##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921075_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4531149
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.134448900267902	31.0	30.0	34.0	22.0	34.0
2	29.153398619202324	31.0	30.0	34.0	20.0	34.0
3	29.316260401059424	31.0	30.0	34.0	22.0	34.0
4	32.643200874656735	37.0	35.0	37.0	22.0	37.0
5	32.75200749302219	37.0	35.0	37.0	25.0	37.0
6	32.687494055039906	37.0	35.0	37.0	24.0	37.0
7	32.5888312213966	37.0	35.0	37.0	23.0	37.0
8	32.578410464983605	37.0	35.0	37.0	22.0	37.0
9	33.93448902254152	39.0	35.0	39.0	19.0	39.0
10-11	33.88557096665768	39.0	35.0	39.0	20.0	39.0
12-13	33.787971880862884	39.0	35.0	39.0	18.5	39.0
14-15	34.899991370842145	40.0	35.0	41.0	18.5	41.0
16-17	34.85220878854348	40.0	35.0	41.0	18.0	41.0
18-19	34.88185182169026	40.0	35.0	41.0	19.0	41.0
20-21	34.92782503952088	40.0	35.0	41.0	19.0	41.0
22-23	34.83584461689519	40.0	34.0	41.0	19.0	41.0
24-25	34.81413224327869	40.0	34.0	41.0	19.0	41.0
26-27	34.75064382124711	40.0	34.0	41.0	19.0	41.0
28-29	34.61153903789083	40.0	34.0	41.0	18.0	41.0
30-31	34.50267503893604	40.0	34.0	41.0	17.5	41.0
32-33	34.378973523051215	39.5	34.0	41.0	16.5	41.0
34-35	34.257782407949946	39.0	34.0	41.0	16.0	41.0
36-37	34.12834338486772	39.0	34.0	41.0	15.0	41.0
38-39	33.9759982512162	39.0	33.0	41.0	10.5	41.0
40-41	33.856552830198254	39.0	33.0	41.0	9.0	41.0
42-43	33.648396687021325	39.0	33.0	41.0	8.5	41.0
44-45	33.45268562124088	39.0	33.0	41.0	7.5	41.0
46-47	33.27916329831572	38.0	33.0	41.0	2.0	41.0
48-49	33.045222414888585	38.0	32.0	40.0	2.0	41.0
50-51	32.88472890650914	38.0	32.0	40.0	2.0	41.0
52-53	32.71122346671893	38.0	32.0	40.0	2.0	41.0
54-55	32.49308740454132	38.0	31.5	40.0	2.0	41.0
56-57	32.2611403862464	38.0	31.0	40.0	2.0	41.0
58-59	32.00238140480483	37.0	31.0	40.0	2.0	41.0
60-61	31.72406667712759	37.0	31.0	40.0	2.0	41.0
62-63	31.46362953414244	36.0	30.5	40.0	2.0	41.0
64-65	31.172802086181672	36.0	30.0	39.5	2.0	41.0
66-67	30.847068922253495	35.5	30.0	39.0	2.0	41.0
68-69	30.490404751642465	35.0	29.0	39.0	2.0	40.0
70-71	30.14583850586242	35.0	29.0	38.0	2.0	40.0
72-73	29.793395118986375	35.0	29.0	37.5	2.0	40.0
74-75	29.754408870686	35.0	29.0	37.0	2.0	39.5
76-77	29.790937795247963	35.0	30.0	37.0	2.0	39.0
78-79	29.593305252155687	35.0	30.0	36.0	2.0	39.0
80-81	29.351535780438912	35.0	30.0	36.0	2.0	38.5
82-83	29.07430477346916	35.0	29.0	35.5	2.0	37.0
84-85	28.78658293955904	35.0	29.0	35.0	2.0	37.0
86-87	28.60763881302513	35.0	29.0	35.0	2.0	36.5
88-89	28.402062810117258	34.0	29.0	35.0	2.0	36.0
90-91	28.235157682962974	34.0	29.0	35.0	2.0	36.0
92-93	28.102416738006188	34.0	29.0	35.0	2.0	35.5
94-95	27.9691882787346	34.0	29.0	35.0	2.0	35.0
96-97	27.859589697888993	34.0	29.0	35.0	2.0	35.0
98-99	27.748179876671458	34.0	28.0	35.0	2.0	35.0
100-101	27.612096181343848	34.0	27.5	35.0	2.0	35.0
102-103	26.33957424485489	32.5	24.5	34.5	2.0	35.0
104-105	27.277184992151	34.0	27.0	35.0	2.0	35.0
106-107	27.418109843662172	34.0	27.0	35.0	2.0	35.0
108-109	27.36291368922099	34.0	27.0	35.0	2.0	35.0
110-111	27.26940661187703	34.0	27.0	35.0	2.0	35.0
112-113	27.159619888906764	34.0	27.0	35.0	2.0	35.0
114-115	27.027904180595254	34.0	25.5	35.0	2.0	35.0
116-117	26.893289428354706	34.0	25.0	35.0	2.0	35.0
118-119	26.69507656887911	34.0	25.0	35.0	2.0	35.0
120-121	26.493395163125292	34.0	25.0	35.0	2.0	35.0
122-123	26.19585573107395	34.0	24.5	35.0	2.0	35.0
124-125	24.966274337921796	32.5	12.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	246199.0
