FastQCFastQC Report
Mon 15 Aug 2016
SRR2921074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664383
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87650.13152005219387902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76710.11510442902216153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.050.7481541
GTATCAA166950.044.7453041
TCAACGC205900.035.4908184
CAACGCA209800.034.8310785
TATCAAC216600.033.9857562
ATCAACG217900.033.5089453
AACGCAG219450.033.470166
GTACATG265050.031.2232571
TACATGG273300.029.6175122
ACATGGG272000.029.0573843
ACGCAGA253800.028.8286557
CATGGGG200500.028.5687314
CGCAGAG265750.027.4889898
GCAGAGT297600.024.3062559
AGAGTAC262200.022.61899810-11
CAGAGTA281650.021.97572510-11
GAGTACT153400.021.21268512-13
GTACTTT161650.020.18648514-15
ATGGGGA94800.020.0569215
AGTACTT163850.019.93240212-13
Produced by FastQC (version 0.11.5)