FastQCFastQC Report
Mon 15 Aug 2016
SRR2921076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5538561
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100330.18114813577028402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85970.15522082360382056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.10020653379099734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87500.055.1616971
GTATCAA145200.048.2801321
TCAACGC180150.038.3437234
ATCAACG186750.037.0205153
CAACGCA186950.036.8852735
TATCAAC190650.036.451112
AACGCAG192900.035.8588836
GTACATG177150.032.9041441
ACGCAGA220000.031.231437
TACATGG187000.030.6288592
ACATGGG190550.029.7451823
CGCAGAG230100.029.7057048
CATGGGG143100.027.6133424
GCAGAGT250350.026.5648379
GAGTACT151750.024.14775312-13
CAGAGTA242100.023.30513410-11
AGAGTAC228150.023.27002110-11
GTACTTT170000.021.5916814-15
AGTACTT165600.021.5174712-13
ATGGGGG77350.020.8811345
Produced by FastQC (version 0.11.5)