3	33752.0
4	21428.0
5	20231.0
6	25274.0
7	26125.0
8	25255.0
9	27056.0
10	28986.0
11	31147.0
12	31969.0
13	31004.0
14	30485.0
15	26983.0
16	22900.0
17	20598.0
18	19469.0
19	19247.0
20	19676.0
21	20797.0
22	24482.0
23	30952.0
24	40792.0
25	51645.0
26	57206.0
27	63232.0
28	73201.0
29	85016.0
30	99935.0
31	118411.0
32	147164.0
33	186470.0
34	251914.0
35	371745.0
36	670686.0
37	1104744.0
38	424207.0
39	766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87894331175987	19.07009628593678	12.234410147366315	24.816550254937038
2	15.583616196163577	21.218588311840616	39.05739923446485	24.140396257530956
3	18.598511763886034	25.517181818242058	30.139001138187645	25.745305279684267
4	12.061206160376363	16.694543072138924	37.92969314306693	33.31455762441779
5	12.670443349318402	39.13090349665674	34.98357752758949	13.215075626435366
6	31.4408627528456	37.72119466721825	17.42709567410356	13.410846905832589
7	26.654353356787396	33.68272847273565	21.938911016707596	17.72400715376936
8	25.31226419576047	35.99753410219556	20.386810098249235	18.30339160379474
9	25.82979148427083	16.095963816791127	19.7923328119814	38.28191188695664
10-11	24.371578313202438	27.049620906761774	28.50089882621272	20.077901953823066
12-13	24.625956687384814	24.66042802779532	28.137151534068533	22.576463750751333
14-15	22.269861564844707	26.224108649366645	25.72190909618109	25.78412068960756
16-17	22.226187864515985	28.61051847403852	26.410857332097027	22.752436329348473
18-19	22.139919550003125	27.867226799051952	27.890455194821406	22.102398456123513
20-21	23.758678810558383	27.289637560942204	27.064380593041932	21.887303035457485
22-23	22.883170863122054	27.048366476166834	27.049360369136267	23.019102291574846
24-25	22.53794551590947	27.056075623088866	27.56844749698647	22.837531364015195
26-27	22.39814091301425	27.208378262962547	27.409073145104973	22.984407678918235
28-29	22.542291109158313	27.619168169923462	27.282189024656944	22.556351696261277
30-31	22.9113196498299	27.335927071384347	27.169339816132204	22.58341346265355
32-33	22.42998510142694	27.5290570776175	27.20886146978046	22.832096351175096
34-35	22.649580296199392	27.494923107135747	27.12474565832605	22.730750938338808
36-37	22.744424806207718	27.170693388806384	27.354053550754458	22.730828254231444
38-39	22.359552199773113	27.542587582741085	27.315403006067974	22.782457211417828
40-41	22.721429729613902	27.482644419241375	27.186308343270476	22.60961750787424
42-43	22.61190602882113	27.343332222296585	27.332641530730328	22.712120218151956
44-45	22.61207244429592	27.482252154689252	27.18085739635835	22.724818004656484
46-47	22.478058200580858	27.392672326261952	26.98744430281776	23.141825170339434
48-49	22.289246100084533	27.25493324881883	27.464305413915852	22.99151523718079
50-51	22.170069582252495	27.562743844328345	27.283078625405416	22.984107948013744
52-53	22.54531417997114	27.407387772750692	26.954762303152595	23.092535744125573
54-55	22.538709620768483	27.21326627167042	27.05439299950286	23.193631108058234
56-57	22.403854533958643	27.525456402593296	26.88992311632341	23.180765947124645
58-59	22.35321243305326	27.221445748089117	27.224084913547454	23.201256905310167
60-61	22.397640615553442	27.217014463743226	27.198073032416026	23.187271888287302
62-63	22.08866205911165	27.087082038786214	27.269593439397745	23.554662462704396
64-65	22.12360890503987	27.02171657633647	27.32887679332332	23.52579772530034
