FastQCFastQC Report
Mon 15 Aug 2016
SRR2921076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5538561
Sequences flagged as poor quality0
Sequence length125
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74950.038.0498771
GTATCAA107050.037.8425251
GTACATG182500.035.2086871
TACATGG193350.032.942282
ACATGGG200700.030.6980613
TCAACGC128050.030.5424424
AACGCAG132250.030.0158026
CAACGCA132950.029.4612795
ATCAACG136050.029.3590473
CATGGGG144550.028.7857154
TATCAAC142050.028.1606122
ACGCAGA147300.026.5024227
CGCAGAG157450.024.5289338
GTATAGG11800.021.2355861
GCAGAGT174950.021.0549079
AGAGTAC150900.020.62159710-11
ATGGGGG76600.019.6610095
ATGGGGA80300.019.5705225
ATACCGT9150.019.5128176
TATACCG4657.2759576E-1219.2022615
Produced by FastQC (version 0.11.5)