##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4531149
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329660754921104	33.0	31.0	34.0	28.0	34.0
2	31.37435582012421	33.0	31.0	34.0	28.0	34.0
3	31.47982244680102	34.0	31.0	34.0	28.0	34.0
4	34.96994934397434	37.0	35.0	37.0	32.0	37.0
5	34.73884902041403	37.0	35.0	37.0	32.0	37.0
6	34.466697961157315	37.0	35.0	37.0	30.0	37.0
7	34.39727627584085	37.0	35.0	37.0	30.0	37.0
8	34.39335607811617	37.0	35.0	37.0	30.0	37.0
9	35.952969765505394	39.0	37.0	39.0	30.0	39.0
10-11	35.92920592547277	39.0	37.0	39.0	30.0	39.0
12-13	35.886720454348335	39.0	37.0	39.0	30.0	39.0
14-15	37.147556944165814	40.0	37.0	41.0	31.0	41.0
16-17	37.14411101908148	40.0	37.0	41.0	31.0	41.0
18-19	37.17345997670789	40.0	37.5	41.0	31.0	41.0
20-21	37.179111854410436	40.0	38.0	41.0	31.0	41.0
22-23	37.14717448046842	40.0	37.5	41.0	31.0	41.0
24-25	37.11189722518505	40.0	37.0	41.0	30.0	41.0
26-27	37.00569667870114	40.0	37.0	41.0	30.0	41.0
28-29	36.874111290535794	40.0	37.0	41.0	30.0	41.0
30-31	36.75184020653481	40.0	37.0	41.0	30.0	41.0
32-33	36.63518944091223	40.0	36.5	41.0	30.0	41.0
34-35	36.49155622558428	40.0	36.0	41.0	30.0	41.0
36-37	36.32988818068	40.0	36.0	41.0	29.0	41.0
38-39	36.188986060709986	40.0	36.0	41.0	28.0	41.0
40-41	36.07263290172095	40.0	35.5	41.0	28.0	41.0
42-43	35.932935443085185	40.0	35.0	41.0	27.0	41.0
44-45	35.772039939538516	40.0	35.0	41.0	27.0	41.0
46-47	35.525877652666026	40.0	35.0	41.0	25.5	41.0
48-49	35.29215139471246	40.0	35.0	41.0	24.5	41.0
50-51	35.11662439262095	39.5	35.0	41.0	24.0	41.0
52-53	34.93022001704203	39.0	35.0	41.0	24.0	41.0
54-55	34.741350372720035	39.0	35.0	41.0	23.0	41.0
56-57	34.51161250711464	39.0	34.0	41.0	22.5	41.0
58-59	34.28318126373686	38.0	34.0	41.0	22.0	41.0
60-61	34.0138714264307	38.0	34.0	40.5	21.5	41.0
62-63	33.736393682926774	37.5	34.0	40.0	20.0	41.0
64-65	33.44740042757367	37.0	33.0	40.0	19.5	41.0
66-67	33.118059459090844	36.5	33.0	40.0	18.0	41.0
68-69	32.7600352581652	36.0	33.0	39.0	15.5	41.0
70-71	32.37431554336439	35.5	32.5	39.0	12.5	41.0
72-73	31.994421503243437	35.0	32.0	38.5	8.0	40.0
74-75	31.669647588282793	35.0	32.0	37.0	8.0	40.0
76-77	31.359764487991896	35.0	32.0	37.0	7.0	39.0
78-79	31.042398407114838	35.0	31.5	36.5	7.0	39.0
80-81	30.976831042192607	35.0	32.0	36.0	3.5	38.5
82-83	30.741632972122524	35.0	32.0	36.0	2.0	37.0
84-85	30.511813008135462	35.0	32.0	35.0	2.0	37.0
86-87	30.310524328376754	35.0	31.0	35.0	2.0	36.5
88-89	30.110503318253272	35.0	31.0	35.0	2.0	36.0
90-91	29.945207275240783	35.0	31.0	35.0	2.0	36.0
92-93	29.81700833497199	35.0	31.0	35.0	2.0	36.0
94-95	29.69717327768299	35.0	31.0	35.0	2.0	35.0
96-97	29.62306414995402	35.0	31.0	35.0	2.0	35.0
98-99	29.522882275555276	35.0	31.0	35.0	2.0	35.0
100-101	29.419153177262544	35.0	31.0	35.0	2.0	35.0
102-103	28.11774265202932	33.0	28.0	34.5	2.0	35.0
104-105	29.11081284239384	34.0	30.0	35.0	2.0	35.0
106-107	29.264696658617936	35.0	31.0	35.0	2.0	35.0
108-109	29.255229854502687	35.0	31.0	35.0	2.0	35.0
110-111	29.158584279616495	35.0	31.0	35.0	2.0	35.0
112-113	29.03214030260316	35.0	30.0	35.0	2.0	35.0
114-115	28.918387477436738	35.0	30.0	35.0	2.0	35.0
116-117	28.795663528169122	34.5	30.0	35.0	2.0	35.0
118-119	28.633228569618876	34.0	30.0	35.0	2.0	35.0
120-121	28.72797738498557	35.0	30.5	35.0	2.0	35.0
122-123	28.601831124953073	35.0	31.0	35.0	2.0	35.0
124-125	27.909399690895178	34.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81624.0