66-67	22.187419348177002	27.371521128306647	26.96948369691557	23.471575826600777
68-69	22.528856359333048	26.957632425590848	27.064354844588166	23.449156370487934
70-71	22.164066906481413	26.46789896149854	27.488089057262094	23.87994507475795
72-73	22.302108067616462	26.50330335184871	27.479722304669835	23.714866275864992
74-75	21.999241180378714	26.87482845098627	27.29186997123223	23.83406039740278
76-77	21.710299876419334	26.650113396418234	27.661347440174715	23.978239286987712
78-79	22.190244535432374	26.804584245590107	27.386212475531273	23.618958743446246
80-81	22.071852959316576	26.565148085116913	27.395028690994366	23.967970264572145
82-83	22.046700421491643	26.43755226672019	27.66311331209546	23.85263399969271
84-85	22.376107279265185	26.32572228249121	27.381833047919386	23.91633739032422
86-87	21.827545779211636	26.07596264878002	27.74153680312302	24.354954768885325
88-89	22.12556675843163	26.167535343829307	27.61357612051183	24.093321777227235
90-91	22.05632926044789	26.219811224057004	27.673372289062943	24.050487226432164
92-93	22.173173399104424	26.077400581445172	27.515134623725825	24.234291395724586
94-95	21.814275710173614	26.246435048854465	27.602784362152644	24.336504878819277
96-97	21.84156694248611	25.995778440580867	27.829236684538493	24.33341793239453
98-99	21.754817089951555	26.09183249306575	27.575611934641568	24.577738482341125
100-101	21.739482448186664	26.246861852128273	27.631750297039524	24.381905402645536
102-103	21.702208245086716	26.0807901007723	27.789402897065614	24.427598757075373
104-105	21.400802702650175	26.152062944044047	27.762161572399606	24.684972780906172
106-107	21.30178429774686	26.221586660478447	27.754574398829746	24.72205464294495
108-109	21.233311208454687	26.249373561068257	27.754848391561087	24.762466838915973
110-111	21.18918659807228	26.42807960677735	27.598471890593583	24.784261904556782
112-113	21.307565524275933	26.624782491342724	27.490443755209938	24.577208229171408
114-115	21.140566833941733	26.68493279598248	27.51504906748744	24.65945130258834
116-117	21.058670099640477	26.58404674452119	27.75330036429696	24.603982791541384
118-119	21.154659359210484	26.502626600231633	27.52289626533196	24.819817775225925
120-121	21.120381801459924	26.705620137375615	27.4541653202531	24.719832740911365
122-123	21.261850035951873	26.588934775237277	27.390512460555104	24.758702728255745
124-125	21.30080970286811	26.71348882807129	27.28217989736671	24.703521571693884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2701.0
1	2009.5
2	1157.5
3	892.0
4	709.0
5	592.5
6	544.0
7	550.5
8	619.0
9	718.5
10	776.0
11	818.0
12	927.5
13	1066.0
14	1221.5
15	1455.0
16	1739.0
17	2073.5
18	2432.0
19	2913.0
20	3610.5
21	4597.5
22	5938.0
23	8120.5
24	10744.5
25	13631.0
26	19543.5
27	28007.5
28	34483.5
29	42075.5
30	49225.0
31	55555.5
32	64329.0
33	74858.5
34	88021.0
35	99713.0
36	114527.0
37	125854.0
38	129162.0
39	132913.5
40	140930.5
41	148368.0
42	150937.5
43	156965.5
44	165253.5
45	170184.0
46	172724.0
47	174543.0
48	175378.0
49	176547.5
50	178296.5
51	175601.5
52	166420.5
53	159093.0
54	151739.0
55	139629.0
56	126011.5
57	112238.0
58	103381.5
59	95621.5
60	81965.0
61	65741.0
62	51003.5
63	38300.5
64	28724.0
65	22424.5
66	16955.0
67	12809.0
68	9986.5
69	7536.0
70	5798.0
71	4508.5
72	3592.0
73	2987.0
74	2399.5
75	1833.5
76	1318.5
77	870.5
78	598.0
79	466.5
80	364.0
81	272.0
82	221.5
83	172.5
84	138.0
85	124.5
86	98.0
87	75.5
88	61.0
89	47.5
90	38.5
91	31.0
92	23.5
93	27.0
94	30.0
