FastQCFastQC Report
Mon 15 Aug 2016
SRR2921074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664383
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138050.037.8623921
GGTATCA98850.035.9153981
GTACATG275250.033.2969321
TACATGG280100.031.8117282
TCAACGC157150.031.7403324
ACATGGG278150.030.9650153
CAACGCA163400.030.6353245
AACGCAG168900.029.8092296
CATGGGG199500.029.7762154
ATCAACG173000.028.9011443
TATCAAC175000.028.8767412
ACGCAGA190650.025.9693247
CGCAGAG203550.024.2648648
GCAGAGT228850.020.828089
ATGGGGA92700.020.3543915
ATGGGGC92850.019.8727665
AGAGTAC195600.019.63561210-11
CAGAGTA212600.018.68121110-11
GTATAGG18300.018.5850681
ATGGGGG100300.018.4560185
Produced by FastQC (version 0.11.5)