3	31106.0
4	12689.0
5	11112.0
6	13329.0
7	15305.0
8	16594.0
9	18471.0
10	21708.0
11	24980.0
12	26959.0
13	31171.0
14	39210.0
15	36157.0
16	22507.0
17	19976.0
18	19359.0
19	20009.0
20	19907.0
21	20580.0
22	20433.0
23	20921.0
24	26524.0
25	34756.0
26	42930.0
27	50891.0
28	61299.0
29	73842.0
30	89175.0
31	108765.0
32	135480.0
33	175275.0
34	237304.0
35	352645.0
36	668793.0
37	1294952.0
38	632298.0
39	2113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01776225953205	19.53760108674888	12.780818634630215	24.663818019088858
2	15.936717264955455	21.312310896510564	38.74591995564373	24.005051882890253
3	18.492439776312807	25.53242014332347	30.8788565549268	25.096283525436924
4	12.448233882650813	16.763879410879294	37.8259245951627	32.961962111307194
5	12.599246277525559	38.66194921534578	35.361810878914845	13.376993628213823
6	31.509156664506545	37.81512270950865	17.144521881947785	13.531198744037026
7	26.69945909271394	33.3893876934819	21.88901060118246	18.0221426126217
8	25.80691371355454	35.40430278933708	20.428813312782268	18.35997018432611
9	26.25444872063138	14.924723806294912	19.962670918720956	38.85815655435275
10-11	24.72482361167023	26.25341211658933	28.677581908454634	20.3441823632858
12-13	25.248394133074655	23.816911581720294	28.57217586208609	22.362518423118967
14-15	22.663108362801317	25.384356632630876	26.304792961144635	25.647742043423165
16-17	22.21825279235044	28.006917623661625	26.99660023297868	22.77822935100925
18-19	22.05705601303229	26.94749888803905	28.983838765110804	22.011606333817852
20-21	23.54419915266631	26.367993589534873	28.348957554398723	21.738849703400096
22-23	22.734914246262814	26.278364517263054	28.338264373277795	22.64845686319634
24-25	22.423811559444296	26.37995815233174	28.83259530929515	22.363634978928815
26-27	22.242042766460372	26.544471944499143	28.72729737056113	22.48618791847936
28-29	22.371463937278673	26.720766589094623	28.657370898288654	22.25039857533805
30-31	22.820640685347364	26.43566568300742	28.590267954430264	22.153425677214955
32-33	22.442448580982578	26.668573936277767	28.583956065691314	22.30502141704834
34-35	22.479229180008183	26.725346150900002	28.55772794591121	22.2376967231806
36-37	22.69800234965746	26.32370218069581	28.589022304968935	22.389273164677785
38-39	22.296940612473495	26.61859299982043	28.557009687834245	22.527456699871827
40-41	22.56641749298992	26.535374023213727	28.5345987311878	22.363609752608554
42-43	22.606391726406297	26.32171652787206	28.600387410804455	22.471504334917192
44-45	22.51354504115282	26.602268758518772	28.30443371329946	22.579752487028948
46-47	22.623975345938884	26.487408889744934	27.916264936231787	22.97235082808439
48-49	22.38111924579404	26.446752469882146	28.274368685266133	22.897759599057686
50-51	22.225644705543992	26.895781998382738	28.043282654215645	22.835290641857622
52-53	22.535244687637267	26.830604771690354	27.756755921571052	22.87739461910133
54-55	22.451609926880852	26.768808250938037	27.694675825497196	23.08490599668392
56-57	22.33516319487402	27.121512393199982	27.454445585724365	23.088878826201633
58-59	22.161442184267255	26.94064697962905	27.837658365346595	23.060252470757103
60-61	22.26052176753775	27.055858572257897	27.72753660297062	22.956083057233737
62-63	21.913060138460754	27.002131243594047	27.742631063314082	23.342177554631117
64-65	21.877176846078612	26.909495853072002	27.811951183679973	23.401376117169413
