FastQCFastQC Report
Mon 15 Aug 2016
SRR2921075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531149
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87140.19231325211331607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74110.16355674907181378No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT64490.14232593101661412No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT62610.13817687301830064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.10908932811523081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92000.050.0048071
GTATCAA146000.042.4627271
ATCAACG181500.033.3989643
TCAACGC181750.033.3530244
TATCAAC184500.033.082042
CAACGCA184150.032.885975
AACGCAG188350.032.1426
CATGGGG114650.029.9458834
TACATGG180350.029.7781242
ACATGGG185450.028.219963
ACGCAGA213650.028.2056677
CGCAGAG216750.027.637598
GTACATG203400.027.223391
GCAGAGT239700.024.4950859
GAGTACT133050.023.65293312-13
ATGGGGA63650.023.5052035
GTATAAT40500.022.9834251
AGAGTAC218350.022.48997310-11
CAGAGTA229350.022.39693610-11
AGTACTT140100.022.12299212-13
Produced by FastQC (version 0.11.5)