95	27.5
96	31.0
97	32.5
98	31.5
99	35.5
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29932805122939016
2	0.07362371001262594
3	0.08333427128527444
4	0.08878542727242031
5	0.09765734916243099
6	0.1325491613716521
7	0.11968266768539282
8	0.11948404256845227
9	0.12656833840599813
10-11	0.10350575538345794
12-13	0.09269172123891754
14-15	0.10287677584647956
16-17	0.10527131197848492
18-19	0.09626697334384722
20-21	0.09531798667402021
22-23	0.07720999684627453
24-25	0.08973441394224732
26-27	0.10209331010743632
28-29	0.09514143101451751
30-31	0.09030821983563109
32-33	0.10021740622522014
34-35	0.09414830542981482
36-37	0.09414830542981482
38-39	0.08326806291296093
40-41	0.09628904280128506
42-43	0.08487913330592307
44-45	0.08161285360512312
46-47	0.0707215763595503
48-49	0.07341405016696648
50-51	0.07534512769277726
52-53	0.08842128122469599
54-55	0.0942476179882851
56-57	0.10230296995309579
58-59	0.07067743744467463
60-61	0.08907233021911219
62-63	0.09508625737092291
64-65	0.08540880028443117
66-67	0.0791300396433664
68-69	0.08792471843234465
70-71	0.08617019656603656
72-73	0.06667183091970712
74-75	0.09669732776388507
76-77	0.10043810079959851
78-79	0.08135905484458798
80-81	0.09474418078063644
82-83	0.09903669025229583
84-85	0.10334023445267415
86-87	0.0909592688300473
88-89	0.06831600549882602
90-91	0.09492073644013913
92-93	0.06887877666349088
94-95	0.09796632156656071
96-97	0.08633571749682034
98-99	0.0799576442972853
100-101	0.09698423071057695
102-103	0.0688346377486152
104-105	0.07602928087335023
106-107	0.09423658325956617
108-109	0.10086845521963635
110-111	0.09375105519593376
112-113	0.0826059791898258
114-115	0.09652077210438235
116-117	0.08988890014431218
118-119	0.08206527748259879
120-121	0.11556671387323612
122-123	0.09410416651493915
124-125	0.11541222767117126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4531149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82350945122102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.44102302273217	32.278548842358454
2	13.165855989692163	12.066114527541474
3	5.9245015494225095	8.144443582312078
4	3.193165985649196	5.852882868908537
5	2.0102438694635896	4.605821447581197
6	1.2864994176821556	3.537115093508916
7	0.8567500395542675	2.748150547439428
8	0.6015499540151897	2.2052104002557296
9	0.4383455235151377	1.8077877215726703
>10	1.91897791016599	14.82536767759479
>50	0.09844472368784708	3.101171675030461
>100	0.05735040791339006	5.067350926398297
>500	0.004442568824484772	1.433559792269303
>1k	0.002800748907584697	2.19072200349284
>5k	4.828877426869281E-5	0.13575289373587998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	6135	0.13539612138113313	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.310418615675627E-5	0.0
12-13	0.0	0.0	0.0	6.620837231351253E-5	0.0
14-15	0.0	0.0	0.0	8.827782975135005E-5	0.0
16-17	0.0	0.0	0.0	1.3241674462702508E-4	0.0
18-19	0.0	0.0	0.0	1.8759038822161884E-4	0.0
20-21	0.0	0.0	0.0	3.9725023388107524E-4	0.0
22-23	0.0	0.0	0.0	5.84840622102694E-4	0.0
24-25	0.0	0.0	0.0	0.0011807159729243068	0.0
26-27	0.0	0.0	0.0	0.0020083206268432137	0.0
28-29	0.0	0.0	0.0	0.006146343896437746	0.0
30-31	0.0	2.2069457437837512E-5	0.0	0.020888741464913204	0.0
32-33	0.0	2.2069457437837512E-5	0.0	0.056895061274745105	0.0
34-35	0.0	2.2069457437837512E-5	0.0	0.1143749631715929	0.0
36-37	0.0	2.2069457437837512E-5	0.0	0.21533169622098058	0.0
38-39	0.0	2.2069457437837512E-5	0.0	0.3813491897971133	0.0