66-67	22.003274231392776	27.202145766097413	27.41211333171531	23.3824666707945
68-69	22.316858892997075	26.83958942663208	27.437293747585674	23.406257932785167
70-71	22.04920556956716	26.341381574953505	27.731908012737378	23.87750484274196
72-73	22.18922987886205	26.379656091982852	27.608906652260913	23.82220737689419
74-75	21.941150119145334	26.803643791049847	27.27997127842042	23.975234811384404
76-77	21.730563415999875	26.62833693058558	27.521219888968172	24.119879764446374
78-79	22.230933587611027	26.823515389097846	27.133537726686797	23.812013296604334
80-81	22.055009553879675	26.64188717073936	27.059635567472203	24.243467707908767
82-83	21.99839551105092	26.535861122641208	27.231084484653667	24.234658881654205
84-85	22.25925046286527	26.425887699217476	26.945766846574177	24.369094991343072
86-87	21.7983264221687	26.23372151731102	27.221160027258406	24.746792033261876
88-89	21.956217163258742	26.391606773863362	27.03385541006455	24.618320652813345
90-91	21.877662308450375	26.419492233936914	27.060317017439743	24.642528440172967
92-93	21.960607598703113	26.32129033440046	26.801149525377667	24.916952541518764
94-95	21.63379458500703	26.51187701171776	26.84773441650556	25.006593986769648
96-97	21.678581015215517	26.18833706539706	27.13513484876312	24.997947070624303
98-99	21.671847982553867	26.281843128312453	26.763845680597097	25.282463208536576
100-101	21.665017027870785	26.392620214736723	26.742228843589405	25.200133913803093
102-103	21.708917943056743	26.135274722145514	26.797492096855763	25.35831523794198
104-105	21.487377015112397	26.28404836523591	26.67273739205213	25.55583722759956
106-107	21.409015365550164	26.3730577839958	26.618941022237653	25.598985828216374
108-109	21.340839817947074	26.496467788747356	26.563632388699176	25.599060004606393
110-111	21.282816049985563	26.607082465405785	26.325026247541917	25.78507523706674
112-113	21.301595423320148	26.90442801324689	26.133756678132503	25.66021988530046
114-115	21.174020782076468	26.975202795370613	26.113463873282594	25.737312549270325
116-117	21.109058896523138	26.97079000672537	26.248767549248917	25.671383547502575
118-119	21.233158877782714	26.97018437845727	25.968575929548283	25.828080814211734
120-121	21.110221799158392	27.26268125677766	25.870043016189587	25.757053927874363
122-123	21.178783486348888	27.15316030133369	25.645410388651595	26.02264582366583
124-125	21.055868277329402	27.351641562178507	25.63941766400642	25.953072496485674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	134.5
2	271.5
3	440.0
4	333.5
5	265.0
6	448.5
7	851.0
8	1375.5
9	1590.0
10	1403.5
11	1290.5
12	1395.5
13	1608.0
14	1848.5
15	2054.0
16	2335.0
17	2632.5
18	3058.5
19	3568.5
20	4035.0
21	4766.0
22	5919.0
23	7924.5
24	10363.5
25	13032.5
26	18593.0
27	26937.0
28	33253.5
29	41359.0
30	48688.5
31	54961.5
32	64213.5
33	74261.5
34	87020.5
35	98626.0
36	113793.0
37	125122.5
38	127913.5
39	131478.0
40	139521.5
41	146963.5
42	149576.5
43	156199.5
44	165306.0
45	170788.0
46	173177.0
47	175638.5
48	176865.0
49	178493.5
50	181442.0
51	178692.5
52	169951.0
53	163263.0
54	155514.0
55	142646.0
56	128516.5
57	115475.5
58	107288.0
59	98414.5
60	82208.5
61	64161.0
62	48907.5
63	36053.0
64	26825.0
65	20433.5
66	14885.0
67	11093.5
68	8428.5
69	6159.0
70	4696.5
71	3563.0
72	2694.0
73	2277.0
74	1809.5
75	1291.0
76	897.0
77	532.0
78	334.0
79	230.0
80	158.5
81	121.0
82	77.0
83	48.0
84	40.5
85	37.0
86	29.0
87	17.5
88	12.5
89	14.5
90	13.0
91	12.5
92	12.5
93	12.5
94	16.5
