271270385
9	0.43130361837710773	1.7737989058681598
>10	2.1260798331807313	17.32420295675451
>50	0.1199383648256687	3.750486042968646
>100	0.050311112601995174	3.929091351024202
>500	0.00255860153195898	0.769205857172074
>1k	0.0013545533410352626	1.0346553285275097
>5k	1.003372845211212E-4	0.3198967573843842
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7678	0.17569074401757914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6276	0.14360967823057133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8305886326395326E-4
2	0.0	0.0	0.0	0.0	1.8305886326395326E-4
3	0.0	0.0	0.0	0.0	1.8305886326395326E-4
4	0.0	0.0	0.0	0.0	1.8305886326395326E-4
5	0.0	0.0	0.0	0.0	2.0594122117194741E-4
6	0.0	0.0	0.0	0.0	2.7458829489592987E-4
7	0.0	0.0	0.0	0.0	2.7458829489592987E-4
8	0.0	0.0	0.0	0.0	2.7458829489592987E-4
9	0.0	0.0	0.0	0.0	2.7458829489592987E-4
10-11	0.0	0.0	0.0	0.0	2.7458829489592987E-4
12-13	0.0	0.0	0.0	1.1441178953997079E-5	7.20794274101816E-4
14-15	0.0	0.0	0.0	4.5764715815988316E-5	9.725002110897517E-4
16-17	0.0	0.0	0.0	5.720589476998539E-5	0.0012242061480776875
18-19	0.0	0.0	0.0	1.0297061058597371E-4	0.001304294400755667
20-21	0.0	0.0	0.0	1.1441178953997078E-4	0.0014987944429736173
22-23	0.0	0.0	0.0	1.1441178953997078E-4	0.0015560003377436027
24-25	0.0	0.0	0.0	3.661177265279065E-4	0.0015788826956515969
26-27	0.0	0.0	0.0	8.237648846877897E-4	0.00176194155891555
28-29	0.0	0.0	0.0	0.003043353601763223	0.0017848239168235442
30-31	0.0	0.0	0.0	0.013088708723372658	0.0018305886326395325
32-33	0.0	0.0	0.0	0.03688636094768658	0.0019221180642715093
34-35	0.0	0.0	0.0	0.07758263448705419	0.002059412211719474
36-37	0.0	0.0	0.0	0.14588647284241676	0.002059412211719474
38-39	0.0	0.0	0.0	0.2606987036457774	0.0021166181064894594
40-41	0.0	0.0	0.0	0.3961393801031948	0.0021280592854434565
42-43	0.0	0.0	0.0	0.5312711447288544	0.002173824001259445
44-45	0.0	0.0	0.0	0.6993077400262095	0.0022539122539374243
46-47	0.0	0.0	0.0	0.9017250780803258	0.0023454416855694014
48-49	0.0	0.0	0.0	1.1208694197651858	0.002425529938247381
50-51	0.0	0.0	0.0	1.3609053542200447	0.00258570644360334
52-53	0.0	2.2882357907994158E-5	0.0	1.602085406570303	0.002723000591051305
54-55	0.0	2.2882357907994158E-5	0.0	1.8734244066432977	0.002768765306867293
56-57	0.0	2.2882357907994158E-5	0.0	2.205641919930511	0.0028374123805912756
58-59	0.0	2.2882357907994158E-5	0.0	2.5674234396348523	0.002906059454315258
60-61	0.0	2.2882357907994158E-5	0.0	2.96010758369394	0.0029747065280392405
62-63	0.0	2.2882357907994158E-5	0.0	3.360743347126056	0.0033179418966591528
64-65	0.0	2.2882357907994158E-5	0.0	3.787259057352113	0.0033980301493371323
66-67	0.0	2.2882357907994158E-5	0.0	4.298874256638001	0.0034437948651531206
68-69	0.0	2.2882357907994158E-5	0.0	4.8681987625678484	0.003478118402015112
70-71	0.0	2.2882357907994158E-5	0.0	5.4903929564441185	0.0035467654757390943
72-73	0.0	2.2882357907994158E-5	0.0	6.116534357059516	0.00379847141272703
74-75	0.0	2.2882357907994158E-5	0.0	6.761576585306917	0.00407305970762296
76-77	0.0	2.2882357907994158E-5	0.0	7.487290566358952	0.004198912676116927
78-79	0.0	2.2882357907994158E-5	0.0	8.30421362603225	0.004256118570886913
80-81	0.0	2.2882357907994158E-5	0.0	9.16426993036212	0.004279000928794907
82-83	0.0	2.2882357907994158E-5	0.0	10.054176270582968	0.004530706865782844
84-85	0.0	2.2882357907994158E-5	0.0	10.946096258299718	0.004599353939506826
86-87	0.0	2.2882357907994158E-5	0.0	11.878003166460687	0.0047252069080007935
88-89	0.0	2.2882357907994158E-5	0.0	12.88244714919	0.004851059876494762
90-91	0.0	2.2882357907994158E-5	0.0	13.952128734315002	0.004851059876494762
92-93	0.0	2.2882357907994158E-5	0.0	15.033308704288771	0.005079883455574703
94-95	0.0	2.2882357907994158E-5	0.0	16.109157084869977	0.005102765813482697
96-97	0.0	2.2882357907994158E-5	0.0	17.208242499906753	0.005411677645240618
98-99	0.0	2.2882357907994158E-5	0.0	18.36328672120753	0.005674824761182551
100-101	0.0	2.2882357907994158E-5	0.0	19.598739548196995	0.005686265940136548
102-103	0.0	2.2882357907994158E-5	0.0	20.830222286089427	0.005754913013860531
104-105	0.0	2.2882357907994158E-5	0.0	22.040241372264155	0.005812118908630516
106-107	0.0	2.2882357907994158E-5	0.0	23.249036252290807	0.005812118908630516
108-109	0.0	2.2882357907994158E-5	0.0	24.4909991101051	0.005857883624446504
110-111	0.0	2.2882357907994158E-5	0.0	25.773589594385037	0.005926530698170487
112-113	0.0	2.2882357907994158E-5	0.0	27.073593553032953	0.005972295413986475
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6925	0.0	53.235004	1
GTATCAA	11460	0.0	47.159824	1
TCAACGC	14395	0.0	36.92822	4
TATCAAC	14645	0.0	36.421158	2
ATCAACG	14875	0.0	35.736584	3
CAACGCA	14960	0.0	35.65304	5
AACGCAG	15540	0.0	34.320576	6
GTACATG	15765	0.0	31.481686	1
ACGCAGA	17635	0.0	29.987518	7
ACATGGG	15860	0.0	29.834757	3
TACATGG	16295	0.0	29.770775	2
CGCAGAG	18200	0.0	29.056587	8
CATGGGG	11295	0.0	28.54409	4
GCAGAGT	19890	0.0	25.779339	9
GAGTACT	11555	0.0	23.553354	12-13
AGAGTAC	18550	0.0	23.072447	10-11
CAGAGTA	19735	0.0	21.867899	10-11
GTACTTT	12520	0.0	21.69179	14-15
AGTACTT	12860	0.0	21.278866	12-13
ATGGGGA	6185	0.0	19.656021	5
>>END_MODULE