FastQCFastQC Report
Mon 15 Aug 2016
SRR2921075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531149
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT61350.13539612138113313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102200.036.4853631
GGTATCA70900.032.7335321
TACATGG170200.031.262582
CATGGGG114000.029.3940894
TCAACGC123900.029.2551564
ACATGGG180900.029.1179733
CAACGCA124650.029.0307435
ATCAACG125400.029.0001413
TATCAAC127600.028.732732
AACGCAG125200.028.7102036
GTACATG193350.028.5730321
ACGCAGA139300.025.5036817
CGCAGAG140800.025.0629238
GTATAAT41000.022.8457451
GCAGAGT158000.020.978439
ATGGGGA63400.020.5589875
ATGGGGC49150.020.4649185
AGAGTAC136950.019.65945410-11
GTGTAGC47100.019.253611
TAATGGA46200.019.0667294
Produced by FastQC (version 0.11.5)