95	22.5
96	23.5
97	16.5
98	7.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18319856619148916
2	0.012380965622626843
3	0.0
4	0.00425940528550264
5	0.14724742002525187
6	0.08004592212703665
7	0.009445727783394454
8	0.014256869504843034
9	0.013969966558151145
10-11	0.00523046141276749
12-13	0.010957485617886325
14-15	0.011608534612302532
16-17	0.015261029818264639
18-19	0.01906801122629161
20-21	0.02331638178307533
22-23	0.02585436938842664
24-25	0.025324702409918545
26-27	0.02332741651179425
28-29	0.020999088752102393
30-31	0.0248281396175672
32-33	0.02456330612831315
34-35	0.02462951450062666
36-37	0.024872278532442876
38-39	0.01941008781657809
40-41	0.02743233559523203
42-43	0.02370259728823749
44-45	0.03193450491255088
46-47	0.027950967845021206
48-49	0.018130059285183516
50-51	0.014356182063313301
52-53	0.014985161600291669
54-55	0.01773280905130244
56-57	0.014576876637691676
58-59	0.013340987021172776
60-61	0.017192107344075423
62-63	0.01534930764801599
64-65	0.02713439791982122
66-67	0.020425282858718617
68-69	0.01984044223661592
70-71	0.02590954303202124
72-73	0.032629692821842755
74-75	0.016717614009161916
76-77	0.008298115996626904
78-79	0.014764467025913294
80-81	0.017445906104610553
82-83	0.01621001648809165
84-85	0.014852744855664644
86-87	0.009633318171616074
88-89	0.021451512629578062
90-91	0.019719060220707817
92-93	0.016717614009161916
94-95	0.01116714546354578
96-97	0.0228860273630375
98-99	0.02405570860724289
100-101	0.030224121961118473
102-103	0.021848762863459135
104-105	0.01464308501000519
106-107	0.020965984565945638
108-109	0.010780929958383624
110-111	0.026505418382842854
112-113	0.019376983630421334
114-115	0.014532737722816
116-117	0.011961645931307932
118-119	0.008529845299724198
120-121	0.003983537067529671
122-123	0.006433246843129635
124-125	0.0011807159729243068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	4531149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.59547498733569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.66605339447081	27.73006156474096
2	14.471547174365403	12.039017570387294
3	6.72724656373079	8.394690485259122
4	3.6822967753168325	6.126675336545526
5	2.2982919170251774	4.779927197410828
6	1.480546010265688	3.6950408722563646
7	0.9911786535491275	2.8859982824178663
8	0.687695096422379	2.2884003345720374
9	0.5179195146373358	1.9388797394895303
>10	2.2822083053575803	16.07562310758172
>50	0.1141760041491567	3.2806394022246463
>100	0.0711696518575378	5.763604128539577
>500	0.005632525317330967	1.6048034442963568
>1k	0.0038258654539874977	2.7593101802351407
>5k	2.1254808077704457E-4	0.6373283540429161
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8714	0.19231325211331607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7411	0.16355674907181378	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	6449	0.14232593101661412	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	6261	0.13817687301830064	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4943	0.10908932811523081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.310418615675627E-5	0.0
12-13	0.0	0.0	0.0	4.4138914875675024E-5	0.0
14-15	0.0	0.0	0.0	7.724310103243129E-5	0.0
16-17	0.0	0.0	0.0	1.3241674462702508E-4	0.0
18-19	0.0	0.0	0.0	1.8759038822161884E-4	0.0
20-21	0.0	0.0	0.0	3.5311131900540014E-4	0.0
22-23	0.0	0.0	0.0	5.40701707227019E-4	0.0
24-25	0.0	0.0	0.0	0.0011365770580486318	0.0
26-27	0.0	0.0	0.0	0.001975216440686457	0.0