40-41	0.0	2.2069457437837512E-5	0.0	0.5728900109001049	0.0
42-43	0.0	2.2069457437837512E-5	0.0	0.7570265290327023	0.0
44-45	2.2069457437837512E-5	2.2069457437837512E-5	0.0	0.9773128184484774	0.0
46-47	2.2069457437837512E-5	2.2069457437837512E-5	0.0	1.2358896165189006	0.0
48-49	2.2069457437837512E-5	2.2069457437837512E-5	0.0	1.5098046875086208	0.0
50-51	2.2069457437837512E-5	2.2069457437837512E-5	0.0	1.8056678339202705	0.0
52-53	2.2069457437837512E-5	2.2069457437837512E-5	0.0	2.0993350693168553	0.0
54-55	2.2069457437837512E-5	2.2069457437837512E-5	0.0	2.4245285246633914	0.0
56-57	2.2069457437837512E-5	2.2069457437837512E-5	0.0	2.8000293082394774	0.0
58-59	2.2069457437837512E-5	2.2069457437837512E-5	0.0	3.209053597663639	0.0
60-61	2.2069457437837512E-5	2.2069457437837512E-5	0.0	3.6440205343059784	0.0
62-63	2.2069457437837512E-5	2.2069457437837512E-5	0.0	4.0795171379268265	0.0
64-65	2.2069457437837512E-5	2.2069457437837512E-5	0.0	4.535218329832014	0.0
66-67	2.2069457437837512E-5	2.2069457437837512E-5	0.0	5.05522992071106	0.0
68-69	2.2069457437837512E-5	2.2069457437837512E-5	0.0	5.637620833038154	0.0
70-71	2.2069457437837512E-5	2.2069457437837512E-5	0.0	6.293039580027052	0.0
72-73	2.2069457437837512E-5	2.2069457437837512E-5	0.0	6.933351783399751	0.0
74-75	2.2069457437837512E-5	2.2069457437837512E-5	0.0	7.570110804124958	0.0
76-77	2.2069457437837512E-5	2.2069457437837512E-5	0.0	8.266490464118483	0.0
78-79	2.2069457437837512E-5	2.2069457437837512E-5	0.0	9.043611234148337	0.0
80-81	2.2069457437837512E-5	2.2069457437837512E-5	0.0	9.872142805279633	0.0
82-83	2.2069457437837512E-5	2.2069457437837512E-5	0.0	10.74870855052438	0.0
84-85	2.2069457437837512E-5	2.2069457437837512E-5	0.0	11.585405820907678	0.0
86-87	2.2069457437837512E-5	2.2069457437837512E-5	0.0	12.465888894847641	0.0
88-89	2.2069457437837512E-5	3.310418615675627E-5	0.0	13.412856209319093	0.0
90-91	2.2069457437837512E-5	4.4138914875675024E-5	0.0	14.421165580739014	0.0
92-93	2.2069457437837512E-5	4.4138914875675024E-5	0.0	15.453243757819484	0.0
94-95	2.2069457437837512E-5	4.4138914875675024E-5	0.0	16.478833514413232	0.0
96-97	2.2069457437837512E-5	4.4138914875675024E-5	0.0	17.500152830992754	0.0
98-99	2.2069457437837512E-5	4.4138914875675024E-5	0.0	18.58252730157406	0.0
100-101	2.2069457437837512E-5	4.4138914875675024E-5	0.0	19.736903377046307	0.0
102-103	2.2069457437837512E-5	4.4138914875675024E-5	0.0	20.9034948972104	0.0
104-105	2.2069457437837512E-5	4.4138914875675024E-5	0.0	22.04344858224702	0.0
106-107	2.2069457437837512E-5	6.620837231351253E-5	0.0	23.185079546048918	0.0
108-109	2.2069457437837512E-5	6.620837231351253E-5	0.0	24.3317202766892	0.0
110-111	2.2069457437837512E-5	6.620837231351253E-5	0.0	25.51958675382337	0.0
112-113	2.2069457437837512E-5	6.620837231351253E-5	0.0	26.74994797125409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10220	0.0	36.485363	1
GGTATCA	7090	0.0	32.733532	1
TACATGG	17020	0.0	31.26258	2
CATGGGG	11400	0.0	29.394089	4
TCAACGC	12390	0.0	29.255156	4
ACATGGG	18090	0.0	29.117973	3
CAACGCA	12465	0.0	29.030743	5
ATCAACG	12540	0.0	29.000141	3
TATCAAC	12760	0.0	28.73273	2
AACGCAG	12520	0.0	28.710203	6
GTACATG	19335	0.0	28.573032	1
ACGCAGA	13930	0.0	25.503681	7
CGCAGAG	14080	0.0	25.062923	8
GTATAAT	4100	0.0	22.845745	1
GCAGAGT	15800	0.0	20.97843	9
ATGGGGA	6340	0.0	20.558987	5
ATGGGGC	4915	0.0	20.464918	5
AGAGTAC	13695	0.0	19.659454	10-11
GTGTAGC	4710	0.0	19.25361	1
TAATGGA	4620	0.0	19.066729	4
>>END_MODULE