FastQCFastQC Report
Mon 15 Aug 2016
SRR2921077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6549493
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.048.2647481
GTATCAA132400.047.440141
TCAACGC157750.039.2511984
CAACGCA162450.038.042215
ATCAACG164300.037.795223
AACGCAG171500.036.338586
TATCAAC173100.036.1147962
GTACATG224950.031.9526311
ACGCAGA200650.030.8938397
TACATGG231050.030.4356612
ACATGGG226850.029.8695323
CGCAGAG210900.029.3928058
CATGGGG173300.027.2353744
GCAGAGT238800.025.260219
AGAGTAC210150.023.5794710-11
CAGAGTA225250.021.40457310-11
GAGTACT124450.020.79310612-13
AGTACTT138400.019.6643712-13
ATGGGGG87050.019.6480675
GTACCGT10650.019.5615776
Produced by FastQC (version 0.11.5)