28-29	0.0	2.2069457437837512E-5	0.0	0.00620151754003234	0.0
30-31	0.0	2.2069457437837512E-5	0.0	0.02103219293825915	0.0
32-33	0.0	2.2069457437837512E-5	0.0	0.05709368639168565	0.0
34-35	0.0	2.2069457437837512E-5	0.0	0.11494876906497667	0.0
36-37	0.0	2.2069457437837512E-5	0.0	0.21637999544927788	0.0
38-39	0.0	2.2069457437837512E-5	0.0	0.3831809547644538	0.0
40-41	0.0	2.2069457437837512E-5	0.0	0.5758252487393374	0.0
42-43	0.0	2.2069457437837512E-5	0.0	0.7608114409832913	0.0
44-45	0.0	2.2069457437837512E-5	0.0	0.9833046761428503	0.0
46-47	0.0	2.2069457437837512E-5	0.0	1.2440773852283384	0.0
48-49	0.0	2.2069457437837512E-5	0.0	1.5207511383977883	0.0
50-51	0.0	2.2069457437837512E-5	0.0	1.8200350507123029	0.0
52-53	0.0	2.2069457437837512E-5	0.0	2.1169133921660928	0.0
54-55	0.0	2.2069457437837512E-5	0.0	2.445185536825207	0.0
56-57	0.0	2.2069457437837512E-5	0.0	2.826887837941326	0.0
58-59	0.0	2.2069457437837512E-5	0.0	3.2417826030439523	0.0
60-61	0.0	2.2069457437837512E-5	0.0	3.683315203274048	0.0
62-63	0.0	2.2069457437837512E-5	0.0	4.1248257340467065	0.0
64-65	0.0	2.2069457437837512E-5	0.0	4.589387813113186	0.0
66-67	0.0	2.2069457437837512E-5	0.0	5.116847845877503	0.0
68-69	0.0	2.2069457437837512E-5	0.0	5.708673451259273	0.0
70-71	0.0	2.2069457437837512E-5	0.0	6.374674503089613	0.0
72-73	0.0	2.2069457437837512E-5	0.0	7.020713730667431	0.0
74-75	0.0	2.2069457437837512E-5	0.0	7.66011005155646	0.0
76-77	0.0	2.2069457437837512E-5	0.0	8.360892568308833	0.0
78-79	0.0	2.2069457437837512E-5	0.0	9.147591482866709	0.0
80-81	0.0	2.2069457437837512E-5	0.0	9.987312268918988	0.0
82-83	0.0	2.2069457437837512E-5	0.0	10.875596896063229	0.0
84-85	0.0	2.2069457437837512E-5	0.0	11.724344090207584	0.0
86-87	0.0	2.2069457437837512E-5	0.0	12.618278498455911	0.0
88-89	0.0	2.2069457437837512E-5	0.0	13.578344035916718	0.0
90-91	0.0	2.2069457437837512E-5	0.0	14.601340631261518	1.1034728718918756E-5
92-93	0.0	2.2069457437837512E-5	0.0	15.64801775443712	2.2069457437837512E-5
94-95	0.0	2.2069457437837512E-5	0.0	16.688890610306572	2.2069457437837512E-5
96-97	0.0	3.310418615675627E-5	0.0	17.724157823986808	2.2069457437837512E-5
98-99	0.0	4.4138914875675024E-5	0.0	18.821616768726873	2.2069457437837512E-5
100-101	0.0	4.4138914875675024E-5	0.0	19.995447070930574	2.2069457437837512E-5
102-103	0.0	4.4138914875675024E-5	0.0	21.180014164177784	2.2069457437837512E-5
104-105	0.0	4.4138914875675024E-5	0.0	22.33764548462211	2.2069457437837512E-5
106-107	0.0	4.4138914875675024E-5	0.0	23.496777528172217	2.2069457437837512E-5
108-109	0.0	4.4138914875675024E-5	0.0	24.662011776703878	2.2069457437837512E-5
110-111	0.0	4.4138914875675024E-5	0.0	25.869939390649037	2.2069457437837512E-5
112-113	0.0	4.4138914875675024E-5	0.0	27.12029553651844	2.2069457437837512E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9200	0.0	50.004807	1
GTATCAA	14600	0.0	42.462727	1
ATCAACG	18150	0.0	33.398964	3
TCAACGC	18175	0.0	33.353024	4
TATCAAC	18450	0.0	33.08204	2
CAACGCA	18415	0.0	32.88597	5
AACGCAG	18835	0.0	32.142	6
CATGGGG	11465	0.0	29.945883	4
TACATGG	18035	0.0	29.778124	2
ACATGGG	18545	0.0	28.21996	3
ACGCAGA	21365	0.0	28.205667	7
CGCAGAG	21675	0.0	27.63759	8
GTACATG	20340	0.0	27.22339	1
GCAGAGT	23970	0.0	24.495085	9
GAGTACT	13305	0.0	23.652933	12-13
ATGGGGA	6365	0.0	23.505203	5
GTATAAT	4050	0.0	22.983425	1
AGAGTAC	21835	0.0	22.489973	10-11
CAGAGTA	22935	0.0	22.396936	10-11
AGTACTT	14010	0.0	22.122992	12-13
>>END_MODULE