FastQCFastQC Report
Mon 15 Aug 2016
SRR2921077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6549493
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63800.044.2208861
GTATCAA98000.043.7612531
TCAACGC109350.037.8858154
CAACGCA113000.036.556165
ATCAACG117200.035.2974553
GTACATG216850.033.997421
AACGCAG121850.033.7498866
TATCAAC125500.033.1996772
TACATGG222150.031.9917142
ACATGGG222550.031.1858183
CATGGGG169300.029.6385364
ACGCAGA142650.028.5349777
CGCAGAG149750.027.22168
GCAGAGT180250.021.6908559
GTATACG4550.020.974691
AGAGTAC149750.020.54315410-11
GTATATA28200.019.6707441
CAGAGTA165300.019.45656810-11
ATGGGGA97550.019.0373655
GAGTACA154400.018.9679831
Produced by FastQC (version 0.11.5)